Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

Georges, Karla; Loria, Guido Ruggero; Riili, S; Greco, Anna; Caracappa, Santo; Jongejan, Frans; Sparagano, Olivier

doi:10.1016/s0304-4017(01)00488-5

Cited by 200 publications

(119 citation statements)

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“…Recently, the first molecular evidence of Ap infection in a sick dog living in the south of Italy [22] and two sick dogs living in northern Greece [25] were reported. There is also one study that detected Ap DNA in blood samples from cattle in Sicily [13] and another study in which Ap antibodies were reported (5.7%) in free-ranging jackals in Israel [44].…”

Section: Discussionmentioning

confidence: 99%

“…In the Mediterranean basin I. ricinus is rarely encountered [9]. Further evidence supporting infrequent exposure to the Ixodes species in the Mediterranean basin [4,13] is the fact that Lyme disease is uncommonly reported in dogs or humans in this region. As described in this study, the overall Bb seroprevalence was only 0.6% and there was not a statistical association between Bb and Ap seroreactivity among the three study sites.…”

Section: Discussionmentioning

confidence: 99%

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A serological study of exposure to arthropod-borne pathogens in dogs from northeastern Spain

Solano‐Gallego

Llull

Osso³

et al. 2006

Vet. Res.

103

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-There is limited information regarding the prevalence of many vector borne pathogens in Europe and especially in Spanish dogs. We investigated 206 sick and 260 clinically healthy dogs from three different regions in northeastern Spain for antibodies to Rickettsia conorii (Rc), Ehrlichia canis (Ec), Anaplasma phagocytophilum (Ap), Bartonella henselae (Bh), Bartonella vinsonii subsp. berkhoffii (Bvb), Leishmania infantum (Li) and Borrelia burgdorferi (Bb) and for antigen of Dirofilaria immitis (Di). Total prevalences were the following: Rc (56.4%), Li (30%), Ec (16.7%), Bh (16.8%), Ap (11.5%), Bvb (1.07%), Di (0.6%) and Bb (0.6%). Seroprevalences for Rc, Ec, Ap, Bh, and Bvb and Bb and Di antigens were similar among the three different study sites. The Ec seroprevalence, as determined by Snap 3DX, was statistically lower in dogs from Mallorca (0%) than Tarragona (16%) and Barcelona (5%) (P < 0.0001). Detection of Rc antibodies was associated with seroreactivity to Ec and Ap antigens (P = 0.018 and P = 0.002, respectively). IFA Ec antibodies were associated with Ap seroreactivity (P < 0.0001). There was no association between the clinical status, sex, time of the year when samples were collected, life-style or exposure to fleas or ticks and a positive test result for Ec, Bh, Bvb, or Bb antibodies or Di antigens. Li seroreactivity was associated with illness and living outdoors (P < 0.0001, P = 0.029; respectively), Rc seroreactivity with the male gender (P = 0.028) and Ap seroreactivity with living outdoors (P = 0.045). This study indicates that exposure to Rc, Li, Ec or related Ehrlichia spp., Bh and Ap or a related spp., is common whereas Di, Bb and Bvb is uncommon among dogs from the Mediterranean basin. We also provide serological data that suggests the existence of a novel Ehrlichia species on Mallorca island. rickettsial pathogens / dogs / Spain

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A serological study of exposure to arthropod-borne pathogens in dogs from northeastern Spain

Solano‐Gallego

Llull

Osso³

et al. 2006

Vet. Res.

103

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“…The reverse line blot (RLB) method (Gubbels et al 1999), based on simultaneous detection of the 18S rRNA gene for various Babesia and Theileria species, has been extensively used for surveillance purposes (Georges et al 2001; Almerìa et al 2002;Oura et al 2004;Nijhof et al 2003Nijhof et al , 2005Altay et al 2007;M'ghirbi et al 2008;Altay et al 2008;Matjila et al 2008;Oosthuizen et al 2008; Almerìa et al 2009;Salih et al 2010;Yusufmia et al 2010; Tomassone et al 2012;Ceci et al 2014;Githaka et al 2014;Eygelaar et al 2015;Njiiri et al 2015). The method relies on the amplification of the V4 hypervariable region of the 18S rRNA gene, followed by hybridization to probes for various The current study aimed to assess whether other genotypes may be amplified by the primers used in these assays, since it was shown that multiple templates may affect sensitivity of the diagnostic assays (Pienaar et al 2011a).…”

Section: Introductionmentioning

confidence: 99%

Investigating the diversity of the 18S SSU rRNA hyper-variable region of Theileria in cattle and Cape buffalo (Syncerus caffer) from southern Africa using a next generation sequencing approach

Mans

Pienaar

Ratabane

et al. 2016

Ticks and Tick-borne Diseases

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Molecular classification and systematics of the Theileria is based on the analysis of the 18S rRNA gene. Reverse line blot or conventional sequencing approaches have disadvantages in the study of 18S rRNA diversity and a next-generation 454 sequencing approach was investigated. The 18S rRNA gene was amplified using RLB primers coupled to 96 unique sequence identifiers (MIDs). Theileria positive samples from African buffalo (672) and cattle (480) from southern Africa were combined in batches of 96 and sequenced using the GS Junior 454 sequencer to produce 825711 informative sequences. Sequences were extracted based on MIDs and analysed to identify Theileria genotypes. Genotypes observed in buffalo and cattle were confirmed in the current study, while no new genotypes were discovered.Genotypes showed specific geographic distributions, most probably linked with vector distributions. Host specificity of buffalo and cattle specific genotypes were confirmed and prevalence data as well as relative parasitemia trends indicate preference for different hosts.Mixed infections are common with African buffalo carrying more genotypes compared to cattle. Associative or incongruent co-infection profiles were observed between genotypes that may have implications for speciation and systematics: specifically that more Theileria species may exist in cattle and buffalo than currently recognized. Analysis of primers used for Theileria parva diagnostics indicate that no new genotypes will be amplified by the current primer sets confirming their specificity. Theileria parva SNP variants that occur in the 18S rRNA hypervariable region were confirmed. A next generation sequencing approach is useful in obtaining comprehensive knowledge regarding 18S rRNA diversity and prevalence for the Theileria, allowing for the assessment of systematics and diagnostic assays based on the 18S gene.Keywords: 18S SSU, Diversity, Next-generation sequencing, species, Theileria, Theileria parva 3 IntroductionThe Babesia and Theileria are part of the phylum Apicomplexa (Order Piroplasmorida) (Mans et al. 2015 A significant proportion of the systematic study of the Piroplasmorida comprise analysis of the 18S rRNA gene (Allsopp et al. 1994;Chae et al. 1999;Chansiri et al. 1999;Criado-Fornelio et al. 2004;Reichard et al. 2005;Criado et al. 2006;Altay et al. 2007;Bhoora et al. 2009). The reverse line blot (RLB) method (Gubbels et al. 1999), based on simultaneous detection of the 18S rRNA gene for various Babesia and Theileria species, has been extensively used for surveillance purposes (Georges et al. 2001; Almerìa et al. 2002;Oura et al. 2004;Nijhof et al. 2003Nijhof et al. , 2005Altay et al. 2007;M'ghirbi et al. 2008;Altay et al. 2008;Matjila et al. 2008;Oosthuizen et al. 2008; Almerìa et al. 2009;Salih et al. 2010;Yusufmia et al. 2010; Tomassone et al. 2012;Ceci et al. 2014;Githaka et al. 2014;Eygelaar et al. 2015;Njiiri et al. 2015). The method relies on the amplification of the V4 hypervariable region of the 18S rRNA gene, followed by hybridization t...

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“…and Theileria spp. (Georges et al, 2001). All tested samples were amplified twice, once with the Anaplasma spp.…”

Section: Dna Extractionmentioning

confidence: 99%

Ticks and Tick-Borne Pathogens From Wildlife in the Free State Province, South Africa

Tonetti

Berggoetz

Rühle

et al. 2009

Journal of Wildlife Diseases

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ABSTRACT:Eight ixodid tick species, associated with 59 free-ranging mammals belonging to 10 species, were collected at five different localities in the Free State Province, South Africa. Four of the study areas were nature reserves (Willem Pretorius, Sandveld, Tussen-die-Riviere, and Soetdoring), and one site was a private farm located in Senekal district. The collection was performed from March 2006 until June 2006. Ticks (n5569) and tissues from animals (n552) were analyzed by polymerase chain reaction, reverse line blot, and sequencing for various tickborne pathogens belonging to the genera Babesia, Theileria, Anaplasma, and Ehrlichia. Rhipicephalus (Boophilus) microplus, the known vector of Babesia bovis responsible for Asiatic redwater in South Africa, was found for the first time in the Free State Province. Rhipicephalus appendiculatus also was collected in areas in the Free State where it has not been previously described. Anaplasma marginale was detected for the first time in a gemsbok (Oryx gazella gazella). Gene sequences recovered in this study were 98-100% homologous with GenBank sequences for Anaplasma bovis, Theileria separata, and Theileria sp. Malelane sable antelope.

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Detection of haemoparasites in cattle by reverse line blot hybridisation with a note on the distribution of ticks in Sicily

Cited by 200 publications

References 16 publications

A serological study of exposure to arthropod-borne pathogens in dogs from northeastern Spain

A serological study of exposure to arthropod-borne pathogens in dogs from northeastern Spain

Investigating the diversity of the 18S SSU rRNA hyper-variable region of Theileria in cattle and Cape buffalo (Syncerus caffer) from southern Africa using a next generation sequencing approach

Ticks and Tick-Borne Pathogens From Wildlife in the Free State Province, South Africa

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