Detection of H-2 alleles by PCR-RFLP—an alternative to flow cytometry in the screening of transgenic mice

Klebs, Sven; Hoffmann, Matthias; Musholt, Petra B.; Bayer, Bettina; Musholt, Thomas J.

doi:10.1016/s0022-1759(03)00063-2

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Genetically Matched Pluripotent Embryonic Stem Cells Generat...1

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2007

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“…We employed PCR amplification followed by allelespecific restriction enzyme digestion or direct sequencing of single nucleotide polymorphisms (SNP) within the H-2 region of chromosome 17 to determine if recombination had restored heterozygosity at the MHC loci (13). 24 out of 72 p(MII)ES cells (33%) harbored the heterozygous MHC genotype of the oocyte donor (Supporting Online Material Fig.…”

Section: Genetically Matched Pluripotent Embryonic Stem Cells Generat...mentioning

confidence: 99%

Histocompatible Embryonic Stem Cells by Parthenogenesis

Kim

Lerou

Yabuuchi

et al. 2007

Science

207

188

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Genetically matched pluripotent embryonic stem (ES) cells generated via nuclear transfer or parthenogenesis (pES cells) are a potential source of histocompatible cells and tissues for transplantation. After parthenogenetic activation of murine oocytes and interruption of meiosis I or II, we isolated and genotyped pES cells and characterized those that carried the full complement of major histocompatibility complex (MHC) antigens of the oocyte donor. Differentiated tissues from these pES cells engrafted in immunocompetent MHC-matched mouse recipients, demonstrating that selected pES cells can serve as a source of histocompatible tissues for transplantation.

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Section: Genetically Matched Pluripotent Embryonic Stem Cells Generat...mentioning

confidence: 99%