Detection of Hepatitis C Virus RNA in blood and saliva of transfusion-dependent thalassemia patients diagnosed with Hepatitis C

Background DNA from many pathogens can be detected in saliva. However, the presence and quantity of Treponema pallidum DNA in syphilis patients in saliva is unknown. Methods A total of 234 syphilis patients with different stages and 30 volunteers were enrolled. Paired saliva and plasma samples were collected from all the participants. Consecutive saliva samples from 9 patients were collected every 4 hours following treatment. Treponema pallidum DNA in samples was determined by nested PCR and droplet digital PCR targeting polA and Tpp47. Results Treponema pallidum DNA detection rates in saliva and plasma were 31.0% (9/29) and 51.7% (15/29) in primary syphilis(p=0.11), 87.5% (63/72) and 61.1% (44/72) in secondary syphilis(p<0.001), 25.6%(21/82) and 8.5%(7/82) in latent syphilis (p=0.004), 21.6%(11/51) and 5.9%(3/51) in symptomatic neurosyphilis (p=0.021), respectively. The loads of Tpp47 and polA in saliva were median 627 copies/ml (range, 0-101200 copies/ml) and median 726 copies/ml (range, 0-117260 copies/ml) for syphilis patients, respectively. In plasma, however, the loads of Tpp47 and polA were very low: median 0 copies/ml (range, 0-149.6 copies/ml) and median 0 copies/ml (range, 0-176 copies/ml), respectively. The loads of Treponema pallidum DNA in saliva during treatment were fluctuating downward, and the clearance time was positively correlated with the loads of Treponema pallidum DNA before treatment. Conclusions The collection of saliva is noninvasive and convenient. The high loads of Treponema pallidum DNA in saliva and the reduction after treatment indicated that saliva can be not only a diagnostic fluid for syphilis, but also an indicator of therapeutic effectiveness.

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Effectiveness of Two Desensitizer Materials, Potassium Nitrate and Fluoride Varnish in Relieving Hypersensitivity After Crown Preparation

Abdollahi¹,

Jalalian²

2019

The Journal of Contemporary Dental Practice

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Aim: The aim of this clinical investigation was to compare the efficacy of fluoride varnish and potassium nitrate on the reduction of hypersensitivity of prepared teeth both pre and post cementation. Materials and methods: In this randomized clinical trial, patients who needed at least three single crowns fixed partial prosthesis were included. Two desensitizing agents including fluoride varnish and potassium nitrate were used in this study and compared to the control group (sterile saline). In each patient, each prepared tooth was randomly assigned to one group. The desensitizing agent was applied on prepared teeth immediately after tooth preparation. The sensitivity of the teeth was measured by means of an air sensitivity test based on the visual analog scale at five different time points: baseline (2 hours after preparation), 7 days after preparation, before cementation, after cementation and one-week post cementation. Data were analyzed by Kruskal-Wallis and Friedman tests with a significant level of 0.05. Results: Overall 300 vital abutment teeth needed full crown coverage in 100 patients were included. Both desensitizers' agents decreased the sensitivity of vital abutment teeth compared to the control group at every time intervals except at baseline (p = 0.089). There was no significant difference between fluoride varnish and potassium nitrate in the reduction of pre-cementation sensitivity while one week after cementation, sensitivity was more relieved by potassium nitrate compared to fluoride varnish (p = 0.023). Conclusion: The application of both desensitizers is effective in sensitivity reduction of vital abutment teeth. Potassium nitrate, however, is more eligible one week after cementation compared to fluoride varnish. Clinical significance: Both potassium nitrate and fluoride varnish are capable of desensitizing prepared tooth for the fixed partial denture.

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Detection of Hepatitis C Virus RNA in blood and saliva of transfusion-dependent thalassemia patients diagnosed with Hepatitis C

Cited by 3 publications

References 31 publications

Optimization of a real time PCR methodology for HCV RNA quantification in saliva samples

Optimization of a real time PCR methodology for HCV RNA quantification in saliva samples

A New Specimen for Syphilis Diagnosis: Evidence by High Loads ofTreponema pallidumDNA in Saliva

Effectiveness of Two Desensitizer Materials, Potassium Nitrate and Fluoride Varnish in Relieving Hypersensitivity After Crown Preparation

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