Detection of HPV in Japanese and Chinese oral carcinomas by in situ PCR

Uobe, Kenichi; Masuno, Koichi; Fang, Yu; Li, L.-J.; Wen, Yu-ming; Ueda, Yoshimichi; Tanaka, Akio

doi:10.1016/s1368-8375(00)00075-0

Cited by 51 publications

(59 citation statements)

References 21 publications

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“…Investigators showed a strong association between hig-risk-HPV infection and OSCC in Mexican, Sweden, Japanese, and Chinese populations (10)(11)(12). The role of high-risk oncogenic HPV in premalignant and malignant oral lesions has been an issue of extreme controversy with conflicting data reported by numerous studies.…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus in saliva of patients with oral squamous cell carcinoma

Sahebjamee¹,

Boorghani²,

Ghaffari³

et al. 2009

Med Oral

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Objective: The aim of this study was to evaluate the presence of human papillomavirus (HPV) in saliva rinses of patients with oral squamous cell carcinoma (OSCC)and to analyze the possibility of using saliva as a diagnostic method for screening high-risk patients. Study design: The saliva sample of 22 patients with oral squamous cell carcinoma and 20 age-sex matched healthy controls were obtained. The presence of HPV 6, 11, 16, 18, 31, and 33 was evaluated by polymerase chain reaction (PCR). Results: In 40.9% of the patients and in 25% of the controls, the saliva was shown to be positive for HPV. In 27.3% of the patients and in 20% of the controls, the saliva was shown to be positive for HPV16; and none of the controls, except one patient was shown to be positive for HPV 18. Neither patients nor controls were positive for HPV 31 and 33. These differences were not statistically significant. Conclusions: The results of this study were unable to support the detection of HPV in saliva rinses as a diagnostic method for OSCC.

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Section: Discussionmentioning

confidence: 99%

Human papillomavirus in saliva of patients with oral squamous cell carcinoma

Sahebjamee¹,

Boorghani²,

Ghaffari³

et al. 2009

Med Oral

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“…6 Besides the relevance of studies on several markers, it was mandatory to investigate the risk factors of continued use of tobacco, alcohol misuse, diet and viral infections. 7 In particular, it has been generally hypothesized that HPV infection may play a role in the occurrence of potentially malignant oral lesions and/or in their malignant transformation, [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] even if the detection rate of HPV DNA reported in carcinomas of the oral cavity is very discordant. 19 A previous study, carried out in our geographic area (southern Italy), determined that HPV prevalence was 61.5% in carcinomas, 27.1% in potentially malignant lesions, 26.5% in erosive ulcerative lesions and 5.5% in controls.…”

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confidence: 99%

Expression of cell cycle markers and human papillomavirus infection in oral squamous cell carcinoma: Use of fuzzy neural networks

Muzio

D’Angelo

Procaccini

et al. 2005

Intl Journal of Cancer

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Our aim was to evaluate in oral squamous cell carcinoma (OSCC) the relationship between some cell cycle markers and HPV infection, conditionally to age, gender and certain habits of patients, and to assess the ability of fuzzy neural networks (FNNs) in building up an adequate predictive model based on logic inference rules. Eighteen cases of OSCC were examined by immunohistochemistry for MIB-1, PCNA and survivin expression; presence of HPV DNA was investigated in exfoliated oral mucosa cells by nested PCR (nPCR, MY09-MY11/GP5-GP6), and HPV genotype was determined by direct DNA sequencing. Data were analyzed by traditional statistics (TS) and FNNs. HPV DNA was found in 9/18 OSCCs (50.0 %) without any significant higher risk of HPV infection with respect to the sociodemographic variables considered ( p > 0.2), apart from tobacco smoking, reported in 44.4% of OSCC HPV-positive vs. 100% HPV-negative subjects ( p = 0.029). Regarding cell cycle markers, TS and FNN revealed that survivin was expressed significantly more in HPV-negative than in HPVpositive OSCC [root mean-square error (RMSE) = 5.89 3 10 -6 , % predicted 100.0]; furthermore, smoking played a protective role for survivin expression in HPV-positive cases (OR = 0.019, 95%CI 0.001-0.723, RMSE = 0.20, % of prevision 94.4). FNN, although on a small sample size, allowed us to confirm data by TS and to hypothesize a different cell cycle pattern for HPV-positive vs. HPV-negative OSCC. In the latter cases, the relevance of apoptotic vs. proliferative markers suggested that they may be related to the different supposed outcome of HPV-negative OSCC and that HPV may have a protective role in the expression level of survivin, especially in tobacco smokers. ' 2005 Wiley-Liss, Inc.Key words: human papillomavirus; survivin; oral squamous cell carcinoma; fuzzy neural network; carcinogenesis OSCC is rapidly increasing in incidence and represents the most frequent malignant oral tumor. The incidence of metastasis depends on the degree of cellular differentiation, deep invasion and site of the primary tumor; however, outcome is difficult to predict if only standard clinicopathologic parameters are taken into account. Biologic markers that can help to identify lesions with an aggressive phenotype and worse prognosis need to be identified.Since the majority of human neoplasms are characterized by an imbalance of the regulatory cell cycle control processes, the study of OSCC using the expression of proteins involved in the critical checkpoints of cell apoptosis and growth starts by elucidating the processes of carcinogenesis and appears to have good prognostic value. 1 Indeed, we know that apoptosis, or programmed cell death, and its suppression are involved in the carcinogenesis of several tumors; this process, mediated by caspases and cysteine proteinases present as proenzymes, is inhibited by several proteins, such as those of the Bcl-2 and IAP families. Survivin is an IAP protein, which is abundantly expressed in most solid and hematologic malignancies but undetectable in ...

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“…In fact, two teams recently proposed two methods for HPV detection: in situ PCR and in situ PCR ISH. These methods combine the sensitivity of solution PCR with the subcellular localization provided by traditional ISH (Koyama et al, 2007;Uobe et al, 2001). Nevertheless, there are currently insufficient data in the literature to support the adequacy of these techniques.…”

Section: Incidence In Oral Tumorsmentioning

confidence: 99%

Human Papillomavirus and Related Diseases - From Bench to Bedside - A Clinical Perspective

Broeck¹

2012

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Detection of HPV in Japanese and Chinese oral carcinomas by in situ PCR

Cited by 51 publications

References 21 publications

Human papillomavirus in saliva of patients with oral squamous cell carcinoma

Human papillomavirus in saliva of patients with oral squamous cell carcinoma

Expression of cell cycle markers and human papillomavirus infection in oral squamous cell carcinoma: Use of fuzzy neural networks

Human Papillomavirus and Related Diseases - From Bench to Bedside - A Clinical Perspective

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