Detection of Human Enteric Viruses in Freshwater from European Countries

D’Ugo, Emilio; Marcheggiani, Stefania; Fioramonti, Ilaria; Giuseppetti, Roberto; Spurio, Roberto; Helmi, Karim; Guillebault, Delphine; Medlin, Linda K.; Simeonovski, Ivan; Boots, Bas; Breitenbach, Ulrich; Köker, Latife; Albay, Meriç; Mancini, Laura

doi:10.1007/s12560-016-9238-4

Cited by 21 publications

(4 citation statements)

References 39 publications

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“…Samples 1B, M16, and MM represent superficial samples and were collected from just under the water surface, whereas sample 2B was collected near the bottom of the lake; such a sampling design was pursued in order to understand changes in the microbial community structure along the water column. Water samples were collected using 1-L Pyrex glass bottles and stored in ice (4 °C) boxes; samples were later processed using a previously described ultrafiltration protocol (D’Ugo et al 2016 ). The elutriate (50 mL) was used for downstream laboratory investigations.…”

Section: Methodsmentioning

confidence: 99%

Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem

D’Ugo¹,

Bruno²,

Mukherjee

et al. 2021

Environ Sci Pollut Res

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Microbiomes of freshwater basins intended for human use remain poorly studied, with very little known about the microbial response to in situ oil spills. Lake Pertusillo is an artificial freshwater reservoir in Basilicata, Italy, and serves as the primary source of drinking water for more than one and a half million people in the region. Notably, it is located in close proximity to one of the largest oil extraction plants in Europe. The lake suffered a major oil spill in 2017, where approximately 400 tons of crude oil spilled into the lake; importantly, the pollution event provided a rare opportunity to study how the lacustrine microbiome responds to petroleum hydrocarbon contamination. Water samples were collected from Lake Pertusillo 10 months prior to and 3 months after the accident. The presence of hydrocarbons was verified and the taxonomic and functional aspects of the lake microbiome were assessed. The analysis revealed specialized successional patterns of lake microbial communities that were potentially capable of degrading complex, recalcitrant hydrocarbons, including aromatic, chloroaromatic, nitroaromatic, and sulfur containing aromatic hydrocarbons. Our findings indicated that changes in the freshwater microbial community were associated with the oil pollution event, where microbial patterns identified in the lacustrine microbiome 3 months after the oil spill were representative of its hydrocarbonoclastic potential and may serve as effective proxies for lacustrine oil pollution.

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Section: Methodsmentioning

confidence: 99%

Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem

D’Ugo¹,

Bruno²,

Mukherjee

et al. 2021

Environ Sci Pollut Res

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“…The fluorescent probe was FAM-TGGACAGCCCACCACCGCAAGTGT-BHQ1. The qRT-PCR reaction conditions were set up based on a previously published detection method for EVs [32]. A standard curve was established: Y =−3.2995 X + 30.96, R 2 = 0.99, and the detection limit was 3.96 copies/µL.…”

Section: Methodsmentioning

confidence: 99%

A neonatal murine model of coxsackievirus A4 infection for evaluation of vaccines and antiviral drugs

Zhang

Carr

et al. 2019

Emerging Microbes & Infections

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Coxsackievirus A4 (CVA4) infection can cause hand, foot and mouth disease (HFMD), an epidemic illness affecting neonatal and paediatric cohorts, which can develop to severe neurological disease with high mortality. In this study, we established the first ICR mouse model of CVA4 infection for the evaluation of inactivated vaccines and antiviral drug screening. The CVA4 YT226R strain was selected to infect the neonatal mice and three infectious factors were optimized to establish the infection model. The 3-day-old neonatal mice exhibited clinical symptoms such as hind limb paralysis and death. The severe inflammatory reactions were closely related to the abnormal expression of the acute phase response proinflammatory cytokine IL-6 and an imbalance in the IFN-γ/IL-4 ratio. Importantly, the inactivated CVA4 whole-virus vaccine induced humoral immune responses in adult females and the maternal antibodies afforded mice complete protection against lethal dose challenges of homologous or heterologous CVA4 strains. Both IFN-α2a and antiserum inhibited the replication of CVA4 and increased the survival rates of neonatal mice during the early stages of infection. This neonatal murine model of CVA4 infection will be useful for the development of prophylactic and therapeutic vaccines and for screening of antiviral drugs targeting CVA4 to decrease morbidity and mortality.

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“…Different types of enteric viruses have been recovered from various environmental water matrices including freshwater habitat (19). These viruses are of public health importance, because of their link to infections such as gastroenteritis in communities from poor sanitation settings (20).…”

Section: Introductionmentioning

confidence: 99%

Effects of Contamination of Freshwater Habitat With Common Heavy Metals and Anions on the Prevalence of Human Adenoviruses and Enteroviruses

Opere

Maingi

Ombori

2021

Front. Public Health

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The occurrence and survival of enteric viruses in open surface waters can be impacted by a host of factors including fecal emission levels, seasonal variations, virus stability and the physicochemical parameters. In this research, we aimed to document the association between contaminations of water samples with human enteric viruses (adenoviruses and enteroviruses) from a freshwater lake with variations in chemical contaminants. We collected 216 water samples from October 2010 to April 2012, from a 4 km stretch along Lake Victoria (LV) basin in Homa Bay town located in the western region of Kenya. The samples were analyzed for the existence of human adenoviruses (HAdV) and human enteroviruses (HEV), using the nested PCR (nPCR). We also assessed in the water samples the levels of twelve chemical contaminants consisting of six heavy metal elements and six anions. About 8.3 % of the samples were found to be contaminated with the enteric viruses. The concentrations of the 12 chemical contaminants were found to be largely within the WHO suggested limits. Most of the chemical contaminants were not related to the detection rates of the viruses from the statistical analysis. However, some positive and negative associations between the viral genome's detection and the chemical concentrations were established for only three metals (Fe, Pb, Cd) and the PO43− Radical. Cd had a weak positive significant relationship with HAdV (rho = 0.146, p = 0.032) while Pb and Fe had a weak positive significant relationship with HEV genome detection (rho = 0.156, p = 0.022) and (rho = 0.148 and p = 0.029) respectively. There was a modest negative relationship between phosphate ions and HEV (rho = −0.174, p = 0.010). The results of our study do not provide support for the hypothesis of an association between the presence of human enteric viruses and the levels of twelve chemical contaminants.

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Detection of Human Enteric Viruses in Freshwater from European Countries

Cited by 21 publications

References 39 publications

Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem

Characterization of microbial response to petroleum hydrocarbon contamination in a lacustrine ecosystem

A neonatal murine model of coxsackievirus A4 infection for evaluation of vaccines and antiviral drugs

Effects of Contamination of Freshwater Habitat With Common Heavy Metals and Anions on the Prevalence of Human Adenoviruses and Enteroviruses

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