Detection of Herpes Simplex Virus in Clinical Specimens by an Enzyme-linked Immunosorbent Assay

Lawrence, Timothy G.; Budzko, Delia B.; Wilcke, Burton W.

doi:10.1093/ajcp/81.3.339

Cited by 15 publications

(15 citation statements)

References 3 publications

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“…The overall rate of HSV detection in our monoclonal and polyclonal assays was disappointingly low but comparable to that reported by other investigators using different antibodies and assay modifications [Adler Storthz et al, 1983;Lawrence et al, 1984;Warford et al, 19841. Other groups, using the same polyclonal antibodies we used, have reported a detection rate approaching [Grillner and Landqvist, 19831 or equivalent to virus isolation [Vestergaard and Jensen, 19811.…”

Section: Discussionsupporting

confidence: 87%

Factors influencing the sensitivity of herpes simplex virus detection in clinical specimens in a simultaneous enzyme‐ linked immunosorbent assay using monoclonal antibodies

Clayton

Beckford

Roberts

et al. 1985

Journal of Medical Virology

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A rapid simultaneous enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies was investigated for herpes simplex virus (HSV) detection. All HSV isolated (n = 127) were detected, whereas no response was obtained with HSV negative preparations. Equivalent results were obtained from 275 of 277 clinical specimens in the monoclonal ELISA and in an ELISA using polyclonal antibodies, confirming that appropriately selected monoclonal antibodies may be as efficacious as polyclonal antibodies in antibody-based assays. In clinical specimens, the rate of HSV detection (sensitivity) relative to tissue culture isolation was low for both assays, and the major factor responsible for this was the low concentration of virus present in some specimens. The sensitivity of ELISA obtained in routine use varied with different panels of unselected specimens and was related to the speed of development of the cytopathic effect. These results emphasise the need for caution in assigning a definitive sensitivity level to ELISA tests evaluated on different panels of specimens.

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Section: Discussionsupporting

confidence: 87%

Factors influencing the sensitivity of herpes simplex virus detection in clinical specimens in a simultaneous enzyme‐ linked immunosorbent assay using monoclonal antibodies

Clayton

Beckford

Roberts

et al. 1985

Journal of Medical Virology

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“…The use of selected specimens may partly explain the wide variation in antigen detection rate achieved by different groups [Vestergaard and Jensen, 1981 ;Adler Storthz et al, 1983;Grillner and Landqvist, 1983;Lawrence et al, 1984;Morgan and Smith, 1984;Nerurkar et al, 1984;Warford et al, 1984;Alexander et al, 1985;Clayton et al, 19851. For this reason it is prudent to use the same panel of unselected specimens for comparative evaluation studies.…”

Section: Discussionmentioning

confidence: 99%

Herpes Simplex Virus Detection by ELISA: Effect of Enzyme Amplification, Nature of Lesion Sampled and Specimen Treatment

Clayton

Roberts

Chantler

et al. 1986

Journal of Medical Virology

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The relative sensitivity of two enzyme detection procedures was investigated in a simultaneous "monoclonal" ELISA for herpes simplex virus (HSV). A cyclical enzyme amplified detection system with alkaline phosphatase, rather than horse-radish peroxidase and a conventional chromogenic substrate, gave an increase in absolute sensitivity and a 20 to 30% increase in the detection of HSV in routine isolation-positive genital specimens collected in transport medium. The HSV detection rate, with both procedures, was shown to vary with the site and clinical stage of lesion sampled; it was highest with penile vesicular lesions. Direct extraction of the swab specimen in a small volume of diluent further increased the sensitivity of antigen detection giving positive and negative predictive values of 100 and 96% respectively. The overall sensitivity of HSV detection was equivalent to that obtained by isolation in cell culture. The amplified ELISA offers an alternative, rapid, simple, non-culture technique for routine HSV diagnosis that does not rely upon retention of virus viability.

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“…The unusual circumstances made use of a single reagent feasible (27). Because (80,83,104,108). Sensitivity of these methods varies from 50 to 96% and specificity varies from 91 to 100%.…”

Section: Serologymentioning

confidence: 99%

“…When a patient is considered to have HSV CNS disease, specimens are collected from biopsied brain tissue or CSF or both. Direct IF or ELISA staining of brain tissue, using specific HSV antibody, can be performed, and results are available within a few hours (80,83,104,108,128). Culture results for confirmation are generally available in 1 to 5 days (94).…”

Section: Influence Of the Virology Laboratory Onmentioning

confidence: 99%

Role of the virology laboratory in diagnosis and management of patients with central nervous system disease

1989

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A number of viruses cause acute central nervous system disease. The two major clinical presentations are aseptic meningitis and the less common meningoencephalitis. Clinical virology laboratories are now more widely available than a decade ago; they can be operated on a modest scale and can be tailored to the needs of the patients they serve. Most laboratories can provide diagnostic information on diseases caused by enteroviruses, herpesviruses, and human immunodeficiency virus. Antiviral therapy for herpes simplex virus is now available. By providing a rapid diagnostic test or isolation of the virus or both, the virology laboratory plays a direct role in guiding antiviral therapy for patients with herpes simplex encephalitis. Although there is no specific drug available for enteroviruses, attention needs to be paid to these viruses since they are the most common cause of nonbacterial meningitis and the most common pathogens causing hospitalization for suspected sepsis in young infants in the United States during the warm months of the year. When the virology laboratory maximizes the speed of viral detection or isolation, it can make a significant impact on management of these patients. Early viral diagnosis benefits patients with enteroviral meningitis, most of whom are hospitalized and treated for bacterial sepsis or meningitis or both; these patients have the advantage of early withdrawal of antibiotics and intravenous therapy, early hospital discharge, and avoidance of the risks and costs of unnecessary tests and treatment. Enteroviral infection in young infants also is a risk factor for possible long-term sequelae. For compromised patients, the diagnostic information helps in selecting specific immunoglobulin therapy. Good communication between the physician and the laboratory will result in the most benefit to patients with central nervous system viral infection.

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Detection of Herpes Simplex Virus in Clinical Specimens by an Enzyme-linked Immunosorbent Assay

Cited by 15 publications

References 3 publications

Factors influencing the sensitivity of herpes simplex virus detection in clinical specimens in a simultaneous enzyme‐ linked immunosorbent assay using monoclonal antibodies

Factors influencing the sensitivity of herpes simplex virus detection in clinical specimens in a simultaneous enzyme‐ linked immunosorbent assay using monoclonal antibodies

Herpes Simplex Virus Detection by ELISA: Effect of Enzyme Amplification, Nature of Lesion Sampled and Specimen Treatment

Role of the virology laboratory in diagnosis and management of patients with central nervous system disease

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