Detection of Genetic Variation and Genetic Diversity in Two Indian Mudskipper Species (Boleophthalmus boddarti, B. dussumieri) using RAPD Marker

Abstract

Due to the environmental changes and habitat destruction the mudskipper fish population is decreasing in recent years. To predict the fish population structure, frequent manual survey and molecular methods are widely used. Molecular markers such as RAPD, microsatellite, allozyme, D-loop haplotype are frequently adopted to assess the population structure of an organism. In this study tenarbitrary primers were screened to estimate the genetic relationships and diversity of two mudskipper species (Boleophthalmus boddarti and B. dussumieri) in Vellar estuary, Tamilnadu, India. By this RAPD marker study, the genetic diversity (H) in B. boddarti was more (0.0116 ± 0.0066) than in B. dussumieri (0.0056 ± 0.0024) in Vellar estuary (India). The genetic distance between B. boddarti and B. dussumieri was 1.7943. By observing the species specific bands and the phylogenetic analysis it is revealed that these two species clearly deviated into separate clusters emphasizing the distinct species status.

Genetic diversity of blue-spotted mudskipper (Boleophthalmus boddarti) populations in Gulf of Thailand

“…For B. boddarti, relatively little information is available on its genetic diversity and structure. Only one study on genetic variation and diversity between B. boddarti, and B. dussumeiri from the Vellar estuary mangrove, India has been reported that random amplified polymorphic DNA genetic markers were used and showed that the value (mean ± SD) for genetic diversity in B. boddarti (H = 0.0116 ± 0.0066) was higher than that in B. dussumeiri (H = 0.0056 ± 0.0024) (Ramanadevi et al, 2013).…”