Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method

Endo, Shigeo; Komori, Takashi; Ricci, Giannina; Sano, Ayako; Yokoyama, Koji; Ohori, Akira; Kamei, Katsuhiko; Franco, Marcello; Miyaji, Makoto; Nishimura, Kazuko

doi:10.1016/j.femsle.2004.03.015

Cited by 47 publications

(46 citation statements)

References 16 publications

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“…3A). This sensitivity is comparable to that of standard PCR and similar to values (10 to 100 fg) mentioned by other authors for their LAMP-based systems (7,17,34). However, the LAMP detection limit was only 1 pg when C. krusei PYCC 3341 T genomic DNA was used (Fig.…”

Section: Vol 46 2008 Identification Of Clinically Relevant Yeast Spsupporting

confidence: 88%

“…For instance, we obtained better results in the LAMP reaction with a 90-min incubation at 64°C than with incubation for a standard period of 60 min or less. Some authors would corroborate this result (7,8,36), while others would disagree (29). Another possibility for increasing LAMP sensitivity and accelerating the response time would be the additional utilization of loop primers in the reaction mixture (31).…”

Section: Discussionmentioning

confidence: 87%

“…There has been a clear emphasis on the diagnosis of viral and bacterial infections (8,11,13,16,17,35,37,42), but parasites such as Plasmodium falciparum (36) and Trypanosoma spp. (19,44) and pathogenic fungi such as Paracoccidioides brasiliensis (7) and Ochroconis gallopava (34) have also been addressed. LAMP-based approaches have been applied to a wide range of samples, such as paraffin-embedded tissues (7), whole blood (36), nasopharyn-geal swabs (17,40), dental plaques (23), eggs (13), and potato leaf samples (32).…”

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confidence: 99%

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Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

2008

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The occurrence of invasive mycoses has progressively increased in recent years. Yeasts of the genus Candida remain the leading etiologic agents of those infections. Early identification of opportunistic yeasts may contribute significantly to improved disease management and the selection of appropriate antifungal therapy. We developed a rapid and reliable molecular identification system for clinically relevant yeasts that makes use of nonspecific primers to amplify a region of the 26S rRNA gene, followed by reverse hybridization of the digoxigenin-labeled products to a panel of species-specific oligonucleotide probes arranged on a nylon membrane macroarray format. DNA amplification was achieved by the recently developed loop-mediated isothermal DNA amplification technology, a promising option for the development of improved laboratory diagnostic kits. The newly developed method was successful in distinguishing among the major clinically relevant yeasts associated with bloodstream infections by using simple, rapid, and cost-effective procedures and equipment.

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Section: Vol 46 2008 Identification Of Clinically Relevant Yeast Spsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 87%

mentioning

confidence: 99%

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Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

2008

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“…An accurate and rapid diagnosis system for periodontitis is essential for periodontal treatment. Several recent reports have demonstrated the usefulness of the LAMP method (10,11,17,26,27,33,49). Therefore, we focused on the LAMP method for the rapid detection of periodontopathic bacteria.…”

Section: Discussionmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic BacteriaPorphyromonas gingivalis,Tannerella forsythia, andTreponema denticola

et al. 2005

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Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method, was developed for the rapid detection of the major periodontal pathogens Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola. The LAMP method amplifies DNA with high specificity, efficiency, and rapidity under isothermal conditions using a set of four specially designed primers and a DNA polymerase with strand displacement activity. In this study, we initially designed the primers for LAMP assays to detect these bacteria and evaluated the specificity and sensitivity of these assays. The specificities of the primers for these bacteria were examined using various oral bacteria and various reaction times. The lower detection limits of the 60-min LAMP reaction without loop primers were 1 g/tube for P. gingivalis, 10 fg/tube for T. forsythia, and 1 ng/tube for T. denticola. Addition of the loop primers for each bacterium improved the detection specificities and sensitivities by several magnitudes. Furthermore, LAMP assays were applied to the rapid detection of these periodontal pathogens in clinical specimens, and the results were compared with those of conventional PCR detection. The results of the LAMP assays corresponded to those of conventional PCR assays. These results indicate that the LAMP assay is an extremely rapid, highly sensitive, specific method. This method is very useful for the rapid detection of periodontopathic bacteria and the diagnosis of periodontal disease.

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“…Each aliquot of 200 µL of swab specimens was tested by the LAMP method, as described above. On the other hand, aliquots of 20 methods are available Endo et al, 2004;Kawano et al, 2015;Ohori et al, 2006;Uemura et al, 2008;Yo et al, 2016 . In this study, we developed a LAMP method using a primer set for detecting a wide range of fungi from environmental specimens to evaluate the fungal burden, which should help to prevent allergies or infections caused by fungi.…”

Section: Detection Of Fungi In the Indoor Environmentmentioning

confidence: 99%

Detection of Fungi from an Indoor Environment using Loop-mediated Isothermal Amplification (LAMP) Method

Nakayama¹,

Yamazaki²,

Yo³

et al. 2017

Biocontrol Sci.

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Loop-mediated isothermal amplification LAMP is a useful DNA detection method with high specificity and sensitivity. The LAMP reaction is carried out within a short time at a constant temperature without the need for thermal cycling. We developed a LAMP primer set for detecting a wide range of fungi by aligning the sequences of the large subunit ribosomal RNA gene of Candida albicans Ascomycota , Cryptococcus neoformans Basidiomycota , and Mucor racemosus Mucorales . The threshold of C. albicans rDNA as template with our LAMP primer set was in the range of 10-100 copies per a reaction. In this study, we evaluated the correlation between colony forming units CFU and LAMP detection rate using the LAMP method for environmental fungi. The LAMP method should be a useful means of detecting fungi in indoor environments, disaster areas, or even in confined manned spacecraft to prevent allergies or infections caused by fungi.

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Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method

Cited by 47 publications

References 16 publications

Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

Efficient Identification of Clinically Relevant Candida Yeast Species by Use of an Assay Combining Panfungal Loop-Mediated Isothermal DNA Amplification with Hybridization to Species-Specific Oligonucleotide Probes

Loop-Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic BacteriaPorphyromonas gingivalis,Tannerella forsythia, andTreponema denticola

Detection of Fungi from an Indoor Environment using Loop-mediated Isothermal Amplification (LAMP) Method

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