Detection of genomic variability in different populations of the cattle tick Boophilus microplus in southern Brazil

Passos, Daniel Thompsen; Ferreira, Carlos Alexandre Sanchez; Silva, S.S. da; Richter, Marc François; Ozaki, Luiz Shozo

doi:10.1016/s0304-4017(99)00147-8

Cited by 19 publications

(7 citation statements)

References 26 publications

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“…Our results lead to the suggestion that geographic isolation would not be a single determining cause of the variation in the bm86 gene, because the seven strains that were identical are from geographic regions different in climate, vegetation and relief. This contrasts with some authors who have suggested geographic isolation as the main cause of genetic variation in B. microplus (Garcı´a-Garcı´a et al 1990Baxter and Barker 1998;Hernandez et al 1998Hernandez et al , 2000Passos et al 1999;De La Fuente et al 2000;Guerrero et al 2001).…”

Section: Strainscontrasting

confidence: 74%

Polymorphism of the bm86 Gene in South American Strains of the Cattle Tick Boophilus microplus

et al. 2005

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Thirty Boophilus microplus strains from various geographic regions of Brazil, Argentina, Uruguay, Venezuela and Colombia were analyzed for the bm86 and bm95 gene. A fragment of cDNA of 794 base pairs of the parasite larvae, included between nucleotides 278-1071s, was amplified and cloned on the pGEM-T vector. Two random clones were sequenced for each population and the nucleotides 278-1071 and predicted amino acid sequences compared with the bm86 and bm95 genes. Variations from 1.76 to 3.65% were detected in the nucleotides sequence when compared with the homologous sequence of the bm86 gene and a 3.4-6.08% in the homologous amino acid sequence of the Bm86 protein. When the sequences obtained were compared with the bm95 gene, variations from 0.50 to 3.15% were detected. Variations from 1.14 to 4.56% were detected for the Bm95 protein homologous sequences in the deduced amino acid sequence. Only five of the 30 strains analyzed presented two different types of alleles expressed and the two alleles of the Alegre population and allele 1 of the Betim population were the most divergent of all those analyzed.

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Section: Strainscontrasting

confidence: 74%

Polymorphism of the bm86 Gene in South American Strains of the Cattle Tick Boophilus microplus

et al. 2005

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“…Tests to differentiate strains and genotypes have been developed for Theileria (Bishop et al, 1993), Cryptosporidium (Tanrverdi et al, 2002;Giles et al, 2002), Giardia (Amar et al, 2002;Bertrand et al, 2005;Lee et al, 2006;Almeida et al, 2010), Rhipicephalus (Passos et al, 1999), Toxoplasma (Echavarria et al, 2000Alfonso et al, 2009) and Trypanosoma (Requena et al, 1992;Stothard et al, 1997Stothard et al, , 1999da Silveira Pinto et al, 2000;Ventura et al, 2002;Burgos et al, 2005;Rodrigues et al, 2010). The routine use of polymorphic markers to monitor and characterise isolates, has generally not been adopted in parasite biology.…”

Section: Assessment Of Intra-genomic Variation Determining Organism Cmentioning

confidence: 98%

Molecular diagnosis of infections and resistance in veterinary and human parasites

Hunt

2011

Veterinary Parasitology

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“…A basic awareness on genetic diversity of tick population help in understanding the susceptibility or resistance nature of ticks for effective control as well as production of vaccines (Passos et al 1999). The molecular techniques or tools that are dramatically advanced the knowledge on the control of ticks and the diagnosis of tick-born diseases (Stiller 1992).…”

Section: Introductionmentioning

confidence: 99%

Detection of genetic variability in various isolates of cattle tick, Boophilus microplus from Tamil Nadu, India using PCR-RAPD analysis

et al. 2012

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The genomic DNA from ten isolates of the cattle tick, Boophilus microplus collected in and around Chennai, India, was analyzed by random amplified polymorphic DNA (RAPD) using PCR. Selected five random primers were used for the study of genetic variability among different isolates of B. microplus. A high degree of genetic polymorphism with a different pattern of RAPD profiles for each tick isolate was detected with all these random primers. This variability was also confirmed by similarity coefficient values and dendrogram which were performed using mean RAPD profiles for all the primers between various isolates of ticks. The findings suggest the existence of a complex genotypic diversity of the tick B. microplus in an endemic region such as Chennai.

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Detection of genomic variability in different populations of the cattle tick Boophilus microplus in southern Brazil

Cited by 19 publications

References 26 publications

Polymorphism of the bm86 Gene in South American Strains of the Cattle Tick Boophilus microplus

Polymorphism of the bm86 Gene in South American Strains of the Cattle Tick Boophilus microplus

Molecular diagnosis of infections and resistance in veterinary and human parasites

Detection of genetic variability in various isolates of cattle tick, Boophilus microplus from Tamil Nadu, India using PCR-RAPD analysis

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