Detection of GMO in food products in Brazil: the INCQS experience

Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings

“…To this purpose, a primer amplifying a fragment of 447 bp of the p-35S/CP4 EPSPS gene (Cardarelli et al, 2005) was used. In 57% of the samples that were found positive for smaller transgenic fragment, such sequence was detected (see addendum).…”

Detection of genetically modified maize events in Brazilian maize-derived food products

“…The quencher dye In Brazil, some studies have attempted to detect transgenic material in food product containing maize even before the first marketing authorization of genetically modified maize in 2007. Cardarelli et al (2005) did not detect MON810 and Bt176 maize in pasta and pet food samples. Dinon, De Melo and Arisi (2008) and Dinon, Bosco and Arisi (2010) did not find Bt11, Bt176 and MON810 either in maize flour, corn meal, maize flour flakes, and polenta samples sold in Brazilian markets from 2005 to 2007.…”

“…4860 of April 2003(BRASIL, 2003) to ensure that consumers are properly informed about the presence of genetically modified ingredients in the food products. Since then, some studies on the detection and quantification of levels of genetically modified soybean and maize in Brazilian food products have been published (BRANQUINHO; FERREIRA; CARDARELLI-LEITE, 2010;BROD et al, 2007;BROD;CARDARELLI et al, 2005;DINON et al, 2010;FERREIRA;BRANQUINHO;CARDARELLI-LEITE, 2009;GREINER;KONIETZNY, 2008;MARCELINO;GUIMARÃES;BARROS, 2007). The methods used are based on the Polimerase Chain Reaction (PCR), which is the analytical system widely adopted in GMO detection, and the quantifications are based on real-time PCR (qPCR) that targets different genetic elements, including the cauliflower mosaic virus 35S promoter (P-35S) ( VAN DEN EEDE, 2010).…”

Detection and quantification of Roundup Ready® soybean residues in sausage samples by conventional and real-time PCR

“…In Brazil, growers were first authorized to sell RR® soybean in . In 2004and 2005, both cultivation and commercialization of RR® soybean were authorized by the Brazilian government. As GM soybean growth increased in the country, the presence of GM residues in human and animal food proportionally rose.…”

“…The DNA-based methods make use of the polymerase chain reaction (PCR) technique and can be applied both to products in natura and to highly processed foods. However, some products cause many inconveniences for extracting DNA with satisfactory amplificability (VAITILINGOM et al, 1999;LIPP et al, 2001;HÜBNER;STUDER;LÜTHY, 1999;VOLLENHOFER et al, 1999;CARDARELLI et al, 2005).…”