Detection of herpes simplex virus type 1 in addition to Epstein–Bar virus in tonsils using a new multiplex polymerase chain reaction assay

Şahin, Fikret; Gerçeker, Devran; Karasartova, Djursun; Ozsan, T. Murat

doi:10.1016/j.diagmicrobio.2006.09.013

Cited by 22 publications

(16 citation statements)

References 23 publications

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“…HSV2 is principally transmitted by sexual intercourse and is less frequent with a seroprevalence 12% to 32% among immunocompetent adults . The present study found an HSV1 prevalence of around 3% in children and adults, findings which are relatively consistent with the little available literature, (6.3% and 8.6% in two small studies including children and adults) . HSV2 was detected only in two (0.4%) tonsil brushings from adults, and not in children.…”

Section: Discussionsupporting

confidence: 90%

“…HSV2 was detected only in two (0.4%) tonsil brushings from adults, and not in children. HSV2 is indeed probably rare in the oropharynx and was to date never detected in tonsils …”

Section: Discussionmentioning

confidence: 99%

“…Palatine tonsils, a secondary lymphoid organ triggering immune responses against inhaled or ingested microorganisms, may serve as a reservoir for several HHV species. Indeed, previous studies reported the detection of HSV1, EBV, CMV, HHV6, HHV7, and KSHV in tonsil specimens . Of particular interest, data on Waldeyer's ring infection with EBV are crucial, as a recognized cause of nasopharyngeal carcinoma and lymphomas.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence of human herpesviruses infections in nonmalignant tonsils: The SPLIT study

Kourieh

Gheit

Tommasino

et al. 2018

Journal of Medical Virology

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Objective To assess the prevalence of all known human herpesviruses (HHV) in tonsils of an age‐stratified large sample of immunocompetent children and adults. Methods Patients undergoing tonsillectomy for benign indications were recruited in 19 French hospitals. After resection, the entire outer surfaces of right and left half tonsils were extensively brushed. A highly sensitive species‐specific multiplex assay was used to detect herpes simplex virus 1 (HSV1), HSV2, Epstein‐Barr virus (EBV; types 1 and 2), and human cytomegalovirus (CMV) DNA in 688, as well as varicella zoster virus (VZV), HHV6A, HHV6B, HHV7, and Kaposi's sarcoma‐associated herpesvirus (KSHV) DNA in a subset of 440 tonsil brushings. Results Overall 85% of tonsil brushing samples were infected with at least one HHV species. HHV7 and EBV were the most prevalent (≈70%), followed by HHV6B (≈50%), HSV1, CMV, VZV (≈2%), and KSHV and HSV2 (<1%), while HHV6A was not detected. EBV prevalence was significantly higher in adults than in children, whereas it was opposite for HHV6B and VZV. No difference in HHV prevalence was observed by sex. In multivariate analysis, EBV detection was associated with age greater than or equal to 15 years (prevalence ratio [PR] = 1.8; 95% confidence interval [CI]: 1.5‐2.3) and marginally with tobacco smoking (PR = 1.2; 95% CI: 1.1‐1.3). Conclusion Differing patterns of HHV infection in tonsils in a large age‐stratified population were described. This study is by far the largest available and shows that EBV, HHV6B, and HHV7 are commonly detected in the tonsils in both men and women, in contrast to other HHVs.

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Section: Discussionsupporting

confidence: 90%

“…HSV2 was detected only in two (0.4%) tonsil brushings from adults, and not in children. HSV2 is indeed probably rare in the oropharynx and was to date never detected in tonsils …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prevalence of human herpesviruses infections in nonmalignant tonsils: The SPLIT study

Kourieh

Gheit

Tommasino

et al. 2018

Journal of Medical Virology

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“…Como control positivo se usaron células mononucleares de sangre periférica de un paciente con infección confirmada por CMV (22). Para la detección del EBV se empleó el protocolo reportado por Sahin, et al, con el cual se amplifica un producto de 176 pb (23). El control positivo consistió en ADN de células TK6, las cuales, como se sabe, son positivas para este virus.…”

Section: Extracción De Adnunclassified

Descarga de HSV-1, HSV-2, CMV y EBV en la saliva de receptores de trasplante de precursores hematopoyéticos de la Fundación HOMI - Hospital de la Misericordia, Bogotá, D. C.

et al. 2016

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Introducción. El trasplante de precursores hematopoyéticos es una alternativa en el tratamiento de diversas condiciones en la población pediátrica. La intensidad del acondicionamiento para el trasplante predispone al desarrollo de complicaciones en los receptores. Las infecciones por el virus herpes simple 1 (HSV-1), el virus herpes simple 2 (HSV-2), el citomegalovirus (CMV) humano y el virus de Epstein-Barr (EBV) son una causa importante de morbimortalidad en estos pacientes. La reactivación de infecciones latentes puede producir descargas virales asintomáticas detectables en la saliva, lo cual ayuda a determinar el comportamiento de dichas infecciones en pacientes con trasplante y a establecer el diagnóstico temprano de la reactivación. Objetivo. Evaluar el comportamiento de la descarga viral de HSV-1, HSV-2, CMV y EBV en la saliva de pacientes hospitalizados en la Unidad de Trasplante de la Fundación HOMI - Hospital de la Misericordia, entre enero y noviembre de 2012. Materiales y métodos. Se evaluaron muestras de saliva de 17 receptores de trasplante. La presencia de ADN de HSV-1, HSV-2, CMV y EBV en las muestras de saliva se detectó mediante reacción en cadena de la polimerasa convencional. Resultados. Se detectó el ADN del HSV-2 en la saliva de cuatro pacientes, del CMV en la de cuatro y del EBV en la de nueve, lo cual se asoció con leucopenia. Cuatro de los 17 pacientes presentaron cargas simultáneas de CMV y EBV. No se detectó el ADN del HSV-1. Conclusiones: Se demostró una descarga asintomática de HSV-2, CMV y EBV asociada a leucopenia en la saliva de los pacientes.

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“…Viral culture and identification of HSV has been accepted as the gold standard for the laboratory diagnosis of HSV infection, but in a recent study by Espy et al [16] use of PCR in the routine clinical laboratory provided a system that achieved a 22 % increase in sensitivity compared with shell vial cell culture, and was obtained in a shorter time [11]. Multiplex PCR assay has been used to show tonsils may be a reservoir for HSV1, playing a role in recurrent tonsillitis and the development of systemic disease [17]. Establishment of the airway should be the corner stone of any management protocol followed by targeted treatment of the underlying cause of the infection.…”

Section: Discussionmentioning

confidence: 99%

Acute Necrotizing Herpetic Tonsillitis: A Report of Two Cases

Borhan

Dababo

Thompson

et al. 2013

Head and Neck Pathol

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The finding of herpetic tonsillitis is rare. Tonsillectomies are usually done for children with recurrent chronic tonsillitis, while viral throat infections are generally self-limiting. We present two cases: A 5 yearold girl, with atypical hemolytic anemia managed with Eculizumab, who presented with a pharyngeal infection and tonsillar enlargement that did not respond to intravenous antibiotics or antifungal therapies; and a 30 yearold man who presented with upper airway obstruction and fever; bilateral tonsillectomies were performed. Histopathological examination showed a necrotizing tonsillitis with numerous ground-glass intranuclear inclusions, characteristic of herpes viral infection, further confirmed by Herpes simplex virus in situ hybridization. Both patients were managed by intravenous Acyclovir, with dramatic improvement.

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Detection of herpes simplex virus type 1 in addition to Epstein–Bar virus in tonsils using a new multiplex polymerase chain reaction assay

Cited by 22 publications

References 23 publications

Prevalence of human herpesviruses infections in nonmalignant tonsils: The SPLIT study

Prevalence of human herpesviruses infections in nonmalignant tonsils: The SPLIT study

Descarga de HSV-1, HSV-2, CMV y EBV en la saliva de receptores de trasplante de precursores hematopoyéticos de la Fundación HOMI - Hospital de la Misericordia, Bogotá, D. C.

Acute Necrotizing Herpetic Tonsillitis: A Report of Two Cases

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