Detection of Helicobacter pylori (H. pylori) by histochemical stains in gastric biopsy comparing to immunohistochemistry

Abdalla, Mohamed; Amir, Osman; E, Elnazir Mohammed

doi:10.30574/wjarr.2022.15.2.0793

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…The level of statistical significance was set at P < 0.05. Sensitivity and specificity were calculated based on the following formulae: Sensitivity= (True positive/(True positive + False negative)) * 100%; Specificity= (True negative/(True negative + False positive)) * 100% ( 13 ).…”

Section: Methodsmentioning

confidence: 99%

Improving the detection of Helicobacter pylori in biopsies of chronic gastritis: a comparative analysis of H&E, methylene blue, Warthin-Starry, immunohistochemistry, and quantum dots immunohistochemistry

et al. 2023

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ObjectivesThe objective of the study was to compare the consistency of various staining methods, including H&E, Methylene Blue, Warthin-Starry (W-S), Immunohistochemistry (IHC) and Quantum dots immunohistochemistry (QDs-IHC), in detecting Helicobacter pylori (HP) in cases of mild, moderate and severe chronic gastritis.MethodsBiopsy samples were obtained from 225 patients with chronic gastritis at the Department of Pathology, Yichang Central People’s Hospital between January 2019 and October 2019. The presence of HP was detected using H&E, Methylene Blue, W-S, IHC, and QDs-IHC.ResultsThe positive rates for HP detection using H&E, Methylene Blue, W-S, IHC, and QDs-IHC were 42.22%, 51.11%, 53.78%, 59.11%, and 58.67%, respectively. In cases of mild chronic gastritis, the consistency of test results between H&E, Methylene Blue, W-S, and QDs-IHC with IHC were Kappa=0.196, P=0.033, Kappa=0.706, P<0.001, Kappa=0.717, P<0.001, and Kappa=0.968, P<0.001, respectively. Similarly, in cases of moderate chronic gastritis, Kappa values between H&E, Methylene Blue, W-S, and QDs-IHC with IHC were 0.356, P<0.001, 0.655, P<0.001, 0.741, P<0.001, and 0.946, P<0.001, respectively. For cases of severe chronic gastritis, the Kappa values between the staining methods and IHC were 0.271, P=0.037, 0.421, P=0.002, 0.621, P<0.001, and 1, P< 0.001, respectively.ConclusionThe study showed that the positivity rate of IHC was significantly higher than that of H&E, Methylene Blue, and W-S in detecting HP infection in chronic gastritis cases. In terms of consistency with IHC, QDs-IHC was the most reliable staining method across all severity grades, while the agreement between H&E and IHC was poor, and that between Methylene Blue and W-S with IHC was average. Pathology departments may choose the most appropriate staining method based on their specific needs, considering the staining time, contrast, and cost of each method.

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Section: Methodsmentioning

confidence: 99%

Improving the detection of Helicobacter pylori in biopsies of chronic gastritis: a comparative analysis of H&E, methylene blue, Warthin-Starry, immunohistochemistry, and quantum dots immunohistochemistry

et al. 2023

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“…In the study done by Kocsmár et al, Giemsa revealed an overall sensitivity of 83.3% and a specificity of 98.8%. 20 , 21 Also, PCR can be used for detection of H. pylori using samples from gastric juice, gastric biopsy, dental plaque, saliva, and stools. Moreover, it was reportedly used to find H. pylori in middle ear effusion collected.…”

Section: Introductionmentioning

confidence: 99%

Possible Role of Helicobacter pylori in Ear Nose and Throat Diseases

Aref¹,

Bazeed²,

Nafady³

et al. 2023

IDR

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Background Helicobacter pylori is assumed to cause many gastric and extragastric diseases. We aimed to assess the possible association role of H. pylori in Otitis media with effusion (OME), nasal polyps and adenotonsillitis. Patients and Methods A total of 186 patients with various ear, nose and throat diseases were included. The study comprised 78 children with chronic adenotonsillitis, 43 children with nasal polyps and 65 children with OME. OME patients were assigned to two subgroups: those who have and those who did not have adenoid hyperplasia. Among the patients with bilateral nasal polyps, 20 individuals had recurrent nasal polyps and 23 had de novo nasal polyps. Patients who have chronic adenotonsillitis were divided into three groups: those with chronic tonsillitis and those who underwent tonsillitis, those with chronic adenoiditis and adenoidectomy was performed, and those with chronic adenotonsillitis and underwent adenotonsillectomy. In addition to examination of H. pylori antigen in stool samples of all included patients, real-time polymerase chain reaction (RT-PCR) for detection of H. pylori in the effusion fluid was performed, additionally, Giemsa stain was used for detection of H. pylori organism within the tissue samples when available. Results Frequency of H. pylori in effusion fluid was 28.6% in patients with OME and adenoid hyperplasia, while in those with OME it was only 17.4% with a p value of 0.2. Nasal polyp biopsies were positive in 13% patients of denovo, and 30% patients with recurrent nasal polyps, p=0.2. De novo nasal polyps were more prevalent in the positive stools than recurrent ones, p=0.7. All adenoid samples were negative for H. pylori , only two samples of tonsillar tissue (8.3%) were positive for H. pylori , and stool analysis was positive in 23 patients with chronic adenotonsillitis. Conclusion Lack of association between Helicobacter pylori and occurrence of OME, nasal polyposis or recurrent adenotonsillitis.

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Detection of Helicobacter pylori (H. pylori) by histochemical stains in gastric biopsy comparing to immunohistochemistry

Cited by 2 publications

References 18 publications

Improving the detection of Helicobacter pylori in biopsies of chronic gastritis: a comparative analysis of H&E, methylene blue, Warthin-Starry, immunohistochemistry, and quantum dots immunohistochemistry

Improving the detection of Helicobacter pylori in biopsies of chronic gastritis: a comparative analysis of H&E, methylene blue, Warthin-Starry, immunohistochemistry, and quantum dots immunohistochemistry

Possible Role of Helicobacter pylori in Ear Nose and Throat Diseases

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