Detection of Hepatitis B Virus Pres1 Antigen Using a Time-Resolved Fluoroimmunoassay

Hu, Zhigang; Li, Mei; Huang, Biao; Liu, Jie; Lei, Yu; Guo-qian, Chen

doi:10.1080/15321819.2011.609576

Cited by 14 publications

(9 citation statements)

References 14 publications

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“…The superiorities of this method include its log-fold dynamic range, low cost, flexibility, and that it does not require the use of hazardous materials such as radioactive iodine and cyanogen bromide (13). More attention has been given to the TR-IFMA that has been proved to be the most promising approach for the quantitation and detection of many diagnostic trace protein markers (14)(15)(16)(17). The aim of this study was to establish a method of TR-IFMA with higher sensitivity and broader detection range for detecting serum L protein, and study the superiority of TR-IFMA over enzymelinked immunosorbent assay (ELISA).…”

Section: Introductionmentioning

confidence: 99%

Detection of Hepatitis B Virus Large Surface Protein Using a Time‐Resolved Immunofluorometric Assay

Liu

et al. 2014

Clinical Laboratory Analysis

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Section: Introductionmentioning

confidence: 99%

Detection of Hepatitis B Virus Large Surface Protein Using a Time‐Resolved Immunofluorometric Assay

Liu

et al. 2014

Clinical Laboratory Analysis

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“…[11] TRFIA was applied extensively in determination of Canine Creactive Protein (CRP) in serum, [12] Varicella-Zoster Virus (VZV) Immunoglobulin G, [13] hepatitis B virus PreS1 antigen, [14] anticardiolipin antibody IgG. [15] In our article, TRFIA used for the measurement of anti-CCP antibdy in serum with a better accuracy, precision, sensitivity, specificity, and stability performance was proved to be a reliable method.…”

Section: Discussionmentioning

confidence: 92%

Detection of Anti-Cyclic Citrullinated Peptide Using a Time-Resolved Fluoroimmunoassay

Xiao

Liu

et al. 2014

Journal of Immunoassay and Immunochemistry

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In an effort to enhance the linear range of anti-CCP we developed a new immunoassay based on time-resolved fluoroimmunoassay. The precision, sensitivity, specificity, and stability of the assay were evaluated ELISA set as control. The anti-CCP IgG TRFIA kit we established had a wider detectable range than commercial ELISA ones. With regard to intra- and inter-assay precision, the TRFIA kit was better than threee commercial ELISA ones. The mean recovery rate was 101.0%. The TRFIA we developed for anti-CCP IgG detection yielded a more sensitive and reliable method for RA diagnosis and large-scale screening programs as well.

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“…TRFIA is a sophisticated detection technique, and has been widely used in the detection of multiple viruses or their antigens [ 21 , 22 ]. Compared with ELISA, TRFIA has higher sensitivity and wider measurement range.…”

Section: Discussionmentioning

confidence: 99%

A New Method for Detection African Swine Fever Virus: Time-resolved Fluorescence Immunoassay

Chen¹,

Lai²,

Hang³

et al. 2021

J Fluoresc

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African swine fever (ASF) has severely influenced the swine industry of the whole world. Fast and accurate African swine fever virus (ASFV) antigen detection is very important for ASF prevention. This study aims to establish a new detection method for detection ASFV antigen using time-resolved fluorescence immunoassay (TRFIA) in the nose and mouth discharge. A double antibody sandwich TRFIA method was optimized and established. Recombinant P30 recombinant antigen was captured by its antibodies immobilized on 96-well plate, and then banded together with another detection antibodies labeled with Europium(III) (Eu 3+ ) chelates, finally time-resolved analyzer measured the fluorescence intensity. The performance of this TRFIA (sensitivity, specificity and accuracy) was evaluated using the clinical samples and compared with the nucleic acid testing method. The sensitivity of this TRFIA was 0.015 ng/mL (dynamic range 0.24–500 ng/mL) with high specificity. The recovery ranged from 92.00 to 103.62 %, the inter-assay CVs ranged from 5.50 to 11.96 %, and the intra-assay CVs was between 5.20 and 10.53 %. Additionally, the cutoff value was 0.016. TRFIA took only 45 min to generate results, and its detection capability comparable to the nucleic acid detection. This study developed a TRFIA method that could be used for qualitative/quantitative detection of ASFV antigen in pigs nasal discharge, which has high sensitivity, specificity and accuracy. This TRFIA provides a new method for rapidly screening ASFV infection in pigs industry.

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Detection of Hepatitis B Virus Pres1 Antigen Using a Time-Resolved Fluoroimmunoassay

Cited by 14 publications

References 14 publications

Detection of Hepatitis B Virus Large Surface Protein Using a Time‐Resolved Immunofluorometric Assay

Detection of Hepatitis B Virus Large Surface Protein Using a Time‐Resolved Immunofluorometric Assay

Detection of Anti-Cyclic Citrullinated Peptide Using a Time-Resolved Fluoroimmunoassay

A New Method for Detection African Swine Fever Virus: Time-resolved Fluorescence Immunoassay

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