Detection of H2O2 release from vascular endothelial cells

Panus, Peter C.; Radí, Rafael; Chumley, Phillip; Lillard, Robert H.; Freeman, Bruce Α.

doi:10.1016/0891-5849(93)90013-k

Cited by 94 publications

(49 citation statements)

References 22 publications

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“…The cells were then transferred to a TM quartz flat cell, and EPR spectra were Hydrogen peroxide determination. Extracellular H 2 O 2 released from MCF-7 cells was measured by a fluorometric assay using pHPA in the presence of HRP, as previously described (22). Briefly, this method used the fact that H 2 O 2 reacts with HRP-forming compound I, which in turn reacts with pHPA, forming a stable fluorescent dimer, [pHPA] 2 .…”

Section: Methodsmentioning

confidence: 99%

Manganese Superoxide Dismutase Modulates Hypoxia-Inducible Factor-1α Induction via Superoxide

et al. 2008

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Hypoxia-inducible factor 1 (HIF-1) is a transcription factor that plays an important role in O 2 homeostasis. Numerous observations suggest that changes in reactive oxygen species affect HIF-1A stabilization and HIF-1A transcriptional activation in many cell types. The antioxidant enzyme manganese superoxide dismutase (MnSOD) modulates the cellular redox environment by converting superoxide (O 2 .À ) to hydrogen peroxide and dioxygen. Previous results from our group have shown that overexpression of MnSOD in MCF-7 cells alters stabilization of HIF-1A under hypoxic conditions; however, the underlying mechanism(s) is not known. Here, we tested the hypothesis that MnSOD regulates the expression of HIF-1A by modulating the steady-state level of O 2.À

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Section: Methodsmentioning

confidence: 99%

Manganese Superoxide Dismutase Modulates Hypoxia-Inducible Factor-1α Induction via Superoxide

et al. 2008

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“…Cells (2 x 105) grown in 35-mm Petri dishes were exposed to 50 ~mol/1 HzO 2 in PBS (2 ml/ dish) at 37 ~ HzOz-degradation by HIT, RIN and J-774 cells was assayed by measuring the fluorescence produced by pHPA through H2Oz-dependent oxidation of pHPA to its fluorescent dimer [31]. The degradation of H~O 2 was followed for up to 60rain.…”

Section: Methodsmentioning

confidence: 99%

Insulinoma cells in culture show pronounced sensitivity to alloxan-induced oxidative stress

1995

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SummaryIn a model system of cultured J-774 cells we have previously shown that alloxan in moderate concentrations is toxic only in the presence of a reducing agent with the production of hydrogen peroxide. The cytotoxicity was found to involve lysosomal destabilization. In the present study the cytotoxic effects of (i) alloxan alone, (ii) a combination of alloxan and cysteine or (rio hydrogen peroxide were investigated in two established insulinoma cell lines (HIT-T15 and RINm5F), and compared with the effects on J-774 cells. The protective effects of desferrioxamine and catalase, and the intracellular levels of reduced glutathione and activities of the enzymes glutathione peroxidase, glutathione reductase and catalase were also studied. HIT and RIN cells showed about 10 times greater sensitivity than J-774 cells against exposure to either alloxan and cysteine, or hydrogen peroxide. All cell types were relatively insensitive to alloxan alone. Preincubation with desferrioxamine and addition of catalase provided efficient protection against cytotoxicity and lysosomal destabilization. HIT and RIN cells had less capacity to degrade hydrogen peroxide and lower levels of glutathione peroxidase than J-774 cells. The lysosomal stability in all three cell lines was directly correlated to their viability. We conclude that HIT and RIN cells have weak antioxidative defence systems resulting in enhanced lysosomal vulnerability when they are exposed to alloxan and cysteine, which produce hydrogen peroxide extracellularly. The degree of cytotoxicity seems to be dependent on cellular capacity to degrade hydrogen peroxide and the lysosomal content of reactive iron. [Diabetologia (1995) 38: 635-641]

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“…For example, fluorescence of the (PHPA) 2 probe used in ROS measurement will increase with pH (Panus et al, 1993), while pH in the medium of C.…”

Section: Nutrients and Ros Productionmentioning

confidence: 99%

Effects of nutrients, salinity, pH and light:dark cycle on the production of reactive oxygen species in the alga Chattonella marina

Wenhua

Anderson

et al. 2007

Journal of Experimental Marine Biology and Ecology

127

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Experiments were carried out to investigate the effects of nutrients, salinity, pH and light:dark cycle on growth rate and production of reactive oxygen species (ROS) by Chattonella marina, a harmful algal bloom (HAB) species that often causes fish kills. Different nitrogen forms (organic-N and inorganic-N), N:P ratios, light:dark cycles and salinity significantly influenced algal growth, but not ROS production.However, iron concentration and pH significantly affected both growth and ROS production in C. marina. KCN (an inhibitor of mitochondrial respiration) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (an inhibitor of photosynthesis) had no significant effects on ROS production. Vitamin K 3 (a plasma membrane electron shuttle) enhanced ROS production while its antagonist, dicumarol, decreased ROS production. Taken together, our results suggest that ROS production by C. marina is related to a plasma membrane enzyme system regulated by iron availability but is independent of growth, photosynthesis, availability of macronutrients, salinity and irradiance.

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Detection of H2O2 release from vascular endothelial cells

Cited by 94 publications

References 22 publications

Manganese Superoxide Dismutase Modulates Hypoxia-Inducible Factor-1α Induction via Superoxide

Manganese Superoxide Dismutase Modulates Hypoxia-Inducible Factor-1α Induction via Superoxide

Insulinoma cells in culture show pronounced sensitivity to alloxan-induced oxidative stress

Effects of nutrients, salinity, pH and light:dark cycle on the production of reactive oxygen species in the alga Chattonella marina

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