Detection of HPV in oral leukoplakia by brushing and biopsy: prospective study in an Italian cohort

Vella, Fedora Della; Pannone, Giuseppe; Patano, Assunta; Ninivaggi, Rossella; Prete, Raffaele Del; Lauritano, D; Petruzzi, Massimo

doi:10.1007/s00784-019-03048-y

Cited by 8 publications

(14 citation statements)

References 29 publications

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“…The presence of HR‐HPV in OL differs significantly across published studies, possibly reflecting differences in sampling techniques and geographical regions. Some studies have suggested an association of OL with HR‐HPV (Chen & Zhao, 2017; Ferreira, Biasoli, Bernabe, Nunes, & Miyahara, 2017; Syrjanen et al., 2011), while other studies have reported the absence of HPV or its presence at very low rates (Bhargava et al., 2016; Della Vella et al., 2019; Yang et al., 2019), in agreement with our findings. Geographical variations in the prevalence of HPV infection and the importance of HPV for OL or oral cancer have been reported, and further investigations of these topics are required.…”

Section: Discussionsupporting

confidence: 92%

High‐risk human papillomavirus in patients with oral leukoplakia and oral squamous cell carcinoma—A multi‐centre study in Sweden, Brazil and Romania

et al. 2020

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Objectives Although causal associations between oral leukoplakia (OL), oral squamous cell carcinoma (OSCC) and high‐risk human papillomavirus (HR‐HPV) have been speculated upon in several reports, conclusive evidence has not been presented. This study investigates whether the number of cases of HR‐HPV in OL has increased over time and whether the prevalence of HR‐HPV‐positive OL differs in various parts of the world. Patients and Methods A total of 432 patients with OL from Sweden, Brazil and Romania were analysed. Patients were divided into historical (1992–2002) and contemporary (2011–2017) cohorts from the respective countries. Seventeen patients with OL developed oral squamous cell carcinoma (OSCC). A real‐time PCR assay, targeting HPV sub‐types 6,11,16,18,31,33,35,39,45,52,56,58 and 59, was performed to detect HR‐HPV in patients with OL. Results In the Swedish and Romanian cohorts, none of the investigated HPV sub‐types were detected. In the Brazilian cohorts, five patients with OL (3%) were positive for HR‐HPV, including four patients from the contemporary cohort (HPV 16, 31, 33) and one from the historical cohort (HPV 11). All the cases of OL that transformed into OSCC were HR‐HPV‐negative, as were the corresponding tumours. Conclusions In summary, the prevalence of HR‐HPV in OL is low in all the tested countries, and the incidence has not changed over time. HR‐HPV in OL does not seem to be a driver of oncogenesis.

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Section: Discussionsupporting

confidence: 92%

High‐risk human papillomavirus in patients with oral leukoplakia and oral squamous cell carcinoma—A multi‐centre study in Sweden, Brazil and Romania

et al. 2020

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“…The fact that brush samples from the oral cavity mucosa came from lesions obtained from various areas with different surface layers, some of which were keratinized, may explain the difficulties encountered in acquiring representative samples. Della Vella et al (49) found the HPV prevalence in incisional biopsies of oral leukoplakia to be 17% compared to 5% in brush biopsies analyzed with the same method as in our study. Their result differs significantly from ours as we found a level of agreement between the two DNA-methods to be 82.2% (k=0.54, p=0.001).…”

Section: Discussionsupporting

confidence: 58%

Brush Samples of Oral Lesions to FTA Elute Card for High-risk Human Papilloma Virus Diagnosis

Stark¹,

Gustavsson

Horal

et al. 2021

Anticancer Res

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“…It is likely prudent, however, to advise patients with OLK to refrain from using high alcohol‐containing mouthwashes (McCullough & Farah, 2008), given results of a large retrospective investigation which showed potential risks for head and neck cancer subsites and in long‐term and frequent users of mouthwash (Boffetta et al., 2016). HPV has not been found to be a substantial contributor to induction of OLK, with some studies failing to detect it in a wide variety of populations over a long timeframe (Sundberg et al, 2019; Sundberg et al, 2020), while others have shown only very low positive rates of detection (<5%) with no difference to normal or OSCC samples (Della Vella et al, 2020; Yang et al, 2019). Pooled estimates of HPV prevalence in OLK (20.2%) and PVL (24.7%) were no different to other potentially malignant conditions, such as oral lichen planus (23.0%) and oral submucous fibrosis (28.6%), and were independent of OED (de la Cour, Sperling, Belmonte, Syrjänen, & Kjaer, 2020).…”

Section: Clinical and Histopathological Determinants Of Malignant Tramentioning

confidence: 99%

Molecular, genomic and mutational landscape of oral leukoplakia

Farah

2020

Oral Diseases

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Oral leukoplakia (OLK) and its more aggressive clinical variant proliferative verrucous leukoplakia (PVL) remain enigmatic disorders clinically and histopathologically. Despite decades of research into both, there has been only incremental advancement in our understanding of their aetiology and pathogenesis and only minimal improvement in effective management strategies. Currently, no specific prognostic genetic or molecular marker has been reported for leukoplakia. There is, however, an emerging body of evidence characterising the genomic and transcriptomic profile of OLK. Regardless of the significance of cellular and architectural features of OLK and PVL, it is clear from studies reported in this review that new emerging evidence points to the presence of premalignant molecular subtypes of leukoplakia which require further investigation. This up‐to‐date review explores the contemporary genomic, transcriptomic and mutational landscape of leukoplakia broadly, discusses concepts that may not be widely recognised or accepted and purposefully highlights studies with juxtaposed findings in an effort to challenge dogma. It also highlights the urgent need for a concerted international effort of original collaborative research which will only occur by pooling collective efforts, resources and intellect to define the molecular fingerprint of this enigmatic disorder, in the hope it will better inform diagnosis, stratification and treatment.

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Detection of HPV in oral leukoplakia by brushing and biopsy: prospective study in an Italian cohort

Cited by 8 publications

References 29 publications

High‐risk human papillomavirus in patients with oral leukoplakia and oral squamous cell carcinoma—A multi‐centre study in Sweden, Brazil and Romania

High‐risk human papillomavirus in patients with oral leukoplakia and oral squamous cell carcinoma—A multi‐centre study in Sweden, Brazil and Romania

Brush Samples of Oral Lesions to FTA Elute Card for High-risk Human Papilloma Virus Diagnosis

Molecular, genomic and mutational landscape of oral leukoplakia

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