Detection of Helicobacter pylori in Paraffin-Embedded Specimens from Patients with Chronic Liver Diseases, Using the Amplification Method

Abstract

Helicobacter DNA has been detected in the liver specimens of patients with various hepato-biliary diseases. The aim of this study was to investigate the presence of H. pylori DNA in the liver tissue of Iranian patients with chronic liver diseases (CLD). Genomic DNA was extracted from the paraffin sections of 46 liver biopsies of patients with CLD and 13 from patients with metastatic adenocarcinoma as a control group. Polymerase chain reaction (PCR) analysis was carried out using primers for H. pylori 16S rRNA and cagA genes. On analysis, 17 of the 46 patient samples were positive in H. pylori 16S rRNA PCR and 2 of the 13 were positive from the control group. None of the samples were positive for the cagA gene. This study showed the greater presence of H. pylori-like DNA in the liver samples from patients with CLD than in controls.

EFFECT OF HELICOBACTER PYLORI ERADICATION ON HEPATIC STEATOSIS, NAFLD FIBROSIS SCORE AND HSENSI IN PATIENTS WITH NONALCOHOLIC STEATOHEPATITIS: a MR imaging-based pilot open-label study

“…on liver samples of a patient with NASH has been initially reported (9) . This finding was validated by another study, in which the 16S recombinant RNA was found in 5/11 liver samples of NAFLD patients compared with 2/13 controls (17) . However, it remains unclear whether Hp in these specimens is an incidental finding or it has the potential to affect the natural course of NAFLD.…”

“…There are only limited clinical data regarding the association between Hp infection and NAFLD (9,17,19,28) . The presence of 16S recombinant RNA of Hp spp.…”

“…Regarding IR-related morbidity, Hp infection may be implicated in the pathogenesis of obesity and type 2 diabetes mellitus (T2DM) (4,11,27,29) . Limited clinical data also suggest that Hp infection might contribute to the pathogenesis of NAFLD (9,17,28) . We previously showed in an observational study that Hp infection may contribute to the pathogenesis NAFDL (19) .…”

Molecular Epidemiology of Helicobacter pylori in Brazilian Patients with Early Gastric Cancer and a Review to Understand the Prognosis of the Disease

“…In addition, in the last years, H. pylori infection has also been associated with some extradigestive diseases, such as iron-deficiency anemia [20], idiopathic thrombocytopenic purpura [21,22], cardiovascular diseases [23,24], hepatobiliary diseases [25,26], and diabetes mellitus [27], among others.…”

Virulence Factors of Helicobacter pylori and Their Relationship with the Development of Early and Advanced Distal Intestinal Type Gastric Adenocarcinoma

“…In the first case, H. pylori infection is responsible for the development of chronic gastritis, peptic ulcers, mucosa associated lymphoid tissue (MALT lymphoma) and gastric adenocarcinoma (Ahmed & Sechi, 2005). Regarding to extradigestive diseases, H. pylori infection has been associated with iron-deficiency anemia (Capurso et al, 2001), idiopathic thrombocytopenic purpura (Pellicano et al, 2009;Arnold et al, 2009), cardiovascular diseases , hepatobiliary diseases (Isaeva et al, 2009;Pirouz et al, 2009) and amongst others. Especially with regards to gastric cancer, the interest in H. pylori as a cause of this illness began after the pioneering discoveries of Marshall and Warren.…”