Detection of genetic diversity in closely related bread wheat using microsatellite markers

Abstract

Wheat microsatellites (WMS) were used to estimate the extent of genetic diversity among 40 wheat cultivars and lines, including mainly European elite material. The 23 WMS used were located on 15 different chromosomes, and revealed a total of 142 alleles. The number of alleles ranged from 3 to 16, with an average of 6.2 alleles per WMS. The average dinucleotide repeat number ranged from 13 to 41. The correlation coefficient between the number of alleles and the average number of repeats was only slight (r s = 0.55). Based on percentage difference a dendrogram is presented, calculated by the WMS-derived data. All but two of the wheat cultivars and lines could be distinguished. Some of the resulting groups are strongly related to the pedigrees of the appropriate cultivars. Values for co-ancestry (f) of 179 pairs of cultivars related by their pedigrees (f[Symbol: see text]0.1) averaged 0.29. Genetic similarity (GS) based on WMS of the same pairs averaged 0.44. The rank correlation for these pairs was slight, with r s = 0.55, but highly significant (P<0.001). The results suggest that a relatively small number of microsatellites can be used for the estimation of genetic diversity and cultivar identification in elite material of hexaploid bread wheat.

AB-QTL analysis in winter wheat: I. Synthetic hexaploid wheat (T. turgidum ssp. dicoccoides × T. tauschii) as a source of favourable alleles for milling and baking quality traits

“…DNA from parental and AB lines was isolated according to a modified procedure of Plaschke et al (1995). The DNA was isolated from foliage at the BBCH 29 stage (Bleiholder et al 1991) collected in bulk from single leaves from ten different plants per BC 2 F 3:4 line grown in the field.…”

Experimental Estimation of Mutation Rates in a Wheat Population With a Gene Genealogy Approach

“…Despite a reduced genetic effective size, global diversity at microsatellite markers increased in this population, suggesting that microsatellite diversity should be used with caution as an indicator in biodiversity conservation issues. B ECAUSE microsatellite markers (tandemly repeated DNA motifs of 1-6 bp in length) are highly polymorphic and since they are distributed across the whole genome (Wu and Tanksley 1993;Plaschke et al 1995;Pejic et al 1998), they constitute a powerful tool to assess the level of genetic differentiation within and among experimental or natural populations at different generations. The high degree of polymorphism at microsatellite markers is directly related to their underlying mutation rates, which can be explained by two mutational mechanisms: polymerase slippage during DNA replication and unequal crossing over during recombinationbut not excluding SNP mutations at a lower rate.…”

QTL Analysis in Aromatic Rice of Assam, India

“…Skewness (K 3 ), the third degree statistics and Kurtosis (K 4 ), the fourth degree statistics were estimated according to Snedecor and Cochran [9] to understand the nature of distribution of different traits as described by Fisher et al [10] and number of genes controlling the traits as by Robson respectively. [11] Molecular marker analysis DNA from each F 2 plant was extracted from fresh leaves following the protocol of Plasckhe et al [12] with slight modification. A total of 137 primer pairs were selected and primer sequences were obtained from Gramene (http://www.gramene.org).…”