Detection of Human Herpesvirus 8 DNA in Kaposi's Sarcoma Lesions and Peripheral Blood of Human Immunodeficiency Virus‐Positive Patients and Correlation with Serologic Measurements

Smith, Marilyn S.; Bloomer, Clark; Horvat, Rebecca T.; Goldstein, Elliot; Casparian, J. Michael; Chandran, Bala

doi:10.1086/514043

Cited by 96 publications

(68 citation statements)

References 26 publications

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“…Two of the 5 samples showed low anti-LNA antibody titers and were negative by both lytic IFA and PCR and the other 3 were positive by PCR or lytic antibodies. These HHV-8-positive/KS-negative patients will be followed up to check if the antibody titers are predictive of KS, as previously shown by other investigators (25,26).…”

Section: Discussionmentioning

confidence: 88%

HHV-8 infection in patients with AIDS-related Kaposi's sarcoma in Brazil

Keller

Zago

Viana

et al. 2001

Braz J Med Biol Res

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The aims of the present study were to determine the prevalence of human herpesvirus type 8 (HHV-8) in HIV-positive Brazilian patients with (HIV+/KS+) and without Kaposis sarcoma (HIV+/KS-) using PCR and immunofluorescence assays, to assess its association with KS disease, to evaluate the performance of these tests in detecting HHV-8 infection, and to investigate the association between anti-HHV-8 antibody titers, CD4 counts and staging of KS disease. Blood samples from 66 patients, 39 HIV+/KS+ and 27 HIV+/KS-, were analyzed for HHV-8 viremia in peripheral blood mononuclear cells by PCR and HHV-8 antigenemia for latent and lytic infection by immunofluorescence assay. Positive samples for latent nuclear HHV-8 antigen (LNA) antibodies were titrated out from 1/100 to 1/409,600 dilution. Clinical information was collected from medical records and risk behavior was assessed through an interview. HHV-8 DNA sequences were detected by PCR in 74.3% of KS+ patients and in 3.7% of KS-patients. Serological assays were similar in detecting anti-LNA antibodies and anti-lytic antigens in sera from KS+ patients (79.5%) and KS-patients (18.5%). HHV-8 was associated with KS whatever the method used, i.e., PCR (odds ratio (OR) = 7.4, 95% confidence interval (CI) = 2.16-25.61) or anti-LNA and anti-lytic antibodies (OR = 17.0, 95%CI = 4.91-59.14). Among KS+ patients, HHV-8 titration levels correlated positively with CD4 counts (rho 0.48, P = 0.02), but not with KS staging. HHV-8 is involved in the development of KS in different geographic areas worldwide, as it is in Brazil, where HHV-8 is more frequent among HIV+ patients. KS severity was associated with immunodeficiency, but no correlation was found between HHV-8 antibody titers and KS staging.

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Section: Discussionmentioning

confidence: 88%

HHV-8 infection in patients with AIDS-related Kaposi's sarcoma in Brazil

Keller

Zago

Viana

et al. 2001

Braz J Med Biol Res

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“…Previous studies have shown that the detection of HHV-8 antibodies for lytic proteins was generally more sensitive than the detection of HHV-8 DNA in peripheral blood mononuclear cells for the assessment of HHV-8 infections in HIVinfected patients with KS (13,33). By contrast, some authors (15,28) have found HHV-8 DNA by PCR in bone marrow stromal cells obtained from patients with multiple myeloma in whom HHV-8-specific antibodies had not been detected.…”

Section: Vol 42 2004 Serological Methods For Detection Of Hhv-8 Antmentioning

confidence: 98%

“…Many serologic tests have been developed for detection of HHV-8-specific antibodies (25,31,33). An enzyme-linked immunosorbent assay (ELISA) that uses sucrose-purified whole virus derived from the KS-1 cell line is now commercially available from Advanced Biotechnologies Inc. (ABI).…”

mentioning

confidence: 99%

“…Many cell lines latently infected by HHV-8 are commonly used as substrate cells for IFAs. HHV-8 lytic antigens can also be detected by IFAs following chemical induction of HHV-8-positive lymphoma cell lines with phorbol ester or sodium butyrate (19,31,33). However, there is imperfect correlation between all serological methods, and none can detect specific HHV-8-specific antibodies in all KS cases.…”

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confidence: 99%

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Comparative Evaluation of Three Serological Methods for Detection of Human Herpesvirus 8-Specific Antibodies in Canadian Allogeneic Stem Cell Transplant Recipients

et al. 2004

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“…Another study, using small viral capsid antigen (sVCA) found 3 of 28 (11%) US blood donors to be seropositive for HHV-8, although none of 25 hemophiliac patients or 22 children (10 HIV-negative and 12 HIV-positive) were HHV-8 seropositive 43 . In another study, none of the 52 patients tested who were negative for HIV and hepatitis B and C virus had antibodies for HHV-8 44 . In addition, using a latencyassociated nuclear antigen (LANA) IFA and an ELISA with plates coated with capsid-related protein encoded by ORF 65, 24.1% of 779 Italian blood donors from different regions had antibodies to at least 1 antigen 45 .…”

Section: Epidemiology Of Hhv-8 Infection -Serological Datamentioning

confidence: 94%

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

Leão¹,

Caterino–de–Araujo

Porter

et al. 2002

Rev. Hosp. Clin.

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OBJECTIVE: To review the current literature on human herpesvirus 8 with particular attention to the aspects related to the etiopathogenesis of Kaposi's sarcoma. MATERIALS AND METHODS: The authors searched original research and review articles on specific aspects of human herpesvirus 8 infection, including virology, epidemiology, transmission, diagnosis, natural history, therapy, and Kaposi's sarcoma etiopathogenesis. The relevant material was evaluated and reviewed. RESULTS: Human herpesvirus 8 is a recently discovered DNA virus that is present throughout the world but with major geographic variation. In the Western world, the virus, transmitted mainly by means of sexual contact, is strongly associated with Kaposi's sarcoma and body cavity-based lymphoma and more controversially with multiple myeloma and other non-proliferative disorders. There is no specific effective treatment, but HIV protease inhibitors may play an indirect role in the clearance of human herpesvirus 8 DNA from peripheral blood mononuclear cells of HIV-infected patients. Human herpesvirus 8 DNA is present in saliva, but there are as yet no documented cases of nosocomial transmission to health care workers. The prevalence of human herpesvirus 8 among health care workers is probably similar to that in the general population. CONCLUSION: Human herpesvirus 8 appears to be, at least in Western Europe and United States, restricted to a population at risk of developing Kaposi's sarcoma. Human herpesvirus 8 certainly has the means to overcome cellular control and immune responses and thus predispose carriers to malignancy, particularly Kaposi's sarcoma. The wide diffusion of Human herpesvirus 8 in classic Kaposi's sarcoma areas appears to represent an important factor in the high incidence of the disease. However, additional co-factors are likely to play a role in the development of Kaposi's sarcoma.

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Detection of Human Herpesvirus 8 DNA in Kaposi's Sarcoma Lesions and Peripheral Blood of Human Immunodeficiency Virus‐Positive Patients and Correlation with Serologic Measurements

Cited by 96 publications

References 26 publications

HHV-8 infection in patients with AIDS-related Kaposi's sarcoma in Brazil

HHV-8 infection in patients with AIDS-related Kaposi's sarcoma in Brazil

Comparative Evaluation of Three Serological Methods for Detection of Human Herpesvirus 8-Specific Antibodies in Canadian Allogeneic Stem Cell Transplant Recipients

Human herpesvirus 8 (HHV-8) and the etiopathogenesis of Kaposi's sarcoma

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