Detection of Helicobacter pylori and clarithromycin resistance in gastric biopsies of pediatric patients by using a commercially available real-time polymerase chain reaction after NucliSens semiautomated DNA extraction

Agudo, Sonia; Alarcón, Teresa; Urruzuno, Pedro; Martínez, María Josefa García; López-Brea, M

doi:10.1016/j.diagmicrobio.2010.02.021

Cited by 32 publications

(31 citation statements)

References 20 publications

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“…9 Real-time PCR assay showed sensitivity of 93.33% (negative predictive value, 90.90%) and specificity of 85.95% (positive predictive value, 90.32%). 10 The presence of the genomic material of H pylori within the laryngeal tissue of patients with squamous cell carcinoma of the larynx is a proof of the colonization of the bacterium in that tissue, 11 and biopsy of larynx for real-time PCR may be performed to exclude the possibility of H pylori infection. The larynx may contain diffuse and follicular lymphoid tissue and is documented in the supraglottic region, and most cases are found over the supraglottis.…”

Section: Discussionmentioning

confidence: 99%

Subglottic stenosis induced by extranodal mucosa-associated lymphoid tissue lymphoma

Kuo

Hou

Chu

et al. 2011

Journal of the Chinese Medical Association

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Mucosa-associated lymphoid tissue (MALT) lymphoma is usually associated with a chronic inflammatory disease or autoimmune disorders from which lymphoid tissue of MALT type arises as a prerequisite for lymphoma proliferation. Primary hematopoietic neoplasms of the larynx are rare. MALT lymphomas of the larynx are believed to arise from preexisting or acquired lymphoid tissue of the upper airway which is documented in the supraglottic region. Therefore, these are mainly located in the supraglottic and glottic areas, with only a few reported in the subglottic region. We report on a 50-year-old woman with a hoarseness, stridor, and developing exertional dyspnea. On indirect laryngoscope, multiple nodular lesions with smooth surface over the subglottis with subglottic steonsis was found. The biopsy confirmed the diagnosis of a MALT lymphoma. We hope to promote awareness and consideration of MALT lymphoma in the differential diagnosis in subglottic steonsis.

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Section: Discussionmentioning

confidence: 99%

Subglottic stenosis induced by extranodal mucosa-associated lymphoid tissue lymphoma

Kuo

Hou

Chu

et al. 2011

Journal of the Chinese Medical Association

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“…Nevertheless, culture still remains an important tool as it provides information in terms of antimicrobial susceptibility which is necessary to choose the right treatment option [6,30]. Molecular assays are available to detect DNA point mutations associated with clarithromycin and metronidazole resistance [31,32] but their high price makes it prohibitive for small laboratories to use them. The technique described here could provide a first step to including the detection of DNA mutations associated with antibiotic resistance.…”

Section: Discussionmentioning

confidence: 99%

Utility of normalized genome quantification of Helicobacter pylori in gastric mucosa using an in-house real-time polymerase chain reaction

et al. 2017

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Traditional diagnostic assays for Helicobacter pylori detection have their limitations. Molecular methods can improve both diagnosis and understanding of gastric diseases. Here we describe an in-house quantitative real-time polymerase chain reaction (q-rt-PCR) for the detection of H. pylori in gastric biopsies which has been developed and has a detection limit of 10 copies, the specificity of which was tested against other gastric colonizer bacteria. In this study, 199 gastric biopsies from adults with different clinical gastric symptoms were examined. Biopsies were obtained during endoscopy and the following tests performed: rapid urease testing (RUT), culture and q-rt-PCR. H. pylori bacterial load expressed as bacterial load per 105 cells was calculated using a standard curve. H. pylori was isolated in 41% of patients, RUT was positive in 32% and bacterial genome was detected in 45% (p = 0.010). Concordance between traditional invasive microbiological methods used together and q-rt-PCR was almost 100%. Bacterial load in patients with positive RUT was significantly higher than those where it was negative (p<0.0001). There were also significant differences between bacterial load in patients with more than one positive assay versus those where only one method was positive (p = 0.006). The in-house q-PCR developed here is quick and inexpensive, and allows accurate diagnosis of H. pylori infection. It also permits normalized bacterial load quantification, which is important to differentiate between asymptomatic colonisation and infection.

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“…The A2142G and/or A2143G point mutations Cultured strains containing the A2142G and/or A2143G mutations detected using HelicoDR, 51 PCR-line probe assay, 24 LightCycler PCR, 54 denaturing HPLC (DHPLC) assay, 23 and primer-mismatch PCR. 46,55 The pooled sensitivity and specificity for any of these assays were greater than 0.95, except for GenoType HelicoDR, which had a sensitivity of 89.6%.…”

Section: Performance Of Genotypic Methods In Cultured Strainsmentioning

confidence: 99%

A systematic review and meta‐analysis of genotypic methods for detecting antibiotic resistance in Helicobacter pylori

Wang

et al. 2018

Helicobacter

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Detection of Helicobacter pylori and clarithromycin resistance in gastric biopsies of pediatric patients by using a commercially available real-time polymerase chain reaction after NucliSens semiautomated DNA extraction

Cited by 32 publications

References 20 publications

Subglottic stenosis induced by extranodal mucosa-associated lymphoid tissue lymphoma

Subglottic stenosis induced by extranodal mucosa-associated lymphoid tissue lymphoma

Utility of normalized genome quantification of Helicobacter pylori in gastric mucosa using an in-house real-time polymerase chain reaction

A systematic review and meta‐analysis of genotypic methods for detecting antibiotic resistance in Helicobacter pylori

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