Detection of hair-microRNAs as the novel potent biomarker: Evaluation of the usefulness for the diagnosis of scleroderma

Wang, Zhongzhi; Jinnin, Masatoshi; Kudo, Hideo; Inoue, Kuniko; Nakayama, Wakana; Honda, Noritoshi; Makino, Katsunari; Kajihara, Ikko; Fukushima, Satoshi; Inoue, Yoshio; Ihn, Hironobu

doi:10.1016/j.jdermsci.2013.06.018

Cited by 39 publications

(29 citation statements)

References 22 publications

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“…2a-c), which is consistent with the previous report (9). Accordingly, hair miR-19a has different expression pattern from that in the skin or serum and can be an independent biomarker.…”

Section: Resultssupporting

confidence: 91%

“…We also reported that the hair miRNA levels did not correlate with serum miRNA levels (9), suggesting that they can be independent biomarkers. We hypothesized that hair miRNA levels may be novel disease markers for SSc.…”

Section: Question Addressedmentioning

confidence: 73%

“…Hair samples (five pieces, about 5 cm in length) were obtained from the occiput of each individual of 20 patients with SSc (two men and 18 women; age range, 50-85) (9). Control hair samples were collected from 13 age-and sex-matched volunteers.…”

Section: Experimental Designmentioning

confidence: 99%

“…RNA was extracted from the hair shafts by Isohair and Isogen (Nippon Gene, Toyama, Japan) (9). RNA extraction from sera or skin section and cDNA synthesis were performed as described previously (6,9,10).…”

Section: Experimental Designmentioning

confidence: 99%

“…RNA extraction from sera or skin section and cDNA synthesis were performed as described previously (6,9,10). Primers for miR-29a or keratin 34 were obtained from Takara (Shiga, Japan).…”

Section: Experimental Designmentioning

confidence: 99%

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Hair miR‐29a levels are decreased in patients with scleroderma

Takemoto

Jinnin

Wang

et al. 2013

Experimental Dermatology

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In the present study, we evaluated the possibility that we can utilize hair shaft miR-29a levels as disease marker of scleroderma. Hair samples were obtained from 20 scleroderma patients, five dermatomyositis patients and 13 controls. microRNAs were purified from hairs as well as skins or sera, and miR-29a levels were measured with quantitative real-time polymerase chain reaction. Mean hair miR-29a levels in scleroderma patients were significantly lower than those in control subjects or dermatomyositis, while expression levels of hair shaft marker keratin 34 were similar among them. There was no strong correlation among the miR-29a levels in the hair, skin and serum of each patient, suggesting that hair microRNAs can be independent biomarkers. We found scleroderma patients with decreased miR-29a levels had contracture of the phalanges at a significantly higher prevalence than those without. To confirm the clinical usefulness of hair microRNAs, large-scale researches are needed in the future.

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“…2a-c), which is consistent with the previous report (9). Accordingly, hair miR-19a has different expression pattern from that in the skin or serum and can be an independent biomarker.…”

Section: Resultssupporting

confidence: 91%

Section: Question Addressedmentioning

confidence: 73%

Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

“…RNA extraction from sera or skin section and cDNA synthesis were performed as described previously (6,9,10). Primers for miR-29a or keratin 34 were obtained from Takara (Shiga, Japan).…”

Section: Experimental Designmentioning

confidence: 99%

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Hair miR‐29a levels are decreased in patients with scleroderma

Takemoto

Jinnin

Wang

et al. 2013

Experimental Dermatology

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Essential Role of microRNA in Skin Physiology and Disease

Glavač

Ravnik‐Glavač

2015

Advances in Experimental Medicine and Biology

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The identification and characterization of microRNAs (miRNAs) is a rapidly growing area of research also in dermatology. Skin represents the largest organ in the human body, and its morphogenesis has been shown to require a highly coordinated and undisrupted miRNA profile. High expression of several miRNAs in the epidermis and hair follicles is necessary for normal skin development. Profiling studies have identified numerous differentially regulated miRNAs associated with either normal physiological status of the skin or some pathological processes or both. This chapter covers current knowledge of the important roles of miRNAs in the pathogenesis of some skin diseases including systemic lupus erythematosus (SLE), systemic sclerosis (SSc), dermatomyositis (DM), psoriasis (PS), and skin cancer, especially malignant melanoma (MM). In addition, the diagnostic and therapeutic relevance of miRNAs that are involved in pathological processes of the skin are elucidated providing further information for some possible clinical implications especially for their use as therapeutic targets or disease biomarkers.

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Epigenetics

Jinnin¹

2016

Systemic Sclerosis

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Detection of hair-microRNAs as the novel potent biomarker: Evaluation of the usefulness for the diagnosis of scleroderma

Cited by 39 publications

References 22 publications

Hair miR‐29a levels are decreased in patients with scleroderma

Hair miR‐29a levels are decreased in patients with scleroderma

Essential Role of microRNA in Skin Physiology and Disease

Epigenetics

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