Detection of human Metapneumovirus infection in children under 18 years old hospitalized in Lima-Peru

Valle-Mendoza, Juana del; Orellana-Peralta, Fiorella; Valle, Luís J. del; Verne, Eduardo; Ugarte, Claudia; Weilg, Claudia; Silva-Caso, Wilmer; Valverde-Ezeta, Jorge; Carrillo-Ng, Hugo; Peña-Tuesta, Isaac; Palomares-Reyes, Carlos; Cornejo-Tapia, Angela; Aguilar-Luis, Miguel Ángel

doi:10.7717/peerj.7266

Cited by 4 publications

(3 citation statements)

References 60 publications

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“…However, even after successful treatment, half of PCR tests are still positive, which results in an inability to assess disease progression and monitor it dynamically due to hysteresis [24]. In addition, molecular diagnostic methods such as RT-PCR still require standardization [25]. So, we aimed to evaluate the predictive value of Th1/Th2 cytokines in HMPV pneumonia.…”

Section: Discussionmentioning

confidence: 99%

Profiles and Predictive Value of Cytokines in Children with Human Metapneumovirus Pneumonia

Xiang

Lin

Wang

et al. 2022

Preprint

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Background: Human metapneumovirus (HMPV) is an important cause of respiratory tract infections in young children. To evaluate the predicitive value of Th1/Th2 cytokines which include IL-2, IL-4, IL-6, IL-10, INF-γ and TNF-α in pneumonia caused by HMPV. Methods: A retrospective study was performed among 59 pneumonia pediatric patients with HMPV infection and 33 healthy children as the control cohort, which was detected by the immunofluorescence assay, and the Th1/Th2 cytokines were measured by flow cytometry. Results: When compared with the healthy children, children who were infected with HMPV pneumonia had a significantly lower level of IL-2 (p<0.001) and higher levels of IL-4 ((p<0.001), IL-6 (p=0.001), IL-10 (p<0.001), and IFN-γ (p<0.001). Compared with patients diagnosed with influenza virus A (IVA) or influenza virus B (IVB) infections, HMPV-positive patients had significantly higher levels of IL-4 (p<0.001 and <0.001), IFN-γ (p<0.001 and <0.001), and TNF-α (p<0.001 and 0.016). Moreover, compared with IVA patients, HMPV-positive patients had a significantly lower level of IL-6 (p=0.033). Finally, when comparing cytokine levels among the patients with HMPV pneumonia, IL-6 and TNF-α levels were found to be significantly higher in the severe group than the mild group (p=0.027 and 0.049). The IL-6 and TNF-α were used to differentiate between mild and severe symptoms in children diagnosed with HMPV pneumonia with an AUC of 0.678 (95% CI, 0.526-0.829) and 0.658 (95% CI, 0.506-0.809), respectively.Conclusions: Our study indicates that IL-2, IL-4, IL-6, IL-10, and IFN-γ may be a reference for auxiliary diagnose HMPV pneumonia in children. IL-4, TNF-α, and IFN-γ may serve as biomarkers to distinguish HMPV from IVA and IVB, whereas IL-6 may be able to differentiate between HMPV and IVA. IL-6 and TNF-α may be used to assessment of severity and prognosis of HMPV infection.

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Section: Discussionmentioning

confidence: 99%

Profiles and Predictive Value of Cytokines in Children with Human Metapneumovirus Pneumonia

Xiang

Lin

Wang

et al. 2022

Preprint

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“…50) In a Peruvian study (2009-2010), M. pneumoniae was the most common etiologic agent of CAP, followed C. pneumoniae, and the most frequent respiratory viruses detected were RSV, IFV, and parainfluenza virus (PIV). 51) In an Australian case-control study of pneumonia (2015-2017), S. pneumoniae, H. influenzae, and Moraxella catarrhalis were detected at similar frequencies in children with versus without pneumonia. However, high vaccine coverage has almost eliminated pathogenic vaccine-type strains of S. pneumoniae and H. influenzae from this population.…”

Section: Worldwide Studies Of Causative Pathogens Of Capmentioning

confidence: 98%

Epidemiology and surveillance implications of community-acquired pneumonia in children

2022

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Community-acquired pneumonia (CAP) is the single largest infectious cause of hospitalization and death in children worldwide. With improved immunizations, the incidence of bacterial pneumonia and the number of colonized bacteria have decreased. However, respiratory viruses are still an important cause of CAP, especially as new infectious agents such severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerge. The SARS-CoV-2 virus emerged in 2019 and caused the current coronavirus disease 2019 pandemic. Therefore, it is necessary to elucidate the epidemiology and causative pathogens of CAP. Recently, the Pneumonia and Respiratory Disease Study Group, affiliated with the Korean Academy of Pediatric Allergy and Respiratory Disease, investigated the causative pathogens of respiratory infections in children hospitalized with CAP, the serotype of Streptococcus pneumoniae, and the prevalence of Mycoplasma pneumoniae with gene mutations. Antibiotic resistance and serotype test results can determine the use of empirical antibiotics. Moreover, it is possible to help develop future vaccines by comparing bacterial culture results with vaccine serotype and identifying the changes and prevalence of each serotype. Therefore, we will perform continuous national surveillance and monitor the epidemiology of respiratory pathogens in Korea and worldwide. The surveillance of these respiratory infections can play a role in monitoring the emergence of new infectious diseases such as SARS-CoV-2.

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“…ARIs are mainly caused by a wide range of viruses and bacteria [5,6]. ARIs and can be detected in more than 40% of patients [2,[11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Identification of viral and bacterial etiologic agents of the Pertussis-like syndrome in children under 5 years old hospitalized

Macedo¹

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Objective: To study the presence of 8 respiratory viruses (Influenza-A, Influenza-B, RSV-A, RSV-B, Adenovirus, Parainfluenza-1, Parainfluenza-2 and Parainfluenza-3) and atypical bacteria (Mycoplasma pneumonia and Chlamydia pneumonia) in samples from Peruvian children under 5 years-old previously analyzed for Bordetella. Pertussis. Methods: A secondary data analysis was performed from a previous cross-sectional study conducted in children with a probable diagnosis of Pertussis from January 2010 to July 2012. All samples were analyzed via Polymerase Chain Reaction (PCR) for the follow ing

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Detection of human Metapneumovirus infection in children under 18 years old hospitalized in Lima-Peru

Cited by 4 publications

References 60 publications

Profiles and Predictive Value of Cytokines in Children with Human Metapneumovirus Pneumonia

Profiles and Predictive Value of Cytokines in Children with Human Metapneumovirus Pneumonia

Epidemiology and surveillance implications of community-acquired pneumonia in children

Identification of viral and bacterial etiologic agents of the Pertussis-like syndrome in children under 5 years old hospitalized

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