Detection of Homocysteine and Cysteine

Wang, Weihua; Rusin, Oleksandr; Xu, Xiangyang; Kim, Kyu Kwang; Escobedo, Jorge O.; Fakayode, Sayo O.; Fletcher, Kristin A.; Lowry, Mark; Schowalter, Corin M.; Lawrence, Candace M.; Fronczek, Frank R.; Warner, Isiah M.; Strongin, Robert M.

doi:10.1021/ja054962n

Cited by 561 publications

(273 citation statements)

References 63 publications

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“…In recent decades, many studies have demonstrated that metabolic disorder of biothiols will lead to extreme damage in human and numerous other animals (Townsend et al, 2003;Reddie and Carroll, 2008;Weerapana et al, 2010). Specifically, a low level of biothiols in vivo may cause slowed growth, liver damage, weight loss, and some other adverse influences (Wang et al, 2005;Duan et al, 2008;Yang et al, 2012), while a high level of biothiols is closely related to Parkinson's, Alzheimer's, and cardiovascular diseases (Heafield et al, 1990;Rusin et al, 2004;Lin et al, 2008;Yang et al, 2011). Thus, a lot of effort has been put into quantifying the lowmolecule-weight biothiols in serum (Ni et al, 2015;Ghasemi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Detection of biological thiols based on a colorimetric method

Sun

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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Abstract:Biological thiols (biothiols), an important kind of functional biomolecules, such as cysteine (Cys) and glutathione (GSH), play vital roles in maintaining the stability of the intracellular environment. In past decades, studies have demonstrated that metabolic disorder of biothiols is related to many serious disease processes and will lead to extreme damage in human and numerous animals. We carried out a series of experiments to detect biothiols in biosamples, including bovine plasma and cell lysates of seven different cell lines based on a simple colorimetric method. In a typical test, the color of the test solution could gradually change from blue to colorless after the addition of biothiols. Based on the color change displayed, experimental results reveal that the percentage of biothiols in the embryonic fibroblast cell line is significantly higher than those in the other six cell lines, which provides the basis for the following biothiols-related study.

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Section: Introductionmentioning

confidence: 99%

Detection of biological thiols based on a colorimetric method

Sun

Zhang

et al. 2016

J. Zhejiang Univ. Sci. B

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“…It is also widely used in the food industry as an antioxidant and in the pharmaceutical industry in drug formulation [6][7][8][9][10][11][12]. Therefore, the rapid, sensitive and selective detection of CysH are very important for investigating their functions in cells and disease diagnosis [13,14].…”

Section: Introductionmentioning

confidence: 99%

“…Most of them are based on the chromatographic separation [15,16], electrophoretic methods [17,18] and UV/vis absorption spectrometry [14,19,20]. Separation methods present basic limitations in terms of equipment cost, complexity, sample processing and run times.…”

Section: Introductionmentioning

confidence: 99%

The Electrochemical Behavior of Au/AuNPs/PNA/ZnSe-QD/ACA Electrode Towards CySH Oxidation

et al. 2015

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This work describes the electrochemical behavior of azodicarboxamide (ACA) film immobilized on the surface of penicillamine (PNA)/ZnSe-quantum dot (ZnSe-QD) gold nanoparticle (AuNPs) Au electrode. Electrocatalytic activity of modified electrode toward the oxidation of cysteine (CySH) was investigated. The surface structure and composition of the sensor were characterized by scanning electron microscopy (SEM). Oxidation of CySH on the surface of modified electrode was investigated with cyclic voltammetry, electrochemical impedance spectroscopy (EIS), hydrodynamic voltammetry and chronoamperometry methods. The results show that the PNA/ZnSe-QD/ACA film displays excellent electrochemical catalytic activities towards CySH oxidation. The modified electrode shows reproducible behavior and high level of stability during the electrochemical experiments. Also it has short response time, low detection limit, high sensitivity and low operation potential, which can be used as an amperometric sensor for monitoring of CySH. The proposed modified electrode was successfully used for determination of CySH in real sample such as human serum.

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“…[1][2][3] Cys provides a modality for the intramolecular crosslinking of proteins through disulfide bonds to support their secondary structures and functions. It is also a precursor of coenzyme A and can easily be oxidized to cystine which serves as a model for the thiol group of proteins in a variety of biological media.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Naked Eye Dual-readout Method for ʟ-Cysteine Detection Based on the NSET of Fluorophore Functionalized Gold Nanoparticles

Fu¹,

Liu²,

Wu³

et al. 2014

Bulletin of the Korean Chemical Society

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A simple, highly sensitive and selective method based on the rhodamine B-covered gold nanoparticle with dual-readout (colorimetric and fluorometric) detection for L-cysteine is proposed. A mechanism is that citratestabilized AuNPs were modified with RB by electrostatic interaction, which enables the nanometal surface energy transfer (NSET) from the RB to the AuNPs, quenching the fluorescence. In the presence of L-cysteine, it was used as a competitor in the NSET by the strongly Au-S bonding to release RB from the Au surface and recover the fluorescence, and the red-to-purple color change quickly, which was monitored simply by the naked eye. Under the optimum conditions, the detection limit is as low as 10 nM. The method possessed the advantages of simplicity, rapidity and sensitivity at the same time. The method was also successfully applied to the determination of L-cysteine in human urine samples, and the results were satisfying.

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Detection of Homocysteine and Cysteine

Cited by 561 publications

References 63 publications

Detection of biological thiols based on a colorimetric method

Detection of biological thiols based on a colorimetric method

The Electrochemical Behavior of Au/AuNPs/PNA/ZnSe-QD/ACA Electrode Towards CySH Oxidation

Highly Sensitive and Naked Eye Dual-readout Method for ʟ-Cysteine Detection Based on the NSET of Fluorophore Functionalized Gold Nanoparticles

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