Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil

Silva, Mariana S.A.; Uzêda, Rosângela Soares; Costa, Kattyanne Souza; Santos, Sara Lima; Macedo, Alan Caine Costa de; Abe‐Sandes, Kiyoko; Gondim, Luís Fernando Pita

doi:10.1016/j.vetpar.2009.02.007

Cited by 27 publications

(21 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Also, N. caninum DNA was found in brains of goats from BA. Silva et al (2009) analyzed brains, hearts and tongues of 102 goats from slaughterhouses, and found a frequency of 1, 96% (2/102), using primers for ITS-1 region (JS4/CT2b; CT1/CT2).…”

Section: Epidemiologymentioning

confidence: 99%

All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

Neospora caninum is protozoan parasite with domestic and wild dogs, coyotes and grey wolves as the definitive hosts and many warm-blooded animals as intermediate hosts. It was cultivated and named in 1988. Neosporosis is a major disease of cattle and has no public health significance. Since 1990's N. caninum has emerged as a major cause of abortion in cattle worldwide, including in Brazil. N. caninum also causes clinical infections in several other animal species. Considerable progress has been made in understanding the biology of N. caninum and there are more than 200 papers on this subject from Brazil. However, most of the reports on neosporosis from Brazil are serological surveys. Overall, little is known of clinical neosporosis in Brazil, particularly cattle. The few reports pertain to sporadic cases of abortion with no information on epidemics or storms of abortion. The objective of the present review is to summarize all reports from Brazil and suggest topic for further research, including prevalence of N. caninum oocysts in soil or in canine feces, and determining if there are additional definitive hosts, other than the domestic dog. There is need for a national survey in cattle using defined parameters. Future researches should focus on molecular characterization of N. caninum strains, possibility of vaccine production and relationship between wildlife and livestock epidemiology.Keywords: Neospora caninum, neosporosis, domestic animals, wild animals, Brazil. ResumoNeospora caninum é um protozoário parasita que possui os canídeos domésticos e selvagens, coiotes e lobos cinzentos como hospedeiros definitivos e vários animais de sangue quente como hospedeiros intermediários. Foi cultivado e nomeado em 1988. A neosporose é uma das principais doenças em bovinos e não tem significância em saúde pública. Desde 1990, N. caninum tem emergido como uma das principais causas de aborto em bovinos em todo o mundo, inclusive no Brasil. N. caninum também causa infecções clínicas em várias outras espécies animais. Consideráveis avanços foram feitos na compreensão da biologia desse parasita e há mais de 200 trabalhos sobre o assunto no Brasil. No entanto, a maioria dos relatos de neosporose do Brasil são relacionados a sorologia. Em geral, pouco se sabe sobre a neosporose clínica no Brasil, particularmente em bovinos. Os poucos relatos referem-se a casos esporádicos de aborto sem informações sobre epidemias ou surtos de aborto. O objetivo da presente revisão é resumir todos os relatos sobre N. caninum no Brasil e sugerir tópicos para pesquisas futuras, incluindo a prevalência de oocistos de N. caninum no solo ou em fezes caninas e determinar se há hospedeiros definitivos adicionais, exceto o cão doméstico no país. Uma pesquisa nacional em bovinos usando parâmetros definidos seria de grande importância. Pesquisas futuras deveriam ser focadas na caracterização de cepas de N. caninum, possibilidade de produção de vacinas e a relação epidemiológica entre a vida selvagem e o gado.Palavras-chave: Neospora caninum, neospo...

show abstract

Section: Epidemiologymentioning

confidence: 99%

All about neosporosis in Brazil

Cerqueira‐Cézar

Calero‐Bernal

Dubey

et al. 2017

Rev. Bras. Parasitol. Vet.

View full text Add to dashboard Cite

show abstract

“…DNA was extracted from a 50 µm thick section of formalin-fixed, paraffinembedded cerebrum using a Maxwell16 automated extractor and FFPE Plus LEV DNA Purification Kit (Promega). Initial nested PCR was performed using 2 pairs of primers complementary to portions of the nu clear ribosomal internal transcribed spacer 1 (ITS1) sequences conserved among Hammondia spp., Neo spora caninum and T. gondii as previously described (Silva et al 2009). When this was initially unsuccessful, a semi-nested protocol targeting a shorter se quence, more likely to be successful with DNA damaged by formalin fixation, was designed.…”

Section: Polymerase Chain Reaction Sanger Sequencing and Blastn Analmentioning

confidence: 99%

“…When this was initially unsuccessful, a semi-nested protocol targeting a shorter se quence, more likely to be successful with DNA damaged by formalin fixation, was designed. The first round used primers CT1 (5'-TGA ATC CCA AGC AAA ACA-3') and CT2 (5'-GCG CGA GCC AAG ACA TCC AT-3') and was amplified as previously described (Silva et al 2009). The second round used novel primer ToxHamNeoITS1F (5'-TTG CCT TCT TCA TGT TGG AT-3') with CT2 and was amplified using conditions identical to the first round.…”

Section: Polymerase Chain Reaction Sanger Sequencing and Blastn Analmentioning

confidence: 99%

Disseminated toxoplasmosis Toxoplasma gondii in a wild Florida manatee Trichechus manatus latirostris and seroprevalence in two wild populations

Smith

Waltzek²,

Rotstein³

et al. 2016

Dis. Aquat. Org.

View full text Add to dashboard Cite

Marine mammals are important indicators for ecosystem health and serve as sentinel species for infectious agents including zoonoses. Histological examination of tissues from a stranded Florida manatee Trichechus manatus latirostris revealed protozoal cysts in the cerebrum and intrahistiocytic tachyzoites in the liver and caudal mesenteric lymph node. Disseminated Toxoplasma gondii infection was confirmed by immunohistochemistry and sequencing of the nuclear ribosomal internal transcribed spacer region of formalin-fixed tissues. The lack of baseline information on Florida manatees' exposure to this pathogen prompted a study into the seroprevalence of T. gondii in 2 separate geographic habitats in Florida, USA, during the winters from 2011−2014. Serum was collected during routine health assessments of 44 apparently healthy manatees from Crystal River (n = 26) on the west central coast of Florida and Brevard County (n = 18) on the east coast of Florida. Serum was screened for detection of T. gondii immunoglobulin G (IgG) antibodies via the modified agglutination test. Two animals from Crystal River from 2011 and 2012 (7.7%) and one animal from Brevard County from 2011 (5.6%) tested positive for T. gondii antibodies. Overall seroprevalence for T. gondii was low in the 2 sampled populations and may reflect a low seroprevalence or animal susceptibility. However, continued monitoring of this pathogen in aquatic ecosystems is warranted due to both possible anthropogenic sources and zoonotic potential.

show abstract

“…Serolojik testlerden ELISA testi daha hızlı sonuç vermesi sebebiyle özellikle çok sayıda hayvanın serolojik analizinin yapılmasında daha fazla tercih edilmektedir [9][10][11][12] .…”

Section: Makale Kodu (Article Code): Kvfd-2011-4724unclassified

Seroprevalence of Neospora caninum in Goats in Niğde Province

Cayvaz¹,

Karatepe²

2009

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

ÖzetBu çalışma, Şubat 2010 -Mayıs 2010 tarihleri arasında Niğde ilinde bulunan keçilerde Neospora caninum'un seroprevalansının araştırılması amacı ile yapılmıştır. Çalışmanın materyali olarak Niğde ilinde 8 farklı çalışma merkezine ait (Bahçeli, Merkez-Tepeköy, Bor, Çamardı Celaller Köyü, Kızılca, Çukurkuyu, Ulukışla, ve Çamardı Merkez) 1 yaş ve üzeri toplam 181 dişi keçi belirlenmiş ve bu keçilerin serumlarında N. caninum antikorları ELISA testi ile araştırılmıştır. Niğde yöresinde ELISA testi ile incelenen toplam 181 keçinin 47 (%25.9)'sinin N. caninum antikorları yönünden seropozitif olduğu tespit edilmiştir. Serolojik incelemede, en fazla seropozitiflik %42.3 oranı ile 5 yaşındaki keçilerde tespit edilirken en az seropozitiflik oranı ise %13.8 ile 3 yaşındaki keçilerde belirlenmiştir. Abort yapan 16 keçinin 6 (%37.5)'sı, abort yapmayan 165 keçinin 41 (%24.8)'i seropozitif olarak tespit edilmiştir. Ayrıca çalışma merkezleri arasında en yüksek seropozitiflik %54.5 oranı ile Çamardı'da belirlenmiş, buna karşılık Bahçeli ve Kızılca'da ise muayene edilen keçilerde seropozitiflik saptanmamıştır. Elde edilen seropozitiflik oranlarında; keçilerin yaş grupları ve çalışma merkezlerinin istatistiksel olarak önemli (sırası ile P<0.05, P<0.01) olduğu, abort yapan ve yapmayan keçiler arasında ise önemsiz olduğu saptanmıştır (P>0.05). Sonuç olarak, Niğde yöresinde keçilerde N. caninum'un varlığı ilk kez bu çalışma ile ortaya konulmuş ve abort olaylarında neosporosisin de dikkate alınması gerektiği kanısına varılmıştır. Anahtar sözcükler: Neospora caninum, Keçi, Niğde, Seroprevalans Seroprevalence of Neospora caninum in Goats in Niğde Province SummaryThis study was performed to investigate seroprevalence of Neospora caninum in goats in Niğde province between February 2010-May 2010. A total of 181 goats which were one year old or more than one year old, were selected from eight different study sites (Bahçeli, Centrum-Tepeköy, Bor, Çamardı Celaller Village, Kızılca, Çukurkuyu, Ulukışla, ve Central Çamardı) as the study sample and N. caninum antibodies were tested in these goats serum using ELISA. The 47 out of 181 sera (25.9%) were found to be seropositive for N. caninum. In the serologic examinations, the seropositivity rate was highest in 5 years old (42.3%) while it was lowest in 3 years old (13.8%) in goats. The 6 (37.5%) out of 16 aborted goats and 41 (24.8%) out of 165 non-aborted were detected as seropositive. The highest seropositivity rate of study sites was determined in Çamardı as 54.5% in addition to this, none of examined goats in Bahçeli and Kızılca detected seropositivity. The difference between the seropositivity rates to N .caninum among age groups and study sites was statistically significant (P<0.05, P<0.01, respectively) while the difference to N. caninum among aborted and non-aborted goats was statistically insignificant (P>0.05). As a result, this is the first serologic survey for N. caninum performed on goats in Niğde province and neosporosis should be taken into consideration in abortion cases.

show abstract

Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil

Cited by 27 publications

References 36 publications

All about neosporosis in Brazil

All about neosporosis in Brazil

Disseminated toxoplasmosis Toxoplasma gondii in a wild Florida manatee Trichechus manatus latirostris and seroprevalence in two wild populations

Seroprevalence of Neospora caninum in Goats in Niğde Province

Contact Info

Product

Resources

About