Detection of genetically modified organisms in soy products sold in Turkish market

Abstract

PCR-based technique for GMO detection is the most reliable choice because of its high sensitivity and specificity. As a candidate of the European union, Turkey must comply with the rules for launching into the market, traceability, and labeling of GMOs as established by Eu legislation. Therefore, the objective of this study is to assess soybean products in the Turkish market to verify compliance with legislation using qualitative Polymerase Chain Reaction (PCR) assay to detect the presence of GM soybean and to quantify its amount of GM soybean in the samples tested positive using real-time PCR. DNA extracted by the modified CTAB method was properly used for PCR amplification of food materials. The amplification of a 118 bp DNA fragment of the lectin gene from soybean by PCR was successfully achieved in all samples. The GMO screening was based on the detection of 35S promoter and NOS terminator sequences. The GM positive samples were subjected to detection of Roundup Ready TM soybean (RR) using quantitative real-time PCR. It was found that 100% of the tested food samples contained less than 0.1 per cent of EPSPS gene.

Detection of genetically modified DNA in processed maize and soybean products in Nigeria

“…The A260/280 ratio obtained for all the samples ranged between 1.64 and 1.88, which indicated that they are of acceptable quality. This result is consistent with reports of previous studies which extracted DNA from processed food products (Lipp et al, 1999;Cardarelli et al, 2005;Greiner and Konietzny, 2008;Mandaci et al, 2014). According to Jasbeer et al (2008), CTAB DNA extraction method is efficient and widely used for the extraction of pure DNA plants and plant derived food products due to its ability to provide effectively separate plant DNA from polysaccharides.…”

Monitoring the prevalence of genetically modified (GM) soybean in Turkish food and feed products

“…CaMV 35S & tNOS were also found in 5/9 soy samples collected in 2009 (Mandaci, Çakir, Turgut-Kara, Meriç, & Ari, 2014). These two reports form a useful counterpoint to our study in that their samples were mostly collected before the current Biosafety Law prohibiting unapproved GMOs came into effect (September 2010).…”

“…They were then spun down at relative centrifugal force of 16,000 g in a microcentrifuge for 10 min, and the supernatant was extracted twice with chloroform to remove protein. The aqueous (upper) phase was then incubated with 2 volumes of CTAB precipitation solution, after which the CTAB protocol was followed as previously described (Mafra et al, 2008). When necessary two or more repeat DNA extractions from each sample were carried out to increase DNA yield.…”

Threshold Level and Traceability of Roundup Ready Soybean in Tofu Production

“…Many studies have dealt with the detection of GMOs in commercially available raw and processed foods in different countries: Brazil (8), Canada (9), Malaysia (10), Serbia (11), Hungary (12), Turkey (13,14) and Portugal (15). Moreover, the most recent studies have focused on trac- ing DNA or proteins in certain food products during the manufacturing (16)(17)(18)(19).…”