Detection of glutathione transferase activity on polyacrylamide gels

A bentazone-resistant mutant of cyanobacterium, Synechococcus elongatus PCC7942 adapts different strategies to counteract on bromoxynil- and salt-mediated oxidative stress

“…For GST the gel was incubated for 10 min each in 0.1 MK-PO 4 buffer pH 6.5 and 0.1 MK-PO 4 buffer pH 6.5 containing 4.5 mM GSH, 1 mM CDNB and 1 mM NBT, followed by 0.1 M TrisHCl buffer pH 9.6 containing 3 mM phenazine methosulphate. Achromatic zones were discernable against dark blue background (Ricci et al 1984).…”

Evaluation of Phytoremediation Potential of <i>Catharanthus roseus</i> with Respect to Chromium Contamination

“…The sites on the gel where the enzyme peroxidase was present were stained blue which after sometime turned brown confirming the presence of native peroxidase. Gels were stained for GST activity after native PAGE using the method of Ricci et al [23]. Blue insoluble formazan appeared on the gel surface in about 3 -5 min, except in the GST area.…”

Pre-treatment of Fulvic Acid Plays a Stimulant Role in Protection of Soybean (Glycine max L.) Leaves Against Heat and Salt Stress

“…GST ISOENZYME ACTIVITY Equal amounts of protein were separated by 10% (w/v) native PAGE according to the method described by Laemmli (1970) and stained for GST activity according to Ricci et al (1984). Briefly, after electrophoresis, the gel was equilibrated in 0.1 M potassium phosphate buffer (pH 6.5) for 10 min and transferred to a reaction mixture containing 4.5 mM GSH, 1 mM CDNB and 1 mM nitroblue tetrazolium in 0.1 M potassium phosphate buffer (pH 6.5) at 37 o C for 10 min.…”