Detection of HCV RNA in the Peripheral Blood Mononuclear Cells of Serum HCV RNA-Negative Egyptian Patients Under Interferon Treatment

Zayed, Rania A.; Rushdy, Eman Ahmed; Saleh, Doa’a A.

doi:10.1097/maj.0b013e3181ec435e

Cited by 15 publications

(10 citation statements)

References 21 publications

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“…Transmission from mothers to infants is greater in mothers with identified positive strands of HCV RNA in their PBMCs, than those with HCV RNA negative strands (20). Taking into account the undetectability of HCV RNA in the newborns' plasmas, and the important role of PBMCs in HCV infections (21,22), we performed a real-time PCR assay on the newborns' PBMCs to detect HCV RNA and to investigate the role of these cells in HCV RNA negative newborns. As the results show, HCV RNA was not detected in the cells, nor was it found in the plasma.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Hepatitis C Virus Infection in Antibody Positive Orphan Newborns

Ziyaeyan

Jamalidoust

Moeini

2012

Jundishapur J Microbiol

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Comparing diagnostic methods of vertical transmission of HCV infection can serve as a cornerstone for better understanding of the capability of each methods in more accurate diagnosis of HCV infection in the newborns.HCV is spread through contaminated blood and blood products with percutaneous exposure. This disease also poses a risk to injection drug users (IDU) and hemodialysis patients. However, sexual transmission of HCV is comparably low in Iran, and in addition the risk through occupational exposure is also very low. Mother-to-infant transmission rates vary widely (5-9). Usually, a mother BackgroundHepatitis C virus (HCV) infection is a major cause of; chronic hepatitis, liver cirrhosis and liver cancer. It represents an enormous global health problem, affecting 130-170 million people worldwide. In Iran, the prevalence of HCV in the general population is 0.5% (1), but the rate is higher in certain at risk populations (2-4).Background: The rate of mother-to-infant hepatitis C virus (HCV) transmission is relatively low, however, this mode of infection is the most important route for childhood HCV involvement. The chances of transmission are increased by the level of maternal HCV viraemia and maternal human immunodeficiency virus (HIV) co infection. Objectives: The objective of the present study was to determine the presence of HCV infection in orphan newborns of infected mothers. Patients and Methods: A total of 29, two to seven month old, orphan infants were included in the study. The tests performed consisted of the detection of; anti-HCV and anti-HIV antibodies, HCV RNA in the plasma and peripheral blood mononuclear cells by realtime polymerase chain reaction (PCR), and finally the detection of HCV core antigens (HCV core Ag). Results: Anti-HCV antibodies were detectable in all of the infants. However HCV RNA was undetectable in both plasma and peripheral blood mononuclear cells (PBMCs) and there were also no anti-HIV 1/2 antibodies present in the infants. Conclusions: Few studies have addressed the issue of mother-to-infant transmission of HCV among orphan newborns. According to the present study, carried out with orphan babies, the use of a HCV core Ag assay and evaluation of HCV RNA in PBMCs together with HCV RNA real-time PCR on the plasma, could benefit the prediction or exclusion of HCV transmission from mother to child.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Hepatitis C Virus Infection in Antibody Positive Orphan Newborns

Ziyaeyan

Jamalidoust

Moeini

2012

Jundishapur J Microbiol

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“…During the follow-up, 80% of the patients, who became serum HCV positive 6 months after the end of treatment had detectable level of HCV RNA in PBMCs at the end of treatment. Zayed et al (15) concluded that the absence of HCV in the serum of patients by the end of treatment did not exclude future viremia. The patient might still be a source of infection to others.…”

Section: Discussionmentioning

confidence: 99%

“…These results were similar to that of the current study. Zayed et al (15) aimed at investigating the predictive value of HCV RNA in PBMCs of 70 patients with chronic hepatitis C after 48 weeks of IFN treatment, which may act as the source of HCV reinfection of hepatic cells. They revealed the presence of detectable HCV RNA in the PBMCs of 27% of patients, despite clearance of serum HCV RNA.…”

Section: Discussionmentioning

confidence: 99%

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Assessment of Hepatitis C Virus RNA in Peripheral Blood Mononuclear Cells as a Predictor of Response to Pegylated-Interferon and Ribavirin: A Cohort Study

Miri

Sharafi

Salimi

et al. 2017

Iran Red Crescent Med J

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Background: Sustained virologic response (SVR) to pegylated-interferon (PegIFN) and ribavirin (RBV) in hepatitis C virus (HCV)-infected patients could be predicted by detection of serum HCV RNA whereas detection of HCV RNA in other reservoirs such as peripheral blood mononuclear cells (PBMCs) for prediction of treatment response is still a mystery. Objectives: This study aimed at assessing the prediction of SVR by detection of HCV RNA in PBMCs or serum in patients during treatment. Methods: In a cohort study (2011 to 2014), 100 chronic HCV patients at Tehran Hepatitis Center were treated with PegIFN and RBV. Serum HCV RNA level was measured at baseline, 4, 12, and 24 weeks during treatment and at 24 weeks after termination of treatment. Meanwhile, HCV RNA was evaluated in PBMCs at weeks 4, 12, and 24 during the treatment. Results: Out of 100 patients treated in this study, 91 completed the course of treatment. Most patients were young males infected with HCV genotype 1. Cirrhosis and previous history of treatment was found in 16.5% and 26.5% of patients. Sustained virologic response was achieved in 65 (71.4%) patients. Among baseline parameters, only female gender was significantly associated with SVR. Undetectable serum HCV RNA at week 4 (OR = 4.74) and week 12 (OR = 11.63) of treatment predicted SVR rate while the same was not true for detection of serum HCV RNA at week 24 of treatment. Moreover, detection of HCV RNA in PBMCs at weeks 4 and 12 of treatment was not associated with the rate of SVR, while absence of HCV RNA in PBMCs at week 24 of treatment was associated with SVR (OR = 4.55). Conclusions: Detection of HCV RNA in PBMCs, especially at week 24 of treatment with PegIFN and RBV, could be considered as an additional marker for prediction of treatment response. It is recommended to assess HCV on-treatment kinetic in PBMCs of patients treated with direct-acting antiviral agents for prediction of treatment response.

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“…The estimated number of humans infected with the virus reaches 170 million worldwide including 0.7 million in Poland (Flisiak et al 2011; Zagożdżon et al 2009). Although HCV is a primary hepatotropic pathogen, its replication has been demonstrated in other cells, most notably in peripheral blood mononuclear cells (PBMC) and macrophages (Azzari et al 2008; Chary et al 2012; Laskus et al 2000, 2004; Page et al 2010; Revie and Salahuddin 2011; Zayed et al 2010). …”

Section: Introductionmentioning

confidence: 99%

Seronegative Hepatitis C Virus Infection

Kaźmierczak

Cortés

Radkowski

2013

Arch. Immunol. Ther. Exp.

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Hepatitis C virus (HCV) is a major cause of liver disease worldwide. The routine diagnostics identifying HCV infection include testing for specific anti-HCV antibodies by enzyme-linked immnunosorbent assay and viral genetic material in serum or plasma. However, a small proportion of patients persistently infected with HCV, in whom anti-HCV are undetectable, constitute a serious diagnostic and possibly epidemiologic problem, as they could facilitate pathogen spread in the population. This type of infection is termed seronegative or serosilent. Seronegative HCV infection is currently of great interest to both scientists and physicians. The review presents epidemiological data concerning the prevalence of seronegative HCV infection in HIV/HCV co-infected individuals, hemodialysis patients, and blood and organ donors. The possible mechanisms behind this atypical course of infection are discussed. Furthermore, the differences between seronegative and occult infections and prolonged seroconversion are explained.

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Detection of HCV RNA in the Peripheral Blood Mononuclear Cells of Serum HCV RNA-Negative Egyptian Patients Under Interferon Treatment

Cited by 15 publications

References 21 publications

Evaluation of Hepatitis C Virus Infection in Antibody Positive Orphan Newborns

Evaluation of Hepatitis C Virus Infection in Antibody Positive Orphan Newborns

Assessment of Hepatitis C Virus RNA in Peripheral Blood Mononuclear Cells as a Predictor of Response to Pegylated-Interferon and Ribavirin: A Cohort Study

Seronegative Hepatitis C Virus Infection

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