Detection of hepatitis E virus genotypes 3 and 4 in pig farms in Korea

Kim, Yong Hyun; Park, Byung Joo; Ahn, Hee-Seop; Han, Sang-Hoon; Go, Hyeon‐Jeong; Kim, Dong‐Hwi; Lee, Joong‐Bok; Park, Seung‐Yong; Song, Chang‐Seon; Choi, In‐Soo

doi:10.4142/jvs.2018.19.2.309

Cited by 16 publications

(12 citation statements)

References 15 publications

(27 reference statements)

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“…KY496215) and swine HEV-3 (GenBank No. MF095679) used in this study were obtained from the stool sample of a rabbit and a pig, respectively, as described in our previous studies [25,26]. The viral titers of both rabbit HEV and swine HEV-3, used to challenge rabbits, were adjusted to 10 6 genomic equivalent (GE) copy number/mL in 4% bovine albumin solution.…”

Section: Experimental Animals and Virusesmentioning

confidence: 99%

Cross-Species Transmission of Swine Hepatitis E Virus Genotype 3 to Rabbits

Han

Park

Ahn

et al. 2020

Viruses

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Hepatitis E virus (HEV) is a quasi-enveloped, positive-sense single stranded RNA virus. HEV continually expands the host ranges across animal species. In this study, the possibility of cross-species infection with swine HEV-3 was investigated using rabbits. A total of fourteen 8-week old, specific pathogen-free rabbits were divided into three experimental groups. Four rabbits were used as negative controls, four rabbits were infected with rabbit HEV as positive controls, and six rabbits were inoculated with swine HEV-3. HEV RNA were detected from serum and fecal samples after viral challenge. The levels of anti-HEV antibodies, pro-inflammatory cytokines (IL-1, IL-6, TNF-α and IFN-α), and liver enzymes (alanine and aspartate aminotransferases) were determined in serum samples. Histopathological lesions were examined in liver tissues. Viral RNA and anti-HEV antibodies were identified in rabbits inoculated with swine HEV-3 demonstrating positive infectivity of the virus. However, pro-inflammatory cytokine and liver enzyme levels in serum were not significantly elevated, and only mild inflammatory lesions were detected in the liver tissues of rabbits infected with swine HEV-3. These results suggest that swine HEV-3 can engage in cross-species transmission to rabbits, but causes only mild inflammation of the liver.

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Section: Experimental Animals and Virusesmentioning

confidence: 99%

Cross-Species Transmission of Swine Hepatitis E Virus Genotype 3 to Rabbits

Han

Park

Ahn

et al. 2020

Viruses

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“…In this study, we found both HEV-3 and HEV-4 in porcine livers sold at grocery markets in Korea. In a previous study, we detected both HEV-3 and HEV-4 in the fecal samples of growing pigs [14]. These results indicate that many pig populations reared in Korea may be infected with zoonotic HEV-3 and HEV-4.…”

mentioning

confidence: 55%

“…Recently, zoonotic infection by HEV-7 was reported in a patient who had consumed camel milk [9]. HEV-3 and HEV-4 have been identified in the fecal and liver samples of pigs in several countries such as Korea [10][11][12][13][14]. Particularly, HEV-3 and HEV-4 were detected in the serum samples of blood donors and patients with acute cryptogenic hepatitis in Korea [15,16].…”

mentioning

confidence: 99%

Identification of Hepatitis E Virus in Bovine and Porcine Raw Livers

Park

Ahn

et al. 2019

Journal of Microbiology and Biotechnology

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“…Hepatitis E virus is a zoonotic pathogen with pigs and wild boars serving as natural reservoirs, especially for zoonotic genotypes HEV‐3 and HEV‐4 . Considering the identification of HEV new isolations in disparate animal species, it is obvious that HEV has a broad host range including pigs, rabbits, cats, dogs, horses, rats, cows, sheep, goats and ferrets .…”

Section: Discussionmentioning

confidence: 99%

Detection of hepatitis E virus genotypes 3 and 4 in donkeys in northern China

Pan

Zhao²,

Shen

et al. 2019

Equine Veterinary Journal

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Summary Background Hepatitis E virus (HEV) is the causative agent of acute self‐limiting hepatitis in humans in developing countries. Hepatitis E virus RNA was first detected in donkeys in Spain, but little is known about the possible presence of HEV in donkeys in China. Objectives To investigate the prevalence of HEV in donkeys in northern China. Study design Investigation of the prevalence of HEV in donkeys using serological, molecular and phylogenetic approaches. Methods A total of 401 donkey serum specimens were tested for serological and molecular detection of HEV via enzyme‐linked immunosorbent assay and quantitative reverse transcription polymerase chain reaction. The amplified products were cloned in pMD18‐T vector and sequenced. The alignment and phylogenetic analysis of partial HEV ORF2 genes were compared with the corresponding sequences of the obtained HEV representative strains. Results Serological results showed that 49 donkeys (12.22%, 95% CI: 9.18–15.83%) were positive for anti‐HEV‐specific antibodies, and 17 donkeys (4.24%, 95% CI: 2.49–6.70%) were positive for HEV viral RNA. On the basis of sequence alignment and phylogenetic analysis, all isolated HEV strains belonged to genotype 3 (HEV‐3) or HEV‐4, sharing more than 76.2–96.3% identities with 67 other HEV representative strains of HEV‐1 to HEV‐8. Main limitations Further studies about the prevalence of HEV in organs or faecal samples from donkeys are needed to evaluate the possible role of HEV reservoir and to determine the risk factors associated with the transmission of this zoonotic virus in donkeys in China. Conclusions This is the first report documenting the molecular analysis of donkey HEV strains worldwide and the serological evidence of HEV infection in donkeys in northern China. The results suggest that young donkeys are more susceptible to HEV infection compared with older donkeys. Further investigation is required to determine whether donkeys should be considered reservoirs for zoonotic HEV. The Summary is available in Chinese ‐ see Supporting information

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Detection of hepatitis E virus genotypes 3 and 4 in pig farms in Korea

Cited by 16 publications

References 15 publications

Cross-Species Transmission of Swine Hepatitis E Virus Genotype 3 to Rabbits

Cross-Species Transmission of Swine Hepatitis E Virus Genotype 3 to Rabbits

Identification of Hepatitis E Virus in Bovine and Porcine Raw Livers

Detection of hepatitis E virus genotypes 3 and 4 in donkeys in northern China

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