Detection Of Human Contact Sensitivity To Dinitrochlorobenzene By The Migration Inhibition Test

Nishioka, K

doi:10.1111/1523-1747.ep12482903

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…N ishioka [10] reported that the patient's peripheral lymphocytes following exposure to dinitrophenylated microsomes derived from both human and guinea pig epidermis, could elicit the release of M IF in the contact sensitivity to DNCB.…”

Section: Discussionmentioning

confidence: 99%

Drug-Induced Lymphocyte Transformation in Peripheral Lymphocytes from Patients with Drug Eruptions

Suzuki¹,

Asai²,

Hamada³

et al. 1978

Dermatology

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In some types of drug eruptions, a positive blastoid transformation is frequently seen in peripheral lymphocytes when they are stimulated in vitro with the causative drug in the presence of autologous serum. In these cases, a soluble fraction prepared from guinea pig skin can replace the autologous serum in inducing the lymphocyte transformation, whereas either the mitochondrial or microsomal fraction of guinea pig skin instead of the autologous serum is less effective. Moreover, in fixed drug eruption, the addition of soluble skin material fractionated by gel filtration with Sephadex G-100 (FrD₁ is as effective as the autologous sera in inducing lymphocyte transformation. These results suggest that the soluble fraction of skin is related to the pathogenesis of fixed and exanthematic drug eruptions as a carrier substance of drugs.

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Section: Discussionmentioning

confidence: 99%

Drug-Induced Lymphocyte Transformation in Peripheral Lymphocytes from Patients with Drug Eruptions

Suzuki¹,

Asai²,

Hamada³

et al. 1978

Dermatology

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“…The TNCB immune sera had no effect in suppressing DNFB con tact sensitivity in our previous study [7], It is difficult to explain this discrepancy at present. The assay sys tem used in this study correlates well with the skin reaction of contact sensitivity in guinea pigs as well as in humans [9,10]. However, MIF is not the only lymphokine working in the elicitation phase of contact sensitivity.…”

Section: Discussionmentioning

confidence: 93%

“…The antibody was introduced into the macrophage migration inhibitory factor (MIF) assay of contact sensitivity which has been proved to have good correlation with in vivo reaction of contact sensitivity [9,10],…”

Section: Introductionmentioning

confidence: 99%

Suppression of Contact Sensitivity by IgG1 Antihapten Antibody

Katayama¹,

Nishioka²

1983

Int Arch Allergy Immunol

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Sera obtained from guinea pigs sensitized with 2,4-dinitrofluorobenzene (DNFB) showed suppressive activity on the migration inhibitory factor (MIF) production by the lymph node cells obtained from DNFB-contact-sensitized guinea pigs. A similar suppressive effect was achieved by the sera of 2,4,6-trinitro-chlorobenzene- (TNCB-)sensitized guinea pigs. The suppressive factor in the sera was found to be IgG1 antihapten antibody. It suppressed MIF production dose-dependently during a period between 3 h before and 1 h after the antigen stimulation. Pretreatment of macrophages with IgG1 antihapten antibody abolished the MIF production of the lymph node cell. The antibody lost its suppressive activity after splitting off its Fc portion, and pretreatment of macrophages with Fc fragments of normal IgG1 interfered with the action of the IgG1 antibody. Therefore, the IgG1 antihapten antibody in the sera of contact-sensitized guinea pigs acted on macrophages through its Fc portion to inhibit MIF production by lymph node cells.

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“…Using the capil lary technique of S0borg and Bendixen [15], Ham ilton and Ledger [8] described in vitro reactivity of human cells after DNCB sensitization. Nlshioka [13] found significant MIF production in DNCB-sensitized patients; MIF tests were per formed on guinea pig peritoneal macrophages.…”

Section: Methodsmentioning

confidence: 97%

Two-Step Leukocyte Migration in Agarose Technique

Agrup¹,

Källén²,

Nilsson³

1980

Int Arch Allergy Immunol

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A two-step modification of Clausen’s technique of leukocyte migration under agarose is described. Blood leukocytes from a subject are incubated for approximately 4 days with or without antigen. The cultures, after reconstitution with antigen followed by repeated washing of the cultured cells, are added to fresh, dextran-separated blood leukocytes, and migration tests are performed in the absence of the antigen. Cells preincubated with antigen give a very marked reduction of the migration of the target leukocytes which must not necessarily be autologous. An inhibition of target cell migration can be seen with cells cultured without antigen for up to 3 days; with longer culture time, this effect is reduced or disappears. The effect of cells cultured with antigen, however, is equally strong irrespective of the culture time up to 1 week, and the most clearcut results will, therefore, be obtained after approximately 4 days culture. Also migration enhancement has been seen. Treatment of cells during the incubation with antigen and cycloheximide gives a dose-dependent blocking of the inhibiting effect. 5 patients were studied both before and after sensitization with DNCB: low concentrations of DNCB gave an inhibition and high concentrations an enhancement of the migration when the patients were sensitized. The technique is more sensitive and reproducible than the original Clausen technique. It is also suitable in studies where shortage of cells is a problem.

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Detection Of Human Contact Sensitivity To Dinitrochlorobenzene By The Migration Inhibition Test

Cited by 9 publications

References 22 publications

Drug-Induced Lymphocyte Transformation in Peripheral Lymphocytes from Patients with Drug Eruptions

Drug-Induced Lymphocyte Transformation in Peripheral Lymphocytes from Patients with Drug Eruptions

Suppression of Contact Sensitivity by IgG1 Antihapten Antibody

Two-Step Leukocyte Migration in Agarose Technique

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