1979
DOI: 10.1267/ahc.12.292
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Detection of Histamine in Rat Mast Cell Granules by Orcein-Water Blue Stain

Abstract: A new method of staining histamine in rat mast cell granules in formalin fixed paraffin section was developed.Mast cells of several organs of formalin fixed paraffin sections were stained well in red with orcein-water blue as well as in purple with toluidine blue.But only mast cells in dermis showed a different appearance in orcein-water blue stain and toluidine blue stain. The results of an electrophoretic study, diamine oxidase treatment and compound 48/80 treatment of rat lead to the conclusion that the sub… Show more

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“…Histamine in MCs was detected in the sections stained with solution of Water Blue -Orcein [19]. The results of cytochemical reaction were expressed as a percentage of positive cells [20].…”
Section: Methodsmentioning
confidence: 99%
“…Histamine in MCs was detected in the sections stained with solution of Water Blue -Orcein [19]. The results of cytochemical reaction were expressed as a percentage of positive cells [20].…”
Section: Methodsmentioning
confidence: 99%