Detection of homology to the beta bacteriophage integration site in a wide variety of Corynebacterium spp

Cianciotto, Nicholas P.; Rappuoli, Rino; Groman, Neal B.

doi:10.1128/jb.168.1.103-108.1986

Cited by 20 publications

(14 citation statements)

References 16 publications

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“…Moreover, some strains of C. ulcerans and C. pseudotuberculosis can produce diphtheria toxin (DT). Furthermore, some non-toxigenic strains are converted to toxigeny (DT production) by β-phages from toxinogenic C. diphtheriae [15,23,24,44].…”

Section: Taxonomymentioning

confidence: 99%

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

et al. 2006

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-Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis (CLA), a common disease in small ruminant populations throughout the world. Once established, this disease is difficult to eradicate because drug therapy is not effective and because the clinical detection of infected animals is of limited efficiency. We reviewed the microbiological, biochemical and taxonomic features of C. pseudotuberculosis, general aspects of infection, the main virulence determinants and currently available commercial vaccines. We also examined the current molecular strategies for the study of virulence in C. pseudotuberculosis, including the latest research on the identification of novel virulence factors and genes, which will help us to better understand the biology of this microorganism. This knowledge may also contribute to the development of improved CLA vaccines, including subunit and DNA-based types, as well as to improve the diagnosis, treatment and control of this disease.

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Section: Taxonomymentioning

confidence: 99%

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

et al. 2006

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“…More specifically, each gene encoded a recombinase protein comprising 408 amino acids, with 11 (highly conservative) amino acid differences over their total lengths. The attB and attP regions from NCTC13129 and β are identical over the first 50 bp and in total share a 92 bp common 'core' region (Cianciotto et al, 1986): in addition, the core regions of β attP and the NCTC13129 prophage attP (deduced from attL1 and attR1) were identical over this region.…”

Section: Corynephage β Integration Functions and Construction Of Pkmo3wmentioning

confidence: 86%

“…The β attB site of C. diphtheriae is present in other Coryneform species (Cianciotto et al, 1986), opening up the possibility that the vectors developed here could be used to transform other species of Corynebacterium. To test this hypothesis we focused on two species: C. glutamicum and C. ulcerans.…”

Section: Use Of the Integration Vectors In Other Species Of Corynebacmentioning

confidence: 99%

“…For C. ulcerans 712, formation of lysogens with the phage sequences inserted at two different chromosomal sites (attB1 and attB2) following infection with β has been described (Cianciotto et al, 1986). The genome sequence was not available, although the sequence of attB1, but not attB2 had been determined previously (Cianciotto et al, 1990).…”

Section: Use Of the Integration Vectors In Other Species Of Corynebacmentioning

confidence: 99%

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Bacteriophage-based vectors for site-specific insertion of DNA in the chromosome of Corynebacteria

Oram

Woolston

Jacobson

et al. 2007

Gene

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In Corynebacterium diphtheriae, diphtheria toxin is encoded by the tox gene of some temperate corynephages such as β. β-like corynephages are capable of inserting into the C. diphtheriae chromosome at two specific sites, attB1 and attB2. Transcription of the phage-encoded tox gene, and many chromosomally-encoded genes, is regulated by the DtxR protein in response to Fe 2+ levels. Characterizing DtxR-dependent gene regulation is pivotal in understanding diphtheria pathogenesis and mechanisms of iron-dependent gene expression; although this has been hampered by a lack of molecular genetic tools in C. diphtheriae and related Coryneform species. To expand the systems for genetic manipulation of C. diphtheriae, we constructed plasmid vectors capable of integrating into the chromosome. These plasmids contain the β-encoded attP site and the DIP0182 integrase gene of C. diphtheriae NCTC13129. When these vectors were delivered to the cytoplasm of nonlysogenic C. diphtheriae, they integrated into either the attB1 or attB2 sites with comparable frequency. Lysogens were also transformed with these vectors, by virtue of the second attB site. An integrated vector carrying an intact dtxR gene complemented the mutant phenotypes of a C. diphtheriae ΔdtxR strain. Additionally, strains of β-susceptible C. ulcerans, and C. glutamicum, a species non-permissive for β, were each transformed with these vectors. This work significantly extends the tools available for targeted transformation of both pathogenic and non-pathogenic Corynebacterium species.

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“…These attB sites share a 96-bp sequence with the attP sites of ␤-related phages (37). Moreover, screening of a variety of Corynebacterium species including the soil isolate C. glutamicum ATCC 13032 with attP and attB probes showed that all of the species investigated contained at least one DNA fragment that hybridized with both of these probes (8,9,15). Besides this report, information on the molecular biology of lysogeny in coryneform bacteria remained scarce.…”

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confidence: 99%

Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus" C70

et al. 1996

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All the essential genetic determinants for site-specific integration of corynephage AAU2 are contained within a 1,756-bp DNA fragment, carried on the integrative plasmid p5510, and are shown to be functional in Escherichia coli. One open reading frame, ORF4, encoding a protein of 266 amino acids was shown to represent the AAU2 integrase. The nucleotide sequence of the AAU2 attachment site, attP, and the attB, attL, and attR sequences in the host ''Arthrobacter aureus'' C70 were determined. Identical nucleotide sequences were shown to be responsible for the integration of p5510 in the chromosomes of Corynebacterium glutamicum, Brevibacterium divaricatum, and B. lactofermentum, and a sequence almost identical to attB was found to be present in these three strains. In contrast to other phage site-specific recombination systems, a plasmid encompassing only int-attP failed to integrate into the host chromosome. This led to the identification of an 800-bp noncoding region, immediately upstream of int, absolutely required for site-specific integration of p5510.Genera Arthrobacter, Corynebacterium, and Brevibacterium belong to the group of coryneform bacteria from which several strains are industrially used for the production of certain amino acids (52). Most of the corynephages isolated to date originated from fermentation failures or soils and are virulent (19,45,47,51). AAU2 is a temperate phage isolated from soil that infects and lysogenizes ''Arthrobacter aureus '' C70 (24). Other known temperate corynephages have been isolated following UV or mitomycin induction of coryneform strains (20,29,30,34,42). Thus, a significant proportion of strains were shown to harbor prophages, but only for a few of them was the phage-host relationship studied (30, 42). The only well-documented temperate corynephage is phage ␤ of the human pathogen Corynebacterium diphtheriae (18). Its integration occurred into either of two sites (attB1 and attB2) located on the C. diphtheriae chromosome (36). These attB sites share a 96-bp sequence with the attP sites of ␤-related phages (37). Moreover, screening of a variety of Corynebacterium species including the soil isolate C. glutamicum ATCC 13032 with attP and attB probes showed that all of the species investigated contained at least one DNA fragment that hybridized with both of these probes (8,9,15). Besides this report, information on the molecular biology of lysogeny in coryneform bacteria remained scarce.The present study reports on the phage-encoded site-specific recombination system of AAU2, which has been pinpointed previously to a 5.25-kb BglII-EcoRV fragment on the phage genome (24). The phage attachment sites (attP, attB, attR, and attL) were sequenced, and a common sequence was identified. The AAU2 integrase gene was also delineated by transposon mutagenesis, and its deduced protein sequence was shown to resemble the conserved C-terminal region of other members of the integrase family. MATERIALS AND METHODSBacteriophage, bacterial strains, and plasmids. The bacteriophage, bacteria...

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Detection of homology to the beta bacteriophage integration site in a wide variety of Corynebacterium spp

Cited by 20 publications

References 16 publications

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

Bacteriophage-based vectors for site-specific insertion of DNA in the chromosome of Corynebacteria

Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus" C70

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