Detection of heavy metal salts with biosensors built with an oxygen electrode coupled to various immobilized oxidases and dehydrogenases

Gayet, Jean-Charles; Haouz, Ahmed; Geloso-Meyer, A.; Burstein, Claude

doi:10.1016/0956-5663(93)85030-r

Cited by 62 publications

(20 citation statements)

References 5 publications

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“…This concentration is the maximum tolerated for mercury compounds in drinking water. It is lower than that obtained with pyruvate oxidase (2 ng/ml) [4] and much lower than those obtained with urease (20 ng/ml [5] % of inhibition and 200 ng/ml [6]). The detection limit of our method is comparable with those of conventional methods such as AAS (2 ng/ml).…”

Section: Calibration Curvescontrasting

confidence: 60%

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Determination of mercury(II), methylmercury and ethylmercury in the ng/ml range with an electrochemical enzyme glucose probe

1995

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Abstract. Hg(II), methylmercury and ethylmercury have been determinedwith an electrochemical glucose probe. Mercury and its compounds inhibit the enzyme invertase which, in presence of its substrate, sucrose, produces glucose. When invertase is in presence of mercury its activity decreases; this causes a decrease of glucose production, which is monitored by the glucose sensor and correlated to the concentration of mercury in solution. Parameters such as pH, enzyme concentration, substrate concentration, and reaction and incubation time were optimized. Results showed that mercury, methylmercury and ethylmercury can be detected directly in aqueous solution in the range 2-10 ng/ml.

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Section: Calibration Curvescontrasting

confidence: 60%

“…Only a few papers concerning the determination of mercury by means of enzymatic biosensors based on amperometric, [4] potentiometric [-5] or thermal [-6] detection have been proposed.…”

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confidence: 99%

Determination of mercury(II), methylmercury and ethylmercury in the ng/ml range with an electrochemical enzyme glucose probe

1995

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“…Because biological components such as enzymes are highly sensitive and selective, the enzyme acid urease was chosen for the determination of mercury and copper. Enzyme systems using ascorbate oxidase and alkaline phosphatase [13], glucoseoxidase [141, urease [15,16], invertase [17] and several dehydrogenases [18], have been reported. Danzer et al [19] have combined three enzyme electrodes (acetylcholinesterase, acid and alkaline phosphatase) for pesticide and heavy metal screening using selected chemometric methods.…”

mentioning

confidence: 99%

“…The toxicity of metals towards fish and luminescent bacteria [20] was also used for a determination of heavy metals. Different transducer systems have been employed: (a) optical [15, 16,21], (b) electrochemical [18,19] and (c) ISFET (ion-selective field effect transistor) [6].…”

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confidence: 99%

The enzymatic determination of mercury and copper using acid urease. The effects of buffers

Preininger

1999

Mikrochim Acta

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Abstract. We report on a quick and simple test based on enzyme inhibition for the detection of mercury and copper using free acid urease coupled to an optical sensor system. Lipophilized Nile Blue was incorporated in plasticized poly(vinyl chloride) (PVC) to produce an ammonium-sensitive layer with a thickness of around 4 gin. The layer was fixed on one side of a disposable cuvette. A solution of buffer, enzyme and heavy metals was placed into the thermostated cell. Enzymatic hydrolysis was started upon addition of urea and the formation of ammonium was monitored. Mercury and copper were the strongest inhibitors; for this reason the inhibitory efficiency of these metals was examined in citrate, acetate and trismaleate buffers. The cuvette test was most sensitive and selective for mercury in a citrate buffer. The limit of detection for mercury(II) ions was as low as 1 gg/L. Copper ions do not interfere because of complexation by citrate. The inhibitory effects of metal combinations on the activity of acid urease and the effects of optimum pH of the enzyme and the transducer on the dynamic range of the cuvette test are presented.

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“…Heavy metals can also be determined with ion-sensitive electrodes; however, the electrode selectivity and response cannot be associated with the toxicity impact of those chemicals. Therefore, enzyme sensors have been studied and constructed for determination of inhibitors (Gayet et al, 1993;Cowell et al, 1995); they are based essentially on the inhibition of purified enzymes like urease, cholinesterase, and cytochrome oxidase (Tran-Minh, 1985;Albery et al, 1990). To provide inexpensive biosensors for environmental control, the enzyme purification step needs to be avoided and enzymes are kept as such in their natural system to ensure a better lifetime (Samson and Popovic, 1988;Rechnitz and Ho, 1990).…”

Section: Introductionmentioning

confidence: 99%

Optical Algal Biosensor using Alkaline Phosphatase for Determination of Heavy Metals

Durrieu

Tran‐Minh

2002

Ecotoxicology and Environmental Safety

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A biosensor is constructed to detect heavy metals from inhibition of alkaline phosphatase (AP) present on the external membrane of Chlorella vulgaris microalgae. The microalgal cells are immobilized on removable membranes placed in front of the tip of an optical fiber bundle inside a homemade microcell. C. vulgaris was cultivated in the laboratory and its alkaline phosphatase activity is strongly inhibited in the presence of heavy metals. This property has been used for the determination of those toxic compounds.

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Detection of heavy metal salts with biosensors built with an oxygen electrode coupled to various immobilized oxidases and dehydrogenases

Cited by 62 publications

References 5 publications

Determination of mercury(II), methylmercury and ethylmercury in the ng/ml range with an electrochemical enzyme glucose probe

Determination of mercury(II), methylmercury and ethylmercury in the ng/ml range with an electrochemical enzyme glucose probe

The enzymatic determination of mercury and copper using acid urease. The effects of buffers

Optical Algal Biosensor using Alkaline Phosphatase for Determination of Heavy Metals

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