Detection of group B Streptococcus (GBS) in pregnant women by SYBR Green real-time PCR

Abstract

Objectives: Application of SYBR Green real-time PCR and bacterial isolation methods to detect Streptococcus agalactiae (GBS) carriage in women at 35-37 weeks’ gestation. Patients and Method: Use of SYBR Green real-time PCR and bacterial isolation methods to detect GBS in 116 women at 35 - 37 weeks’ gestation. Results: The rate of carrier of GBS in women at 35 - 37 weeks gestation was 9.5% (11 pregnant women), in which real-time PCR method was positive in all positive GBS women, while bacterial culture method was positive at 6% (7 pregnant women). These methods (culture and real-time PCR) had substantial agreement with the value of Kappa is 0,77. Checking for the targeted sequence amplification of real-time PCR by the curve of melting temperature and agarose electrophoresis of amplified product, the real-time PCR product was correctly targeted at the expected genetic sequence. Conclusion: SYBR Green real-time PCR method for detecting GBS in pregnant women is useful, low-cost and easy for performing. Therefore, it is suitable for detecting GBS in diagnostic laboratories where real-time PCRs are available. Key words: Streptococcus agalactiae (GBS), real-time PCR, pregnant woman

Aerobic Vaginitis in the Third Trimester and Its Impact on Pregnancy Outcomes

“…It must be pointed that the presence of this species does not mean AV diagnosis. Our previous study revealed 6% and 9.5% when a culture or SYBR Green real-time PCR technique was applied to 116 pregnant women to detect GBS, respectively [20]. In the present study, culture revealed this species from 10 women (3%) from the 323 cohort of pregnant women.…”