Detection of Glycosylated Hemoglobin on Boronic Acid-Modified Zeolitic Imidazolate Framework-8 Nanoparticles

Lakhera, Praveen; Chaudhary, Vikas; Singh, Sukhbir; Vishwakarma, Neelam; Sanchez Huertas, Cesar; Kumar, Parveen; Kumar, Sanjeev

doi:10.1021/acsanm.3c04054

Cited by 3 publications

(2 citation statements)

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“…Following the pH screening, a new set of Hb solutions was prepared, this time in a pH 6.0 buffer solution. The absorbance of each Hb solution in the pH 6.0 buffer was measured using the UV–vis spectrophotometer at the established wavelength of 406 nm with the contribution of isosbestic point of Hb . A second calibration line was constructed by plotting the absorbance values against the corresponding concentrations (mg/mL) of the Hb solutions in the pH 6.0 buffer ( y = 0.1571 x + 0.0019 ( R 2 = 0.9987).…”

Section: Resultsmentioning

confidence: 99%

“…The absorbance of each Hb solution in the pH 6.0 buffer was measured using the UV–vis spectrophotometer at the established wavelength of 406 nm with the contribution of isosbestic point of Hb. 55 A second calibration line was constructed by plotting the absorbance values against the corresponding concentrations (mg/mL) of the Hb solutions in the pH 6.0 buffer ( y = 0.1571 x + 0.0019 ( R 2 = 0.9987). These calibration lines serve as reference tools, establishing a quantitative relationship between absorbance values and the concentration of Hb solutions.…”

Section: Resultsmentioning

confidence: 99%

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Selective Extraction and Quantification of Hemoglobin Based on a Novel Molecularly Imprinted Nanopolymeric Structure of Poly(acrylamide-vinyl imidazole)

Şarkaya,

Özçelik,

Yaşar

et al. 2024

ACS Omega

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Imbalances in hemoglobin (Hb) levels can lead to conditions such as anemia or polycythemia, emphasizing the importance of precise Hb extraction from blood. To address this, a novel synthetic imprinted polymer was meticulously developed for capturing and separating Hb. Poly(acrylamide-vinylimidazole) nanopolymer (poly(AAm-VIM)) was synthesized using acrylamide and vinyl imidazole as functional monomers through surfactant-free emulsion polymerization. Characterization using FTIR, particle size, zeta potential, and SEM ensured the polymer's structure. The Hb-imprinted nanopolymer (Hbpoly(AAm-VIM)) demonstrated notable specificity, with a calculated Hb-specific adsorption value (Q max ) of 3.7377 mg/g in a medium containing 2.5 mg/mL Hb. The molecularly imprinted polymer (MIP) exhibited approximately 5 times higher Hb adsorption than the nonimprinted polymer (NIP). Under the same conditions, the imprinted nanopolymer displayed 2.39 and 2.17 times greater selectivity for Hb over competing proteins such as bovine serum albumin (BSA) and lysozyme (Lys), respectively. Also, SDS-PAGE analysis results confirmed the purification of Hb by the molecularly imprinted nanopolymer. These results underscore the heightened specificity and efficacy of the molecularly imprinted nanopolymer in selectively targeting Hb atoms among other proteins. Incorporating such polymers is justified by their notable affinity, cost-effectiveness, and facile production. This research contributes valuable insights into optimizing synthetic imprinted polymers for efficient Hb extraction, with potential in medical diagnostics and treatment applications.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%