Detection of high level aminoglycoside resistance genes among clinical isolates of Enterococcus species

Diab, Manal; Salem, Dalia; El‐Shenawy, A. I.; El-Far, Amira; Abdelghany, Aya; Awad, Alaa; Defrawy, Inas El; Shemis, Mohamed

doi:10.1186/s43042-019-0032-3

Cited by 11 publications

(9 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, an almost similar prevalence rate of HLGR was reported in a study by Tian et al (2019) in china [44] . Diab et al (2019) [45] showed that 78% of isolates were HLGR, which is higher in comparison to this study result.…”

Section: Resultscontrasting

confidence: 78%

Prevalence of Vancomycin and Gentamycin Resistance among Enterococci spp. in Iran during 2007-2019: A Systematic Review

Arfaatabar

Shahbazi²,

Ebrahimi³

2022

IEM

View full text Add to dashboard Cite

Backgrounds: Enterococci are Gram-positive bacteria that colonize the intestine of warmblooded animals and humans as normal flora. Enterococci cause a variety of communityacquired and nosocomial infections. The emergence of vancomycin and gentamicin resistant enterococci has made a major challenge in the treatment of enterococcal infections worldwide. Therefore, the present study was conducted to evaluate the prevalence of vancomycin and gentamycin resistance among Enterococcus spp in Iran during 2007-2019. Materials & Methods: In this study, 26 studies were reviewed to collect data on the frequency of vancomycin and gentamicin resistant enterococci in Iran. To find studies published during January 2007 to January 2019, a search strategy was performed by searching different Iranian and international databases, including SID, Google Scholar, Scopus, Medline, Pub Med, and Web of Science. Findings: The prevalence of vancomycin-and gentamicin-resistant enterococci was very high in Iran (41 and 44%, respectively). Accordingly, Enterococcus faecalis was more prevalent in clinical samples compared to E. faecium (75.49% vs. 24.05%). However, resistance to vancomycin was higher in E. faecium strains compared to E. faecalis. Conclusion: Due to the increasing vancomycin and gentamicin resistance among Enterococcus species in Iran, it is necessary to design strategies that lead to the rational prescription of antibiotics and limit the spread of resistant enterococci.

show abstract

Section: Resultscontrasting

confidence: 78%

Prevalence of Vancomycin and Gentamycin Resistance among Enterococci spp. in Iran during 2007-2019: A Systematic Review

Arfaatabar

Shahbazi²,

Ebrahimi³

2022

IEM

View full text Add to dashboard Cite

show abstract

“…The role of the APH(3’)-IIIa gene, as found in the DA70870 isolate, in providing resistance to aminoglycoside antibiotics such as kanamycin and streptomycin in S . aureus and Enterococci is well established [ 84 , 85 ]. Furthermore, among aminoglycoside-modifying enzymes, this particular type is less prevalent in S .…”

Section: Discussionmentioning

confidence: 99%

High prevalence of heteroresistance in Staphylococcus aureus is caused by a multitude of mutations in core genes

Heidarian,

Guliaev,

Nicoloff

et al. 2024

PLoS Biol

View full text Add to dashboard Cite

Heteroresistance (HR) is an enigmatic phenotype where, in a main population of susceptible cells, small subpopulations of resistant cells exist. This is a cause for concern, as this small subpopulation is difficult to detect by standard antibiotic susceptibility tests, and upon antibiotic exposure the resistant subpopulation may increase in frequency and potentially lead to treatment complications or failure. Here, we determined the prevalence and mechanisms of HR for 40 clinical Staphylococcus aureus isolates, against 6 clinically important antibiotics: daptomycin, gentamicin, linezolid, oxacillin, teicoplanin, and vancomycin. High frequencies of HR were observed for gentamicin (69.2%), oxacillin (27%), daptomycin (25.6%), and teicoplanin (15.4%) while none of the isolates showed HR toward linezolid or vancomycin. Point mutations in various chromosomal core genes, including those involved in membrane and peptidoglycan/teichoic acid biosynthesis and transport, tRNA charging, menaquinone and chorismite biosynthesis and cyclic-di-AMP biosynthesis, were the mechanisms responsible for generating the resistant subpopulations. This finding is in contrast to gram-negative bacteria, where increased copy number of bona fide resistance genes via tandem gene amplification is the most prevalent mechanism. This difference can be explained by the observation that S. aureus has a low content of resistance genes and absence of the repeat sequences that allow tandem gene amplification of these genes as compared to gram-negative species.

show abstract

“…A moderate level of resistance was observed among high-level streptomycin in the present study. Although there is bactericidal activity among enterococci towards aminoglycosides, those antibiotics can be used together with cell wall inhibitors, such as ampicillin, for a synergistic effect, achieving a better outcome in the treatment of enterococcal infection [ 27 ]. Moreover, such combinations were considered in the treatment of infective endocarditis caused by E. faecalis [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

Resistance towards Critically Important Antimicrobials among Enterococcus faecalis and E. faecium in Poultry Farm Environments in Selangor, Malaysia

et al. 2022

View full text Add to dashboard Cite

Multidrug resistant (MDR) enterococci pose significant public health challenges. However, the extent of resistance in the environment is less explored. This study aimed to determine the antibiotic resistance in a poultry farm environment. Eighty enterococcal isolates recovered from the soil and effluent water of 28 poultry farms in Selangor state were included in the study for further bacterial identification and antibiotic susceptibility testing using a VITEK 2 system. Data were analyzed using Statistical Package for Social Science (SPSS) version 27. The resistance rate and MDR of enterococcal isolates were reported. Out of 80 isolates recovered, 72 (90%) exhibited resistance to at least one antibiotic, with 50 isolates (62.5%) being found to be MDR. All linezolid-resistant enterococci (LRE) exhibit MDR, which constituted 40% of resistance among all the isolates recovered from poultry environment. Since linezolid is listed as critically important antibiotics for clinical use by the World Health Organization (WHO), the higher resistance towards it and other critically important antibiotic for human use is a serious concern. Hence, relevant agencies need to investigate the use of clinically important antimicrobials in poultry farms paying special attention towards linezolid or any other antibiotics that can facilitate the development of LRE.

show abstract

Detection of high level aminoglycoside resistance genes among clinical isolates of Enterococcus species

Cited by 11 publications

References 14 publications

Prevalence of Vancomycin and Gentamycin Resistance among Enterococci spp. in Iran during 2007-2019: A Systematic Review

Prevalence of Vancomycin and Gentamycin Resistance among Enterococci spp. in Iran during 2007-2019: A Systematic Review

High prevalence of heteroresistance in Staphylococcus aureus is caused by a multitude of mutations in core genes

Resistance towards Critically Important Antimicrobials among Enterococcus faecalis and E. faecium in Poultry Farm Environments in Selangor, Malaysia

Contact Info

Product

Resources

About