Detection of granulocyte colony-stimulating factor and its receptor in human follicular luteinized granulosa cells

Salmassi, Ali; Schmutzler, A. G.; Li-li, Huang; Hedderich, Jürgen; Jonat, W.; Mettler, Liselotte

doi:10.1016/j.fertnstert.2003.09.039

Cited by 72 publications

(50 citation statements)

References 32 publications

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“…At the level of the reproductive tract, G-CSF has been previously shown to be secreted by granulosa cells at ovulation (Salmassi et al, 2004), then within the endometrium during the luteal phase, and finally during gestation in the placenta (Duan, 1990). More recently, a randomized controlled trial reported that G-CSF administration significantly increases live-birth rates in patients with unexplained recurrent miscarriages which failed to respond to a previous immunotherapy treatment (Scarpellini and Sbracia, 2009).…”

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confidence: 99%

New pre-conception immune biomarkers for clinical practice: interleukin-18, interleukin-15 and TWEAK on the endometrial side, G-CSF on the follicular side

Lédée

Petitbarat

Rahmati

et al. 2011

Journal of Reproductive Immunology

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a b s t r a c tIdentification of biomarkers of optimal uterine receptivity to the implanting embryo as well as biomarkers of oocyte competence would undoubtedly improve the efficiency of assisted reproductive technology (ART). Expression of IL-15 and IL-18 has been shown to be different in patients with failed implantation after IVF/ICSI compared with fertile controls and both correlate with local uNK (CD56+) recruitment and angiogenesis. Tumor necrosis factor weak inducer of apoptosis (TWEAK) has been described in mice as a potent early immune regulator able to protect the conceptus. The results of our studies in human suggest that TWEAK modulates the IL-18 related cytotoxicity of uNK cells. Quantification of IL-18, TWEAK and IL-15 mRNA expression by real-time PCR in endometrial tissue collected in midluteal phase of non-conception cycles allowed documentation of physiological events that occur at the time of uterine receptivity. Such information may be useful for the physician especially in patients where embryos fail to implant. Cytokine quantification may assist in understanding the mechanisms leading to repeated IVF/ICSI failure: either depletion of cytokines necessary for the apposition-adhesion, or an excess of cytokines leading to local cytotoxicity, may impair the implantation of the embryo. Other new data suggest that a pre-conception dialogue mediated by the oocyte and the follicular fluid and the oocyte may contribute to later implantation success. Follicular concentration of G-CSF appears as a useful biomarker of oocyte competence before fertilization. Moreover both in human and animal models, evidence of a role of the endometrium as a biosensor of the embryo is emerging.

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confidence: 99%

New pre-conception immune biomarkers for clinical practice: interleukin-18, interleukin-15 and TWEAK on the endometrial side, G-CSF on the follicular side

Lédée

Petitbarat

Rahmati

et al. 2011

Journal of Reproductive Immunology

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“…Follicular granulosa cells seem to represent one of the sources and targets of G-CSF (12). Since cytokines preferentially act locally, the source of FF G-CSF located nearest the oocyte, could have a major influence on the development of the oocyte.…”

Section: Cumulus Oophorus T Cells: a Source Of Ff G-csfmentioning

confidence: 99%

“…Its main role is to act on the proliferation, differentiation and activation of haematopoietic cells of the neutrophilic lineage. G-CSF is also secreted by cells of the reproductive tract: human luteinized follicular granulosa cells (12), endometrial cells (13), and cells from decidual, placental (14) and various foetal tissues (15). At ovulation, G-CSF concentrations are much higher in FF than in serum (6).…”

Section: Introductionmentioning

confidence: 99%

“…At ovulation, G-CSF concentrations are much higher in FF than in serum (6). Studies using western blot and immunohistochemistry techniques have located the G-CSF protein and its receptor in the ovary, mainly in the granulosa cells of the follicle and in luteal cells (12). Previous studies of G-CSF in serum provided some evidence of its involvement in implantation.…”

Section: Introductionmentioning

confidence: 99%

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Cumulus oophorus T cells: a possible source of follicular fluid G-CSF, biomarker predictive for embryo implantation in IVF/ICSI cycles

Piccinni¹

2015

CCE

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SummaryGranulocyte-colony stimulating factor (G-CSF) in individual follicular fluid (FF) may provide a non-invasive biomarker of implantation to be evaluated, to select the oocyte and hence the embryo with the highest implantation potential to transfer in IVF/ICSI (In Vitro Fertilization/ Intracytoplasmatic Sperm Injection) cycles. We wonder what could be the source of FF-G-CSF. Since cytokines preferentially act locally, the source of FF G-CSF could be located nearest the oocyte. Cumulus oophorus surrounds the oocyte and then in physiological pregnancy surrounds, breaking up progressively, the derived embryo during the first 72 hours until its implantation. Thus, cumulus could have a strong influence particularly on oocyte and then on embryo development and quality. We found that cumulus T cells produce G-CSF, which could be: a) the source of FF G-CSF induced by IL-4 produced by T cells in response to cumulus-derived progesterone and b) responsible together with cumulus derived-progesterone for the cumulus T cells IL-4 production, which in turn could induce the production of LIF by these T cells. Cumulus T cell-derived LIF, which increases the quality of embryos, may provide a microenvironment suitable for embryo pre-implantation development and may explain the increased implantation rate of the embryos associated with high levels FF G-CSF.

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“…G-CSF has been shown to be secreted by granulosa cells at ovulation (Salmassi et al, 2004), then during the luteal phase within the endometrium and finally during gestation in the placenta (Duan, 1990). FF G-CSF may promote local maternofetal tolerance (Rutella et al, 2005) or influence the oocyte's own mRNA levels or its potential for self-repair (Yannaki et al, 2005).…”

mentioning

confidence: 99%

The actors of human implantation: gametes, embryo and endometrium

Gridelet¹,

Gaspard²,

Polese³

et al. 2012

Embryology - Updates and Highlights on Classic Topics

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Detection of granulocyte colony-stimulating factor and its receptor in human follicular luteinized granulosa cells

Cited by 72 publications

References 32 publications

New pre-conception immune biomarkers for clinical practice: interleukin-18, interleukin-15 and TWEAK on the endometrial side, G-CSF on the follicular side

New pre-conception immune biomarkers for clinical practice: interleukin-18, interleukin-15 and TWEAK on the endometrial side, G-CSF on the follicular side

Cumulus oophorus T cells: a possible source of follicular fluid G-CSF, biomarker predictive for embryo implantation in IVF/ICSI cycles

The actors of human implantation: gametes, embryo and endometrium

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