Detection of human papillomavirus-16 DNA in archived clinical samples of breast and lung cancer patients from North Pakistan

Ilahi, Naureen Ehsan; Anwar, Sobia; Noreen, Mamoona; Hashmi, Shoaib Naiyar; Murad, Sheeba

doi:10.1007/s00432-016-2251-z

Cited by 19 publications

(11 citation statements)

References 40 publications

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“…The oncogenic potential of hr-HPVs has been associated with the occurrence of cervical, other ano-genital as well as head and neck cancers [ 23 , 24 ]. More recently, hr-HPV types have also been found in other tumors, such as breast [ 8 , 25 ], lung [ 8 , 9 ], colon [ 24 ], and oesophageal cancers [ 26 ]. In addition, some reports demonstrated the presence of HPV DNA in subjects with no recognized HPV-related disease or cancer, with viral nucleic acids being detected in sperm cells [ 27 , 28 ], placental tissue [ 29 , 30 ], or PBMCs [ 10 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus DNA detection in plasma and cervical samples of women with a recent history of low grade or precancerous cervical dysplasia

et al. 2017

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Circulating HPV DNA has been previously described in women with advanced stages of cervical cancer and has been suggested to be a prognostic marker of disease recurrences and metastases. Only a few studies have reported the presence of HPV DNA in bloodstream of patients with low grade or precancerous cervical lesions. This study aimed to define if HPV DNA could be detected in plasma samples of 120 women referred for a recent history of cervical dysplasia who presented with lesions ranging from High Squamous Intraepithelial Lesion (H-SIL) to regressed normal cytology. HPV DNA detection was carried out in both plasma and cervical samples using type-specific real-time quantitative PCR assays identifying oncogenic HPV 16, 18, 31, 33, 45, 51 and 52.Overall, 34.2% (41/120) of plasma samples were shown to be positive for HPV DNA detection; HPV 45 (46.3%), HPV-51 (29.6%), and HPV 16 (18.5%) were the most frequently identified genotypes. The rate of HPV detection in paired cervical and plasma samples increased with advancing disease stage, ranging from 15.4% in women with regressed lesions to 38.9% in women with HSIL; HPV 16 resulted the most common genotype identified in women found to be HPV DNA positive in both cervical and plasma samples. Moreover, HPV 16 showed the highest median viral load value in both cervical and plasma samples, with 48,313 copies/104 cells and 1,099 copies/ml, respectively.Results obtained in this study confirm that HPV DNA can be detected and quantified in plasma samples of women with asymptomatic cervical infection. Further knowledge on HPV dissemination through the blood stream of women with cervical lesions would be very important in better understanding the natural history of HPV infection as well as its potential role in other distant tumors.

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Section: Discussionmentioning

confidence: 99%

Human papillomavirus DNA detection in plasma and cervical samples of women with a recent history of low grade or precancerous cervical dysplasia

et al. 2017

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“…The associations of HPV with cervical carcinoma [119], middle ear carcinoma [120], penile carcinomas [121,122], oropharyngeal cancer [123], tongue cancer [124], esophageal squamous cell carcinoma (ESCC) [125], colorectal cancer (CRC) [126,127], lung cancer [128][129][130], and breast cancer have since been demonstrated [131].…”

Section: Pathogenic Stimulus By Virusmentioning

confidence: 99%

Chronic inflammation evoked by pathogenic stimulus during carcinogenesis

Brücher

Jamall

2019

4open

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A pathogenic (biological or chemical) stimulus is the earliest information received by a cell that can result in the disruption of homeostasis with consequent development of disease. Chronic inflammation involves many cell types with numerous cytokines and signaling pathways, the release of different components by the cells, and the crosstalk provoked by such stimuli involving subclinical chronic inflammation and is mechanistically manifold. Exosomes secrete chemicals that trigger the epithelium to produce exosome-like nanoparticles promoting chronic inflammation. Small molecules, together with various cytokines, selectively target signaling pathways inducing crosstalk that suppress apoptosis. 16S rRNA gene sequencing has become routine to provide information on the composition and abundance of bacteria found in human tissues and in reservoirs. The deregulation of autophagy with chronic stimulation of inflammation is an early phenomenon in carcinogenesis. The disruption of cell–cell integrity enables transcellular CagA migration and triggers deregulation of autophagy with the net result being chronic inflammation. The complex and insidious nature of chronic inflammation can be seen both inside and outside the cell and even with intracellular nuclear fragments such as chromatin, which itself can elicit a chronic inflammatory response within the cytoplasm and affect autophagy. The ultimate result of unresolved chronic inflammation is fibrosis, a step before tissue remodeling results in the formation of a precancerous niche (PCN). Various pathogenic stimuli associated with different neoplasms result in persistent inflammation. This ongoing disruption of homeostasis in the micromilieu of cells, tissues, and organs is an essential preamble to carcinogenesis and occurs early in that process.

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“…HPV is a small, double-stranded and circular DNA virus that infects epithelial cells. Accumulating evidence has indicated that various HPVs, including HPV16, may be involved in the pathogenesis and development of breast cancer ( 12 , 30 ). However, the prevalence of HPV16 may vary widely.…”

Section: Discussionmentioning

confidence: 99%

“…Polymerase chain reaction (PCR) has been employed for the diagnosis of HR-HPVs ( 11 ). However, the detection rate of HPV16 in breast carcinoma may vary by region ( 12 , 13 ). As ~80% of all breast cancers are invasive ductal carcinoma, the expression of HPV16 E7 was examined in samples from patients with invasive breast ductal carcinoma from North China in the present study ( 14 ).…”

Section: Introductionmentioning

confidence: 99%

HPV16 E7 increases COX‑2 expression and promotes the proliferation of breast cancer

et al. 2018

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Breast cancer remains the leading cause of mortality worldwide. Human papilloma virus 16 (HPV16) may serve a function in the pathogenesis and development of breast cancer. However, the detection rate of HPV16 in breast carcinoma may vary by region. In the present study, the expression of HPV16 E7 in paraffin-embedded tissues from patients with breast cancer from North China was detected. Additionally, the molecular mechanisms underlying the function of HPV16 E7 in the proliferation of breast cancer cells were examined. The results demonstrated that the DNA of HPV16 E7 was detected in 30.5% of the samples, and that HPV16 E7 promoted the proliferation of breast cancer cells in vitro and in vivo. Additionally, HPV16 E7-mediated proliferation of breast cancer cells was suppressed in response to treatment with cyclooxygenase-2 (COX-2)-specific small interfering RNA and celecoxib. The results of the present study revealed that HPV16 E7 may promote the proliferation of breast cancer cells by upregulating COX-2, suggesting that COX-2 may be a potential therapeutic target for HPV16 E7-mediated progression of breast cancer.

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Detection of human papillomavirus-16 DNA in archived clinical samples of breast and lung cancer patients from North Pakistan

Cited by 19 publications

References 40 publications

Human papillomavirus DNA detection in plasma and cervical samples of women with a recent history of low grade or precancerous cervical dysplasia

Human papillomavirus DNA detection in plasma and cervical samples of women with a recent history of low grade or precancerous cervical dysplasia

Chronic inflammation evoked by pathogenic stimulus during carcinogenesis

HPV16 E7 increases COX‑2 expression and promotes the proliferation of breast cancer

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