Detection of HGV RNA in Digestive Organs

Shimizu, Toshihiro; Moriyama, Mitsuhiko; Arakawa, Yasuyuki

doi:10.1159/000050025

Cited by 2 publications

(2 citation statements)

References 24 publications

(23 reference statements)

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“…Hijikata et al [24] reported that there are no differences in clinical background factors among genotypes 1a, 1b and others. We reported previously the frequency of genotype 1b TTV DNA in non-B, non-C and type C liver diseases at the Nihon University Hospital, which is located in Tokyo [19,20,25].…”

Section: Discussionmentioning

confidence: 99%

TT Virus Infection Does Not Affect the Clinical Profiles of Patients with Hepatitis B and C in Yanbian City, China

et al. 2003

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China is an area of high endemicity for viral hepatitis, and the molecular epidemiological investigation of TT virus (TTV) infection is of interest. In the present study, we investigated the epidemiology, clinical significance and molecular characteristics of TTV infection in patients with chronic hepatitis B and C in Yanbian City, China. Serum samples obtained from 74 patients with hepatitis B and hepatitis C who visited Yanbian Hospital, located in northeast China, were analyzed in this study. The study group included 22 cases of chronic hepatitis B (B-CH), 17 cases of liver cirrhosis B (B-LC), 7 cases of hepatocellular carcinoma (B-HCC), 16 cases of chronic hepatitis C (C-CH), 11 cases of liver cirrhosis C (C-LC) and 1 case of hepatocellular carcinoma (C-HCC). Detection of TTV DNA was performed as described by Nishizawa et al. The second-round PCR products from 7 subjects were sequenced, followed by investigation of nucleotide homology and phylogenetic analysis. TTV DNA was present in 18.2, 5.9, 28.6, 6.3, 9.1 and 0% of the patients with B-CH, B-LC, B-HCC, C-CH, C-LC and C-HCC, respectively. The highest prevalence of TTV infection was seen in the groups aged 40–50 and over 60 years. There was no significant correlation between the presence of TTV DNA and the clinical parameters in patients with hepatitis B and C. The various isolates showed 97.9–100% with isolates reported previously from Japan and 98.4–100% with isolates reported previously from China. Nucleotide sequence analysis revealed that the Yanbian isolates could be classified in the same group as the Japan and China isolates. We concluded that chronic coinfection with TTV did not affect the serological features of chronic hepatitis B and C in China, as found in Tokyo, Japan.

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Section: Discussionmentioning

confidence: 99%

TT Virus Infection Does Not Affect the Clinical Profiles of Patients with Hepatitis B and C in Yanbian City, China

et al. 2003

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“…In addition, the levels of immunoglobulin G in these patients were less than 2,000 mg/ml. Furthermore, hepatitis B virus (HBV) DNA, GB-virus C/hepatitis G virus (GBV-C/HGV) RNA, TT virus (TTV) DNA and SEN-virus (SEN-V) DNA were not detected by nested polymerase chain reaction methods [9][10][11][12]. No heavy drinkers (more than 30 g/day of ethanol intake) were included in the study.…”

Section: Patientsmentioning

confidence: 99%

Removal of Hepatitis C Virus by G-1 Beads in Sera from Patients with Chronic Hepatitis C

Moriyama

Kushima²,

Matsumura³

et al. 2005

Intervirology

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Recently, a new method of extracorporeal granulocyte depletion apheresis has been developed to treat inflammatory systemic diseases using an Adacolumn (Japan Immunoresearch Laboratories, Takasaki, Japan) that is filled with acetate cellulose beads (G-1 beads) to adsorb the granulocytes. We examined whether hepatitis C virus (HCV) is adsorbed after incubation of the Adacolumn with the sera from patients with HCV-RNA-positive chronic hepatitis C. Patients and Methods: A total of 10 patients with chronic hepatitis C, whose levels of HCV RNA were greater than 800 kIU/ml were examined. The serum was incubated with 500 G-1 beads in a syringe at 37°C for 1 h. After removal of the serum, the beads were washed with RNase-free water. The G-1 beads were removed from the syringe after centrifugation. RNA was extracted from 200 µl of the wash waste and from 10, 50, 100 and 200 beads, respectively, using TRIZol regent. Detection of HCV RNA was performed using the nested PCR method. Results: HCV RNA was detected from as few as 10 G-1 beads. HCV RNA was not detected from waste fluid collected after the last wash from any of the patients. Further, HCV RNA was detected in the initial waste fluid after the 37°C incubation with serum in all of the patients. Since HCV RNA was detected on the G-1 beads, but not from the last washing solution in the current examination, these results suggest that the G-1 beads adsorbed HCV RNA. Conclusions: Our in vitro study confirmed that G-1 beads adsorbed HCV; therefore, apheresis using a column filled with G-1 beads may reduce the HCV RNA load in the blood of patients with chronic hepatitis C.

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Detection of HGV RNA in Digestive Organs

Cited by 2 publications

References 24 publications

TT Virus Infection Does Not Affect the Clinical Profiles of Patients with Hepatitis B and C in Yanbian City, China

TT Virus Infection Does Not Affect the Clinical Profiles of Patients with Hepatitis B and C in Yanbian City, China

Removal of Hepatitis C Virus by G-1 Beads in Sera from Patients with Chronic Hepatitis C

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