Detection of human cytomegalovirus‐specific iga antibodies in colostrum by enzyme‐linked lmmunosorbent assay (elisa)

Pinku, Avraham; Haikin, Hava; Friedman, Maureen G.; Sarov, Israel

doi:10.1002/jmv.1890090205

Cited by 8 publications

(2 citation statements)

References 20 publications

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“…Perinatal CMV infections were shown to be either asymptomatic or associated with respiratory syndromes, pneumonitis or hepatitis [Andersen et al, 1972;Stern, 1975;Granstrom et al, 1977;Ahlfors et al, 1978;Stagno et al, 1981;Panjvani and Hanshaw, 19811. CMV excreted in the endocervix, breast milk, and saliva were suggested to be the source of perinatal CMV infections and virus perpetuation [Hayes et al, 1972: Numazaki et al, 1970Reynolds et al, 1973: Granstriim et al, 1977Cabau et al, 1979;Stagno et al, 19811. Recently, in this laboratory, Pinku et al [1982] have demonstrated specific CMVIgA antibodies in colostrum of 60% of CMV seropositive women by enzyme-linked immunosorbent assay (ELISA). Both congenitally and perinatally infected children excrete CMV in the urine and nasopharynx for several months or even years despite high levels of' circulating antibody, and serve as a constant reservoir for horizontal spread of infection [Hanshaw et al, 1965: Lang, 1975Cabau et al, 19791.…”

Section: Introductionmentioning

confidence: 98%

Prevalence of antibodies to human cytomegalovirus in Urban, Kibbutz, and bedouin children in southern Israel

Sarov¹,

Naggan²,

Rosenzveig³

et al. 1982

Journal of Medical Virology

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Prevalence of antibody to human cytomegalovirus (CMV) was determined in a sample of 860 healthy children aged 1-13 years in Beer Sheva, in the Negev region of Israel. Three groups of children were tested: (1) urban Jewish children of low, middle, and high socioeconomic levels; (2) 8 rural communes (kibbutz) in which children live in close contact with each other from the 6th week of life under good hygienic conditions and a high standard of living; (3) Bedouin, seminomadic Arabs living in relatively poor hygienic conditions in the desert. Kibbutz children showed a significantly higher rate of CMV seropositivity by the second year of life than urban Jewish and Bedouin children (76% versus 44% and 54% respectively) with a gradual increase to 94% in the 10-13-year age group. Among children living in urban populations a significantly higher prevalence of CMV seropositivity was associated with crowding, but not with other socioeconomic indicators (place of residence, country of origin, or education level of parents). A marked rise of CMV seropositivity with age was found in urban Jewish children in the 2-5-year age group during which time they attend nurseries (44% to 67%) and in the Bedouin children in the 6-9-year age group (59% to 86%) when they first attend school. The data suggest that close contact is of major importance in CMV infection in childhood. The clinical implications of early acquisition and high prevalence of CMV antibodies in the kibbutz setting are discussed.

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Section: Introductionmentioning

confidence: 98%

Prevalence of antibodies to human cytomegalovirus in Urban, Kibbutz, and bedouin children in southern Israel

Sarov¹,

Naggan²,

Rosenzveig³

et al. 1982

Journal of Medical Virology

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“…Specific IgA antibodies to microbial antigens have been reported in a few cases only, e.g., to pili of Klebsiella pneumoniae (5); to Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, poliovirus, coxsackie B virus, echovirus, and influenza virus (8); to Mycobacterium leprae (19), and to cytomegalovirus (20). The exciting role of IgA antibodies to peptidoglycan is based on the fact that peptidoglycan, with the exceptions of the mycoplasmatales and the archaebacteria (30), may be regarded a kind of common antigen among bacteria and that the R-D-Ala2 sequence of the peptide subunit represents a specific antigen of clinically relevant gram-positive cocci.…”

Section: Downloaded Frommentioning

confidence: 99%

Specific immunoglobulin A antibodies to a peptide subunit sequence of bacterial cell wall peptidoglycan

Franken¹,

Seidl²,

Kuchenbauer³

et al. 1984

Infect Immun

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Immunoglobulin A (IgA) antibodies in human sera with binding specificity for the C-terminal R-D-Ala-D-Ala sequence of the precursor peptide from bacterial cell wall peptidoglycan were detected by an enzymelinked immunosorbent assay (ELISA). Specificity of the test system was proved by comparing the high binding of specific IgA to albumin-(D-Ala3)9 as an antigen with the failure to bind to albumin-(L-Ala3)13, by binding inhibition studies with L-Ala3, D-Ala3, or peptides with structural analogy to peptidoglycan peptide subunit peptides as inhibitors, and by excluding binding of peroxidase-labeled anti-human IgA to immunoglobulin classes others than IgA. Interference of rheumatoid factors of the IgA class was excluded by an ELISA for assaying IgA-rheumatoid factor and by the fact that an IgA fraction essentially free of IgG and IgM was isolated from a serum reacting strongly positive in the ELISA for measuring specific IgA to the peptide subunit of peptidoglycan. This isolated IgA again exhibited binding specificity in the ELISA, thus corroborating the existence of specific IgA in human serum to the C-terminal R-D-Ala-D-Ala sequence of peptidoglycan precursor peptide. The existence of IgA antibodies with specificity for bacterial peptidoglycan was further proved by preadsorption of serum to peptidoglycans and subsequent measurement of specific IgA in the ELISA. Screening of human sera for IgA antibodies with specificity for R-D-Ala-D-Ala peptides revealed that specific antibodies directed against this sequence of bacterial cell wall peptidoglycan may be detected in several human sera.

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Detection of cytomegalovirus antigen and antibodies in the urine of small infants and children

Naqvi

Blair

1986

Journal of Medical Virology

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The diagnostic efficacy of an enzyme immune assay to detect cytomegalovirus (CMV) antigen in the urine of infected infants and children was determined, and the effect that CMV-specific antibodies present in the urine have on the sensitivity of the test was ascertained. The antigen was detected in 84.4% of the CMV-culture-positive samples, while CMV-specific IgA was detected in 24% of CMV-culture-positive specimens. The absence of CMV-specific IgA correlated with detection of CMV antigen in the CMV-culture-positive urine specimens (p less than 0.05).

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Detection of human cytomegalovirus‐specific iga antibodies in colostrum by enzyme‐linked lmmunosorbent assay (elisa)

Cited by 8 publications

References 20 publications

Prevalence of antibodies to human cytomegalovirus in Urban, Kibbutz, and bedouin children in southern Israel

Prevalence of antibodies to human cytomegalovirus in Urban, Kibbutz, and bedouin children in southern Israel

Specific immunoglobulin A antibodies to a peptide subunit sequence of bacterial cell wall peptidoglycan

Detection of cytomegalovirus antigen and antibodies in the urine of small infants and children

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