Detection of Human Gastric Cancer in Resected Specimens Using a Novel Infrared Fluorescent Anti-Human Carcinoembryonic Antigen Antibody with an Infrared Fluorescence Endoscope in Vitro

Abstract

An anti-CEA antibody with affinity for cancerous lesions and labeled with ICG-sulfo-OSu can therefore be imaged using this infrared fluorescence endoscope. Specific antibodies tagged with ICG-sulfo-OSu can label cancer cells and can generate a strong enough fluorescent signal to detect small cancers when examined with an infrared fluorescence endoscope.

Shedding light onto live molecular targets

“…For a review of intravital microscopy see several excellent recent review articles [18,20,21]. Macroscopic fluorescence reflectance imaging (FRI) is a useful technique when probing superficial structures (<5 mm deep), for example during endoscopy [22,23], dermatological imaging [3], intraoperative imaging [24], probing tissue autofluorescence [25,26] or small animal imaging [27]. Fluorescence molecular tomography is the newest imaging technology that has recently been shown to localize and quantify fluorescent probes threedimensionally in deep tissues at high sensitivities [28,29] (Fig.…”

Protease sensors for bioimaging

“…The feasibility and efficacy of fluorescence endoscopy has already been demonstrated in animal experiments as well as in human studies [25,26]. Special imaging catheters have been designed to transmit NIR light and have also been miniaturized to submillimeter dimensions for endoscopic imaging in the mouse model [27].…”

Endoscopic Molecular Imaging in Gastrointestinal Oncology

“…The sample was treated with normal horse serum (blocking serum) for 15 min. The surface of the lesion and normal mucosa were then treated with ICG-derivative-labeled antibody for 60 min at room temperature (Ito et al, 2001). Then, normal mucosa and the cancerous area were compared using the infrared fluorescence endoscope.…”

“…Based on the results of immunofluorescence using infrared fluorescence microscopy, a prototype of infrared fluorescence endoscopy was developed to observe the human gastrointestinal tract (Figure 4) (Ito et al, 2001). The system consisted of an infrared endoscope (Olympus XGIF-Q40IR, Olympus) coupled with an image-capturing device.…”