Detection of herpes simplex and varicella-zoster virus from skin lesions: comparison of RT-PCR and isothermal amplification for rapid identification

Jevšnik, Monika; Lusa, Lara; Uršič, Tina; Biškup, Urška Glinšek; Petrovec, Miroslav

doi:10.1016/j.diagmicrobio.2020.115015

Cited by 12 publications

(4 citation statements)

References 18 publications

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“…However, all subjects with available medical records were examined by a rapid examination kit. Moreover, concordance between the rapid identification kit and RT-PCR was 99.3% [ 28 ]. It is, therefore, considered that the inconsistency of diagnosis between medical institutions would be negligible.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Live Attenuated Varicella-Zoster Vaccine in Adults Older than 50 Years in Japan: A Retrospective Cohort Study

et al. 2023

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Background: In Japan, freeze-dried live attenuated varicella-zoster vaccine BIKEN is available for adults aged ≥50 years to prevent herpes zoster (HZ). A prospective cohort study of 1200 healthy adults and 300 patients with underlying illness confirmed vaccine safety between 2016 and 2017. However, evidence of vaccine effectiveness (VE) is limited. Methods: VE against HZ and postherpetic neuralgia (PHN) was evaluated in the vaccinated cohort of the previous safety study in a follow-up study between 2021 and 2022 and compared with unvaccinated family members. Self-administered questionnaires determined retrospective experiences of HZ and PHN diagnosis. Logistic regression estimated the VE by calculating the outcome odds ratio (OR) in vaccinated vs. unvaccinated groups: VE = (1 − OR) × 100(%). Results: Overall, 1098 vaccinated and 518 unvaccinated subjects were analysed. Between 2016 and 2022, 26 vaccinated (2.4%) and 22 unvaccinated (4.2%) subjects reported HZ diagnosis, and 3 vaccinated (0.3%) and 2 unvaccinated (0.4%) subjects reported PHN. Adjusted VE against a clinical diagnosis was 41% for HZ [−6% to 67%], with marginal significance, and 16% [−408% to 86%] for PHN. Stratification by age, sex, or comorbidities had an adjusted VE against HZ of ~40%, which was similar between strata. Conclusion: Freeze-dried live attenuated varicella-zoster vaccine reduces the risk of HZ regardless of age, sex, or comorbidities.

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Section: Discussionmentioning

confidence: 99%

Effectiveness of Live Attenuated Varicella-Zoster Vaccine in Adults Older than 50 Years in Japan: A Retrospective Cohort Study

et al. 2023

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“…As with other isothermal reactions, only simple heating apparatus is required, however, the higher temperature reaction of HDA negates the ability to use ambient temperatures for amplification. HDA functions in the presence of other biological agents, and can be used alongside crude samples, without the need for DNA or RNA extraction (Kim et al, 2011;Li et al, 2013;Jevšnik et al, 2020).…”

Section: Helicase-dependent Amplificationmentioning

confidence: 99%

Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods

Cassedy

Parle‐McDermott

O’Kennedy

2021

Front. Mol. Biosci.

116

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Viruses are ubiquitous in the environment. While many impart no deleterious effects on their hosts, several are major pathogens. This risk of pathogenicity, alongside the fact that many viruses can rapidly mutate highlights the need for suitable, rapid diagnostic measures. This review provides a critical analysis of widely used methods and examines their advantages and limitations. Currently, nucleic-acid detection and immunoassay methods are among the most popular means for quickly identifying viral infection directly from source. Nucleic acid-based detection generally offers high sensitivity, but can be time-consuming, costly, and require trained staff. The use of isothermal-based amplification systems for detection could aid in the reduction of results turnaround and equipment-associated costs, making them appealing for point-of-use applications, or when high volume/fast turnaround testing is required. Alternatively, immunoassays offer robustness and reduced costs. Furthermore, some immunoassay formats, such as those using lateral-flow technology, can generate results very rapidly. However, immunoassays typically cannot achieve comparable sensitivity to nucleic acid-based detection methods. Alongside these methods, the application of next-generation sequencing can provide highly specific results. In addition, the ability to sequence large numbers of viral genomes would provide researchers with enhanced information and assist in tracing infections.

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“…Recent studies by Jevšnik et al have compared the effective detection of herpes simplex and varicella-zoster virus from skin lesions using a commercially available test, the RT-PCR (Argene, BioMerieux, Verniolle, France) test, performed on LC480 platform (Roche Applied Science, Mannheim, Germany) and the HDA assay (Solana HSV1 + 2/VZV assay, Quidel Corporation, San Diego, CA, USA). Both tests showed good agreement of 98.3% for HSV-1 and 99.3% for VZV [ 127 ]. The limit of detection of RT-PCR reagents was about 107 copies/mL.…”

Section: Detection Of Hsv In Lesionsmentioning

confidence: 99%

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

Nath

Kabir

Doust

et al. 2021

Infectious Disease Reports

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Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. HSV is highly contagious and can be transmitted via any type of physical contact. Additionally, viral shedding can also happen from asymptomatic infections. Thus, early and accurate detection of HSV is needed to prevent the transmission of this infection. Herpes can be diagnosed in two ways, by either detecting the presence of the virus in lesions or the antibodies in the blood. Different detection techniques are available based on both laboratory and point of care (POC) devices. Laboratory techniques include different biochemical assays, microscopy, and nucleic acid amplification. In contrast, POC techniques include microfluidics-based tests that enable on-spot testing. Here, we aim to review the different diagnostic techniques, both laboratory-based and POC, their limits of detection, sensitivity, and specificity, as well as their advantages and disadvantages.

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Detection of herpes simplex and varicella-zoster virus from skin lesions: comparison of RT-PCR and isothermal amplification for rapid identification

Cited by 12 publications

References 18 publications

Effectiveness of Live Attenuated Varicella-Zoster Vaccine in Adults Older than 50 Years in Japan: A Retrospective Cohort Study

Effectiveness of Live Attenuated Varicella-Zoster Vaccine in Adults Older than 50 Years in Japan: A Retrospective Cohort Study

Virus Detection: A Review of the Current and Emerging Molecular and Immunological Methods

Diagnosis of Herpes Simplex Virus: Laboratory and Point-of-Care Techniques

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