Detection of hepatitis G virus RNA in patients with hepatitis B, hepatitis C, and non-A-E hepatitis by RT-PCR using multiple primer sets

Xh, Zhang; Shinzawa, H.; Shao, Li; Ishibashi, Masamichi; Saito, Koji; Ohno, Shinji; Yamada, Nobuo; Misawa, Hiroyuki; Togashi, Hitoshi; Takahashi, Tsuneo

doi:10.1002/(sici)1096-9071(199708)52:4<385::aid-jmv7>3.0.co;2-y

Cited by 17 publications

(4 citation statements)

References 27 publications

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“…There are a few reports of the influence of primer combination on the detection of HGV-RNA. The 5 0 NC region primers appear to be the most effective in detecting the HGV-RNA as compared to those derived from the NS3 region [30]. We used the NS3-region-derived primers, as the protocol was already standardized in our laboratory for the detection of HGV-RNA.…”

Section: Discussionmentioning

confidence: 91%

Hepatitis-G Virus Infection in Multi-transfused Patients and Intravenous Drug Abusers: New Delhi Experience

et al. 2007

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Hepatitis G virus (HGV) is a newly described virus that has been implicated in transfusion-associated hepatitis. HGV-RNA indicating current infection has frequently been isolated from the sera of multi-transfused patients (MTPs) and that of intravenous drug abusers (IVDAs). There is insufficient data on its prevalence among these high-risk groups in India. Hence this study was designed to evaluate the prevalence of HGV infection in MTPs and IVDAs. A total of 90 (57 males and 33 females) MTPs and 65 (44 males and 21 females) IVDAs were recruited in the study. Patients' sera were tested for liver function profile, HGV-RNA, anti-HCV, HCV-RNA, HBsAg, and HBV-DNA. HGV-RNA was detected in 17.7% (16/90) MTPs and 23% (15/65) IVDAs, and 93.5% of our HGV-positive patients were found to be co-infected with either HBV (38.7%) or HCV (74.1%). The sero-prevalence of HBV was found out to be 40% and 21.5%, respectively, in the two groups, that of HCV was 32.2% and 32.3%, respectively, and dual infection was observed in 15.5% and 10.7% of the subjects, respectively. There were no statistically significant differences between the clinical, biochemical, and serological profiles of HGV-positive and negative patients. The prevalence of HGV infection in these high-risk groups is significantly higher than in controls. HGV is found to be co-infected more frequently with HCV than with HBV.

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Section: Discussionmentioning

confidence: 91%

Hepatitis-G Virus Infection in Multi-transfused Patients and Intravenous Drug Abusers: New Delhi Experience

et al. 2007

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“…One remarkable finding in our study was significant variation between the nucleotide sequences of NS3 of HGV although the sequences seemed much more conserved than corresponding HCV sequences ( 14 ). The lower than expected variability of HGV may have been indicative of weak selection pressure on these viruses, due to an inadequate immune response.…”

Section: Discussionmentioning

confidence: 59%

“…Attempts to group phylogenetic of GBV-C/HGV isolates based on sequence analysis of subgenomic coding regions need further studies. For instance, comparison of sequences within the NS3 helicase region for isolates failed to differentiate between African, Asian, and European-North American isolates ( 14 , 34 ).…”

Section: Discussionmentioning

confidence: 99%

“…While the NS3 or helicase region is not considered to be ideal for genotyping of the HGV, 5’ UTR and E2 information help to better identifying genotype and characteristics of HGV isolates ( 13 , 14 ). Analyzing of NS3 region of HGV was reported in some countries such as Spain, Germany, China, the United States, and the United Kingdom ( 15 - 18 ).…”

Section: Introductionmentioning

confidence: 99%

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Sequencing and Phylogenetic Study of Partial NS3 Gene of Iranian GB Virus C/Hepatitis G Virus (HGV) Originated From Hemodialysis Patients in Tehran

et al. 2015

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Background:Hepatitis viruses are one of the most important concerns in the patients on hemodialysis who are at high risk for blood-borne infections. GB virus C (GBV-C)/hepatitis G virus (HGV) is positive-stranded RNA virus with global distribution, which codes structural and nonstructural proteins (such as NS3).Objectives:Review on literature about Iranian HGV isolates showed that there was no report on sequencing and phylogenetic characteristics of NS3 gene and therefore, this study attempted to answers these challenges.Materials and Methods:Briefly, steps followed were as follows: preparation of nucleic acids from three Iranian hemodialysis HGV-positive samples and RNA extraction; reverse transcriptase-PCR (RT-PCR) for amplification of NS3 gene and gel electrophoresis; sequencing and analyzing sequencing data; NCBI Registering; blasting and alignment of sequences; and finally constructing the phylogenetic tree.Results:Nucleotide BLAST results emphasized that most similar sequences to Ir NS3 sequences were those from Europe (Spain, Germany, and the United Kingdom) and the United states and IR sequences were located with five leaves in a branch. In addition, homology of Ir sequences showed96.4% (between IR1 and IR2), 94.9% identity (between IR1 and IR3), and 96.5% identity (between IR2 and IR3).Furthermore, highly variation and significance differences in NS3 between GBC isolates from geographical regions and some little changes in IR sequences were seen. Finally, phylogenetic tree revealed that NS3 genotype of Iranian isolates was probably similar to European countries and USA.Conclusions:Overall, results of present study were consistent with the data reported earlier base on 5’ NTR in Iranian isolates and revealed genotype IR2 is major genotype in Iranian HGV-positive patient. It means there are higher similarities between Iranian and Europe-USA in HGV NS3 gene.

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Epidemiological study and genetic analysis of GB virus C infection in general population from an area endemic for hepatitis C

et al. 1998

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The aim of this work was to study the prevalence, potential risk factors, clinical and laboratory features of GB virus C (GBV-C) infection in general population from an area endemic for hepatitis C. A reverse transcriptase-polymerase chain reaction (RT-PCR) for detection of GBV-C RNA was used to examine the prevalence of GBV-C RNA in both hepatitis C virus (HCV) endemic (R town) and nonendemic areas (M town) in Yamagata prefecture, Japan. In R town, GBV-C RNA was detected in 23 (2.9%) out of the 800 residents, whereas anti-HCV and HCV-RNA were found in 226 (28.3%) and 163 (20.4%), respectively. The prevalence of GBV-C RNA in R town (2.9%) was higher than that in M town (1.0%), although the difference was not statistically significant. The individuals with anti-HCV had significantly higher frequency of active GBV-C-infection than those without anti-HCV in both towns. No evidence indicating that GBV-C infection affected the severity of hepatitis C was obtained. The multivariate analysis revealed that the young anti-HCV positive individuals with a history of blood transfusion had higher incidence of active GBV-C infection. The phylogenetic analysis showed that the GBV-C isolates from both R and M towns were divided into two separate branch groups designated HG and Asia GB groups.

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Detection of hepatitis G virus RNA in patients with hepatitis B, hepatitis C, and non-A-E hepatitis by RT-PCR using multiple primer sets

Cited by 17 publications

References 27 publications

Hepatitis-G Virus Infection in Multi-transfused Patients and Intravenous Drug Abusers: New Delhi Experience

Hepatitis-G Virus Infection in Multi-transfused Patients and Intravenous Drug Abusers: New Delhi Experience

Sequencing and Phylogenetic Study of Partial NS3 Gene of Iranian GB Virus C/Hepatitis G Virus (HGV) Originated From Hemodialysis Patients in Tehran

Epidemiological study and genetic analysis of GB virus C infection in general population from an area endemic for hepatitis C

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