Detection of human papillomavirus DNA in breast cancer of patients with cervical cancer history

Widschwendter, Andreas; Brunhuber, Thomas; Wiedemair, Annemarie; Marth, Christian

doi:10.1016/j.jcv.2004.06.009

Cited by 107 publications

(118 citation statements)

References 24 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This eliminates the possibility of ever knowing which cell type harbored the virus and allows for false-positive results caused by laboratory contamination with DNA from positive controls or circulating HPV virions, DNA, or infected cells from other body Cancer sites, such as the uterine cervix. 8,15 Of the 9 studies that were included in the meta-analysis, 8 used solution PCR techniques. 23 The molecular methods we used, ISH and IS-PCR, are free of these shortcomings.…”

Section: Discussionmentioning

confidence: 99%

Limited evidence of human papillomavirus on breast tissue using molecular in situ methods

Baltzell

Buehring

Krishnamurthy

et al. 2011

Cancer

View full text Add to dashboard Cite

BACKGROUND: Human papillomavirus (HPV) has been proposed as an etiologic agent of breast cancer based on numerous reports of high-risk (oncogenic) HPV types in malignant breast tissues. However, most of those studies used standard and nested solution polymerase chain reaction (PCR) techniques, both of which are disadvantaged by vulnerability to laboratory contamination from positive control DNA and the inability to localize the signal to a specific cell type. To overcome these drawbacks, the authors of this report explored the use of in situ molecular methods of viral detection to reassess the frequency of HPV in malignant breast tissue. METHODS: In situ hybridization (ISH) was used with probes that were specific for the capsid region of 12 oncogenic HPV types, and in situ PCR (IS-PCR) was used with primers that were specific for the capsid region of HPV-16, which is the most common oncogenic HPV type. These methods were resistant to molecular contamination and allowed identification of the positive cell type. The specimens examined were malignant tissues from patients with 70 breast cancer patients at The University of Texas M. D. Anderson Cancer Center in Houston, Texas. RESULTS: HPV was observed in 4 of 70 specimens (5.7%) using ISH and in 2 of 70 specimens (2.9%) of specimens using IS-PCR. Concordance between the 2 methods was high for negative specimens; both methods yielded negative results in 66 of 70 specimens (94.3%). However, there was no concordance for the few positive specimens, probably because of differences in sensitivity and the targeted HPV types. CONCLUSIONS: Oncogenic (high-risk) HPV types were present in malignant breast epithelium very infrequently and, thus, may be causative agents of only a relatively small proportion of all breast cancers. Cancer 2012;118:1212À20.

show abstract

Section: Discussionmentioning

confidence: 99%

Limited evidence of human papillomavirus on breast tissue using molecular in situ methods

Baltzell

Buehring

Krishnamurthy

et al. 2011

Cancer

View full text Add to dashboard Cite

show abstract

“…In the same study the presence of the low-risk HPV types 6 and 11 in breast tissues was also proved by in situ hybridization [16]. Additional studies have reported a high prevalence of HPV DNA in breast cancer of women with a history of cervical pre-malignant and malignant lesions [17][18][19], suggesting that HPV DNA may migrate through bloodstream from the original site of infection to the breast, or alternatively, may be transmitted by hand from the female perineum to the breast. In contrast to the studies cited above, no DNA from low-risk and high-risk HPV types was detected in several other independent studies using type-specific and/or consensus PCR primers in Paget's diseases, papillomas, papillary carcinomas and infiltrating ductal breast carcinomas [20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 75%

Analysis of the presence of cutaneous and mucosal papillomavirus types in ductal lavage fluid, milk and colostrum to evaluate its role in breast carcinogenesis

Cazzaniga

Gheit

Casadio

et al. 2008

Breast Cancer Res Treat

View full text Add to dashboard Cite

“…High-risk HPVs of the same type have been identified in both cervical and breast cancer that had occurred in the same women (Hennig et al, 1999;Widschwendter et al, 2004). This observation has lead to the hypothesis that HPVs may be transmitted to the breast during sexual activities (Kan et al, 2005).…”

Section: Sirmentioning

confidence: 95%

Are some breast cancers sexually transmitted?

Iacopetta

et al. 2006

Br J Cancer

View full text Add to dashboard Cite

Sir,High-risk human papilloma viruses (HPVs) have been consistently identified in 13 -86% of breast tumours in the 10 studies published since 1999 (Lawson et al, 2006). High-risk HPVs of the same type have been identified in both cervical and breast cancer that had occurred in the same women (Hennig et al, 1999;Widschwendter et al, 2004). This observation has lead to the hypothesis that HPVs may be transmitted to the breast during sexual activities (Kan et al, 2005). If this hypothesis is correct, it is likely that HPV-positive breast cancers would occur in women younger than those with HPV-negative breast cancer. This is because HPV genital infections are much more common in young women who have had multiple sexual partners (IARC, 1995).There are only two studies in which the age of women with HPV-positive and -negative breast cancer has been published. There were no differences in the average of age of women with either HPV-positive and -negative breast cancer in a study of Brazilian women (Damin et al, 2004). This is in contrast to a recent study of Greek women in which those with HPV-positive breast cancer were of average age 38 years as compared to average age 53 years for women with HPV-negative breast cancer (P-values for difference ¼ 0.001) (Kroupis et al, 2006).We have reviewed the ages of Australian women with HPVpositive and -negative breast cancer in our study published in this Journal (Kan et al, 2005). These data are shown in Table 1. The average age of women with HPV-positive breast cancer was 55.6 years as compared to 63.8 years for women with HPV-negative breast cancer (P-values for difference ¼ 0.049). These data are compatible with the hypothesis that HPV-positive breast cancers occur in younger women than those with HPV-negative breast cancers, and that high-risk HPVs may have been transmitted by sexual activity with HPV-positive sexual partners. HPV ¼ human papilloma virus. P-value for difference in average ages ¼ 0.049, which is significant at the 95% level. REFERENCES

show abstract

Detection of human papillomavirus DNA in breast cancer of patients with cervical cancer history

Cited by 107 publications

References 24 publications

Limited evidence of human papillomavirus on breast tissue using molecular in situ methods

Limited evidence of human papillomavirus on breast tissue using molecular in situ methods

Analysis of the presence of cutaneous and mucosal papillomavirus types in ductal lavage fluid, milk and colostrum to evaluate its role in breast carcinogenesis

Are some breast cancers sexually transmitted?

Contact Info

Product

Resources

About