Detection of GES-2, a Class A β-Lactamase Produced by Pseudomonas aeruginosa in a Teaching Hospital in Iran

Abstract

Background: Guiana Extended-Spectrum (GES-2) as one of the Extended-spectrum β-lactamases (ESBLs), produced by Pseudomonas aeruginosa, is able to compromise the effectiveness of imipenem and tends to be geographically limited. Objectives: The aim of this study was to detect the spread of GES-2 β-lactamase gene among ESBL-producing P. aeruginosa isolates in a teaching hospital in Kashan, Iran. Materials and Methods: Detection of β-lactamase gene blaGES-2 in eight ESBL producing isolates of P. aeruginosa collected from 100 clinical and environmental specimens was performed by PCR method. The susceptibility of the isolates to eight antibacterial agents was determined by disk diffusion method. ESBL production of the isolates was detected by double disk synergy test. DNA sequencing and aligning with the reference sequence were performed using BLAST and CLUSTAL W for selected PCR products of the blaGES-2 gene. Results: A total of 100 environmental and clinical isolates of P. aeruginosa were collected. Eight of the isolates were found to be ESBL positive. The highest resistance rate was detected for piperacillin (75%), while the lowest resistance rate was for ciprofloxacin (12.5%). Fifty percent of the isolates (four out of eight) were imipenem-susceptible and MDR. The blaGES-2 gene was detected in all ESBL-producing isolates. Sequencing of both forward and reverse strands of the blaGES-2 gene were identified by BLASTn search as the ATP-binding cassette (ABC) transporter permease, partial cds, clone G-1 P. aeruginosa (Accession no. AB591379.1). Conclusions:According to the present data, this is the first report on the presence of blaGES-2 gene of ESBL-producing P. aeruginosa from teaching hospitals of Iran. Fifty percent of the blaGES-2 positive P. aeruginosa isolates were multi-drug resistance (MDR).

Antibiotic susceptibility pattern of Pseudomonas aeruginosa expressing blaGES and blaPER genes in two different hospitals

“…The gene, blaGES-1, conferred an extendedspectrum cephalosporin resistance profile, including clavulanic acid (CA), tazobactam and imipenem (IPM). Generally, there are different GES variants, some are ESBL and some are carbapenemases and the integron genetic framework of GES is the essential factor that develops resistance to broad-spectrum β-lactam antibiotics and other dissimilar classes of antimicrobials (Tavajjohi et al, 2013;Weldhagen et al, 2003;Frase et al, 2011).…”

Molecular study of Acinetobacter baumannii Isolated from skin infection

“…baumannii isolates were furthers exanimated to determine the occurrence of ( blaSHV, blaTEM, blaNDM-1) genes responsible on some antibiotic resistance for B-lactam antibiotic. The result of blaSHV manifested from 8 A. baumannii isolates, only 4 isolates showed the positive result for blaSHV gene as in figure( 6) that represent 40% of total number of isolates while represent 44.4% of 9 isolates that have ESBLs and the result of blaTEM manifested from 8 of A. baumannii isolates, only 7 isolates showed the positive result for blaTEM gene as in figure (7) that represent 70% of total number of isolates while represent 77.7% of 9 isolates that have ESBLs While blaNDM not show any result and are negative in all isolates. The results showed that the genetic detection of antibiotic resistance genes in A. baumannii were the A1,A3 and A5 contain both blaTEM and blaSHV genes while other isolates contain either blaTEM or blaSHV.…”

“…A. baumannii was produced ESBLs(extended spectrum β-lactamases) that mediate resistance to extended spectrum cephalosporins (ESCs) (7). NDM , IMP and VIM genes responsible for MBLs production are horizontally transferable via plasmids and can rapidly spread to other bacteria.…”