Detection of human papilloma virus and p16 expression in high-grade adenoid cystic carcinoma of the head and neck

Boland, Jennifer M.; McPhail, Ellen D.; García, Joaquín J.; Lewis, Jean E.; Schembri-Wismayer, David

doi:10.1038/modpathol.2011.186

Cited by 70 publications

(86 citation statements)

References 22 publications

(28 reference statements)

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“…therapeutic vaccines and de-intensification therapy). Recent studies have suggested that, like the oropharynx, the salivary glands may also be targeted by oncogenic HPV [13,[16][17][18]. These HPV-related forms of salivary gland cancer warrant considerable attention as they could provide new insights into a type of head and neck cancer in which little is known about the biologic mechanisms underlying tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

“…In light of potential clinical repercussions, claims regarding the identification of a new form of HPV-related head and neck cancer warrant careful corroboration. For example, the existence of a newly identified HPV-related adenoid cystic carcinoma either has been unconfirmed in subsequent studies or has been redefined as non-salivary gland carcinoma [5,16,19,20]. Recently, Isayeva et al [13].…”

Section: Discussionmentioning

confidence: 99%

“…In this study, p16 immunohistochemical staining was not used because p16 staining has been found to be a very poor surrogate marker for the presence of high risk HPV when dealing with salivary gland tumors [15,16]. In situ hybridization for HPV E6/E7 mRNA was performed manually using the RNAscope kit (Advanced Cell Diagnostics Inc., Hayward, CA, USA).…”

Section: Maml2 Fluorescence In Situ Hybridizationmentioning

confidence: 99%

“…In situ hybridization for HPV E6/E7 mRNA was performed manually using the RNAscope kit (Advanced Cell Diagnostics Inc., Hayward, CA, USA). The HPV HR (18) probe containing a cocktail of 18 high-risk HPV genotypes (16,18,26,31,33,35,39,45, 51, 52, 53, 56, 58, 59, 66, 68, 73, and 82) was used for HPV detection. A probe to the housekeeping gene PPiB (Peptidyl-prolyl cis-trans isomerase B) was used as a positive control to ensure presence of an intact RNA target in the specimen, and a known HPV-related squamous cell carcinoma included on the TMA to serve as a positive control (Fig.…”

Section: Maml2 Fluorescence In Situ Hybridizationmentioning

confidence: 99%

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Mucoepidermoid Carcinoma Does Not Harbor Transcriptionally Active High Risk Human Papillomavirus Even in the Absence of the MAML2 Translocation

Bishop

Yonescu

Batista

et al. 2014

Head and Neck Pathol

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High risk human papillomavirus (HPV) is firmly established as an important cause of oropharyngeal carcinoma. Recent studies have also implicated HPV as a cause of mucoepidermoid carcinoma (MEC)-a tumor of salivary gland origin that frequently harbors MAML2 translocations. The purpose of this study was to determine the prevalence of transcriptionally active HPV in a large group of MECs and to determine whether HPV infection and the MAML2 translocation are mutually exclusive events. Break-apart fluorescence in situ hybridization for MAML2 was performed on a tissue microarray containing 92 MECs. HPV testing was performed using RNA in situ hybridization targeting high risk HPV mRNA E6/E7 transcripts. Of the 71 MECs that could be evaluated by FISH, 57 (80 %) harbored the MAML2 rearrangement. HPV was not detected in any of the 57 MECs that contained a MAML2 rearrangement, in any of the 14 MECs that did not contain the rearrangement, or in any of the 21 MECs where MAML2 status was unknown. High risk HPV does not appear to play any significant role in the development of MEC. It neither complements nor replaces MAML2 translocation in the tumorigenesis of MEC.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Maml2 Fluorescence In Situ Hybridizationmentioning

confidence: 99%

Section: Maml2 Fluorescence In Situ Hybridizationmentioning

confidence: 99%

See 2 more Smart Citations

Mucoepidermoid Carcinoma Does Not Harbor Transcriptionally Active High Risk Human Papillomavirus Even in the Absence of the MAML2 Translocation

Bishop

Yonescu

Batista

et al. 2014

Head and Neck Pathol

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“…In a recent study, Boland et al [28] investigated the expression of p16 expression and the presence of HPV in adenoid cystic carcinoma of different head and neck sites. Using ISH for high-risk HPV, the authors found that only 2 of 25 (8 %) cases were positive for HPV.…”

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus-16 Associated Adenocarcinoma NOS of Base of Tongue

Perez-Ordoñez

Irish

et al. 2012

Head and Neck Pathol

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The causative role of human papillomavirus (HPV) in a subset of squamous cell carcinomas of the oropharynx (OSCC) is now well established with HPV-16 being detected in approximately 92 % of HPV-positive OSCC in North America. Recently HPV has also been found in some non-squamous oropharyngeal carcinomas including adenosquamous carcinomas, neuroendocrine carcinomas, and undifferentiated ''lymphoepithelioma-like'' carcinomas. Herein, we report a HPV-16 positive adenocarcinoma NOS arising in the base of the tongue of a non-smoker, 64 yearold male. To our knowledge, this is the first time this association has been clearly documented. The adenocarcinoma was diffusely positive for p16 and HPV-16 was demonstrated by in situ hybridization and linear array analysis. Q-PCR demonstrated a viral load of 4 copies per cancer cell, whereas qRT-PCR for E6/E7 transcript levels were 30 copies per 1,000 human ribosomal protein large P0 (RPLP0) reference gene. The presence of HPV in non-squamous oropharyngeal carcinomas raises the possibility that HPV is also involved in the carcinogenesis of some uncommon carcinomas of the region.

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