Detection of human papillomavirus DNA in squamous cell carcinoma of the esophagus by auto-nested PCR

Damin, Andréa Pires Souto; Frazzon, Ana Paula Guedes; Damin, Daniel de Carvalho; Biehl, H. Beck; Oliveira, Letícia; Auler, R.; Marroni, Cláudio Augusto; Alexandre, Cláudio Osmar Pereira

doi:10.1111/j.1442-2050.2006.00541.x

Cited by 26 publications

(19 citation statements)

References 21 publications

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“…The HPV frequency in esophageal tumors we detected is consistent with other studies conducted in Brazil in which the HPV16 and 18 prevalence found was 15.75% by using Nested PCR methodology 37 and 13.0% by using PCR followed by sequencing and in situ hybridization (HPV16 was the most frequent type) 38. However, this prevalence is higher than others that described HPV prevalence in Brazilian cases of esophageal cancers around 2.5% of low-risk HPV using hybrid capture methodology 39 and 0.0% using nested PCR methodology 40.…”

Section: Discussionsupporting

confidence: 91%

The Relation of HPV Infection and Expression of p53 and p16 Proteins in Esophageal Squamous Cells Carcinoma

et al. 2017

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GOAL: To investigate the HPV prevalence and characterize the expression of potential molecular surrogate markers of HPV infection in esophageal squamous cell carcinoma.MATERIALS AND METHODS: The prevalence of HPV in individuals with and without esophageal cancer (EC) was determined by using multiplex PCR; p16 and p53 protein levels were assessed by immunohistochemistry (IHC).RESULTS: High-risk HPV (hr-HPV) was found in the same frequency (13.8%) in esophageal squamous cell carcinoma (ESCC) and in healthy individuals. The p53 expression was positive in 67.5% of tumor tissue, 20.0% of adjacent non-tumoral tissue and 1.8% of normal esophageal tissue. p16 was positive in 11.6% of esophageal cancer cases and 4.7% of adjacent non-tumoral tissue. p16 was undetectable among control group samples. p53 and p16 levels were not significantly associated with the HPV status.CONCLUSIONS: These results suggest that hr-HPV types are not associated with the development of ESCC and that p53 and p16 protein expression have no relationship with HPV infection in normal or cancerous esophagus.

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Section: Discussionsupporting

confidence: 91%

The Relation of HPV Infection and Expression of p53 and p16 Proteins in Esophageal Squamous Cells Carcinoma

et al. 2017

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“…Thus, we may confirm that BE is an important risk factor for ADC, as previously demonstrated by various authors 10,15 . Tabagism alone was a more evident risk factor in ADC patients (22%).…”

Section: Discussionsupporting

confidence: 74%

“…Esophageal cancer is more frequent in older male individuals 5,6 , generally being associated with ethylism and tabagism 7 , intake of hot drinks and foods 8 , vitamin deficiencies 7 , ingestion of caustic substances 9 , Barrett Esophagus (BE) 10 , megaesophagus 11 and human papiloma virus infection 12 .…”

Section: Introductionmentioning

confidence: 99%

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Epidemiological features of esophageal cancer. Squamous cell carcinoma versus adenocarcinoma

et al. 2014

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PURPOSE:To analyze the epidemiological features of patients with esophageal cancer according to the histopathological types: squamous cell carcinoma or adenocarcinoma. METHODS:A total of 100 patients with esophageal cancer, being 50 squamous cell carcinomas and 50 adenocarcinomas were analyzed for demographics, nutritional factors, lifestyle habits, benign pathological conditions associated, like Barrett's esophagus and megaesophagus, tumor stage and survival rates. The nutritional factors evaluated included body mass index, percent weight loss, hemoglobin and albumin serum levels. RESULTS:Esophageal cancer occurred more often in men over 50 years-old in both histological groups. No significant differences on age and gender were found between the histological groups. Squamous cell carcinoma was significantly more frequent in blacks than adenocarcinoma. Alcohol consumption and smoking were significantly associated with squamous cell carcinoma. Higher values of body mass index were seen in patients with adenocarcinoma. Barrett's esophagus was found in nine patients (18%) with adenocarcinoma, and megaesophagus in two patients (4%) with squamous cell carcinoma. The majority of patients were on stages III and IV in both histological groups. The mean survival rates were 7.7 ± 9.5 months for patients with squamous cell carcinoma and 8.0 ± 10.9 months for patients with adenocarcinoma. No significant differences on tumor stage and survival rates were detected between the histological groups.CONCLUSION: Epidemiological features are distinct for the histopathological types of esophageal cancer. Squamous cell carcinoma is associated with black race, alcohol and smoking, while adenocarcinoma is related to higher body mass index, white race and Barrett's esophagus.

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“…DNA samples that did not yield PCR products with the E. faecalis-specific primers were reamplified in an autonested PCR using the same primer pair. The amplification parameters were modified by reducing the number of cycles to 30 cycles and using 2 l of template (67). The positive amplicons were validated by sequencing.…”

Section: Methodsmentioning

confidence: 99%

Enterococcus faecalis Enhances Cell Proliferation through Hydrogen Peroxide-Mediated Epidermal Growth Factor Receptor Activation

et al. 2012

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f Enterococcus faecalis is a member of the intestinal and oral microbiota that may affect the etiology of colorectal and oral cancers. The mechanisms by which E. faecalis may contribute to the initiation and progression of these cancers remain uncertain. Epidermal growth factor receptor (EGFR) signaling is postulated to play a crucial role in oral carcinogenesis. A link between E. faecalis and EGFR signaling in oral cancer has not been elucidated. The present study aimed to evaluate the association between E. faecalis and oral cancer and to determine the underlying mechanisms that link E. faecalis to EGFR signaling. We report the high frequency of E. faecalis infection in oral tumors and the clinical association with EGFR activation. Using human oral cancer cells, we support the clinical findings and demonstrate that E. faecalis can induce EGFR activation and cell proliferation. E. faecalis activates EGFR through production of H 2 O 2 , a signaling molecule that activates several signaling pathways. Inhibitors of H 2 O 2 (catalase) and EGFR (gefitinib) significantly blocked E. faecalis-induced EGFR activation and cell proliferation. Therefore, E. faecalis infection of oral tumor tissues suggests a possible association between E. faecalis infection and oral carcinogenesis. Interaction of E. faecalis with host cells and production of H 2 O 2 increase EGFR activation, thereby contributing to cell proliferation.

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Detection of human papillomavirus DNA in squamous cell carcinoma of the esophagus by auto-nested PCR

Cited by 26 publications

References 21 publications

The Relation of HPV Infection and Expression of p53 and p16 Proteins in Esophageal Squamous Cells Carcinoma

The Relation of HPV Infection and Expression of p53 and p16 Proteins in Esophageal Squamous Cells Carcinoma

Epidemiological features of esophageal cancer. Squamous cell carcinoma versus adenocarcinoma

Enterococcus faecalis Enhances Cell Proliferation through Hydrogen Peroxide-Mediated Epidermal Growth Factor Receptor Activation

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