Detection of harmful foodborne pathogens in food samples at the points of sale by MALDT-TOF MS in Egypt

Abstract

Objectives Microbes can contaminate foodstuffs resulting in foodborne illnesses. Investigating microbial hazards in foods at the point of sale with rapid tools is required to avoid foodborne illness outbreaks. The current study aimed to identify the microbial hazards in food samples collected from retail shops at sale points using MALDI-TOF MS. Results Food samples were collected from stores and supermarkets in four Delta cities (Tanta, Kutour, Kafr-Elzayat and Benha). Analysis of 178 samples of fish, meat and dairy products revealed 20 different bacterial species. 44.76% of isolates were identified as E. coli, 17.44% were identified as Enterobacter spp., and E. cloacae was predominant. 12.2% were identified as Citrobacter spp., and C. braakii was predominant, and 8.7% were identified as Klebsiella spp., and K. pneumoniae was predominant. Moreover, eight Proteus mirabilis, six Morganella morganii, five Staphylococcus hominis, three Serratia marcescens, two Pseudomonas aeruginosa, one Salmonella typhimurium and one Enterococcus faecalis were detected. Foodstuffs not only be contaminated during production and processing but also during storage and transport. Identification of harmful human pathogens in foodstuffs is alarming and consider threatening to public health. Identification of microbiological hazards in foods using MALDI-TOF MS provides an efficient tool for identifying foodborne pathogens.

WGS-Based Phenotyping and Molecular Characterization of the Resistome, Virulome and Plasmid Replicons in Klebsiella pneumoniae Isolates from Powdered Milk Produced in Germany

“…All isolates were re-identified using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) at the species level with a log value > 2.300. Protein extraction from pure colonies of each sample was done as previously described [ 22 ]. The MALDI-TOF measurements were carried out using a Microflex LT instrument (Bruker Daltonics, Bremen, Germany), following the MALDI Biotyper manufacturer’s recommendation on the log score value of 0–3 for species identification.…”

Isolation and Identification of major bacteria from three Ethiopian rift valley lakes live and processed fish, and water samples: Implications in sanitary system of fish products

“…Salmonella spp and fecal coliforms (E. coli, K. pneumoniae and E. aerogenes) bacteria were detected in 42% of water samples and 64% of processed sh samples. This result is in agreement with several studies which reported the isolation of E. coli, Enterobacter spp., and Citrobacter spp., and Klebsiella spp., P. aeruginosa, and Salmonella from sh products (Sha et al, 2020 andKhater et al, 2021).This was indicative of post contamination of sh product in the processing system, fecal pollution and poor quality of processed sh which is linked with the practice of inadequate hygienic measure, mishandling, improper storage and use of dirty water during processing and all unhygienic condition of the processes.…”

Compostos Bioativos Em Frutos De Acerolas Submetidos Ao Branqueamento Por Vapor E Congelamento

“…Apesar dessa relação de concordância mais elevada em alguns grupos bacterianos, o método MALDI-TOF o qual é baseado na determinação da massa proteica bacteriana, apresenta maior acurácia na detecção de enterobactérias que apresentam resultados indeterminados nos testes convencionais, além de apresentar um banco de dados vasto que informa o grau de confiabilidade do resultado, tornando o processo de identificação mais rápido e preciso (CHERKAOUI et al, 2010;PAVLOVIC et al, 2013;PERUZY et al, 2019). Desse modo, a técnica MALDI-TOF torna-se um método mais confiável para a identificação de diferentes grupos bacterianos em alimentos, sendo possível a identificação de microrganismos patogênicos, deteriorantes e associados a ecologia microbiana (ELBEHIRY et al, 2017;ALTAKHIS et al, 2021;KHATER et al, 2021), bem como de marcadores associados a resistência a antibióticos (OVIAÑO et al, 2017).…”