Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

Gowayed, Salah M.; El-Moneim, Diaa Abd

doi:10.1371/journal.pone.0248890

Cited by 19 publications

(12 citation statements)

References 52 publications

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“…It is clear that the total carbohydrate content was higher at 50 mM NaCl (331.309 mg/gDW). Whereas total carbohydrate content dropped at 150 and 200 mM NaCl by 262.65 and 224.68 mg/gDW, respectively, which is agreed with Gowayed and Abd El-Moneim (2021) results, who stated that total carbohydrate content has not changed at 150 mM NaCl. Moreover, Ali et al (2021) recorded a reduction in the total carbohydrate in the saline condition, which agrees with our results.…”

Section: Discussionsupporting

confidence: 91%

The effect of salinity stress on the Phaseolus vulgaris L. plant

Al-huraby¹,

Bafeel²

2022

African Journal of Biological Sciences

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Soil salinity is one of the world's most important problems. Many studies have tried to find a solution to this problem. Therefore, this report aimed to determine the tolerance of salt concentrations for pole bean (Phaseolus vulgaris L.) plant growth naturally. The current experiment examined the effect of irrigation with saline water on seed germination and seedling growth. Seeds were grown in media containing 0.0, 50, 100, 150, and 200 mM NaCl. Therefore, the number of germinated seeds decreased according to the media concentration. 15-day-old pole bean seedlings were irrigated with saline water at different concentrations (0.0, 50, 100, 150, and 200 mM NaCl). Later, seedlings are watered with tap water for 15 days. After that, the soil and plant shoot samples were collected for analysis purposes. Our results showed that soil pH, EC, and moisture enhanced as salinity levels increased. The responses of pole bean plants to salt stress were documented. Pole bean shoots have gradually increased both soluble carbohydrates and protein content, with the increase of salt levels and free amino acids compared to control. However, photosynthetic pigments and carotenoids of the pole bean leaves were higher in control than under salt concentrations.

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Section: Discussionsupporting

confidence: 91%

The effect of salinity stress on the Phaseolus vulgaris L. plant

Al-huraby¹,

Bafeel²

2022

African Journal of Biological Sciences

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“…Remarkably, Sohag-5 was characterized as salt susceptible. The same genotype was previously reported as a salt susceptible genotype at the germination stage by Gowayed and Abd El-Moneim ( 2021 ).…”

Section: Discussionmentioning

confidence: 55%

High-LD SNP markers exhibiting pleiotropic effects on salt tolerance at germination and seedlings stages in spring wheat

et al. 2022

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Key message Salt tolerance at germination and seedling growth stages was investigated. GWAS revealed nine genomic regions with pleiotropic effects on salt tolerance. Salt tolerant genotypes were identified for future breeding program. Abstract With 20% of the irrigated land worldwide affected by it, salinity is a serious threat to plant development and crop production. While wheat is the most stable food source worldwide, it has been classified as moderately tolerant to salinity. In several crop plants; such as barley, maize and rice, it has been shown that salinity tolerance at seed germination and seedling establishment is under polygenic control. As yield was the ultimate goal of breeders and geneticists, less attention has been paid to understanding the genetic architecture of salt tolerance at early stages. Thus, the genetic control of salt tolerance at these stages is poorly understood relative to the late stages. In the current study, 176 genotypes of spring wheat were tested for salinity tolerance at seed germination and seedling establishment. Genome-Wide Association Study (GWAS) has been used to identify the genomic regions/genes conferring salt tolerance at seed germination and seedling establishment. Salinity stress negatively impacted all germination and seedling development parameters. A set of 137 SNPs showed significant association with the traits of interest. Across the whole genome, 33 regions showed high linkage disequilibrium (LD). These high LD regions harbored 15 SNPs with pleiotropic effect (i.e. SNPs that control more than one trait). Nine genes belonging to different functional groups were found to be associated with the pleiotropic SNPs. Noteworthy, chromosome 2B harbored the gene TraesCS2B02G135900 that acts as a potassium transporter. Remarkably, one SNP marker, reported in an early study, associated with salt tolerance was validated in this study. Our findings represent potential targets of genetic manipulation to understand and improve salinity tolerance in wheat.

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“…Abou-Deif, et al, [54] used the ISSR molecular marker to estimate the genetic diversity between some Egyptian wheat genotypes. But El-Moneim, [55] and Gowayed and El-Moneim [9] used ISSR and SCoT primers to determine and evaluate some Egyptian wheat genotypes tolerant to drought and salt stress, respectively. But our current study used different primers for SCoT in genetic diversity and evaluation of Egyptian wheat genotypes' resistance to stem and leaf rust diseases.…”

Section: Plos Onementioning

confidence: 99%

“…Molecular marker techniques are considered a useful tool for the determination, characterization, and assessment of genetic diversity among different organisms at the species-level (Hanaa, et al 2020). SCoT and SRAP markers are effectively used in various species of microorganisms to test the genetic diversity, structure, identification of cultivars, quantitative trait loci (QTL) mapping and DNA fingerprinting [8,9]. However, the genetic diversity assessment between and within the species of Carthamus was carried out by SRAP [10,11].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity, antifungal evaluation and molecular docking studies of Cu-chitosan nanoparticles as prospective stem rust inhibitor candidates among some Egyptian wheat genotypes

et al. 2021

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Wheat has a remarkable importance among cereals worldwide. Wheat stem and leaf rust constitute the main threats that destructively influence grain quality and yield production. Pursuing resistant cultivars and developing new genotypes including resistance genes is believed to be the most effective tool to overcome these challenges. This study is the first to use molecular markers to evaluate the genetic diversity of eighteen Egyptian wheat genotypes. Moreover, the molecular docking analysis was also used to assess the Cu-chitosan nanoparticle (CuChNp) treatment and its mode of action in disease control management. The tested genotypes were categorized into two main cluster groups depending on the similarity matrix, i.e the most resistant and susceptible genotypes to stem and leaf rust races. The results of SCoT primers revealed 140 polymorphic and 5 monomorphic bands with 97% polymorphism. While 121 polymorphic and 74 monomorphic bands were scored for SRAP primers (99% polymorphism). The genotypes Sakha 94, Sakha 95, Beni Sweif 4, Beni Sweif 7, Sohag 4 and Sohag 5 were resistant, while Giza 160 was highly susceptible to all stem rust races at the seedling stage. However, in the adult stage, the 18 genotypes were evaluated for stem and leaf rust-resistant in two different locations, i.e. Giza and Sids. In this investigation, for the first time, the activity of CuChNp was studied and shown to have the potential to inhibit stem and leaf rust in studied Egyptian wheat genotypes. The Spraying Cu-chitosan nanoparticles showed that the incubation and latent periods were increased in treated plants of the tested genotypes. Molecular modeling revealed their activity against the stem and leaf rust development. The SRAP and SCoT markers were highly useful tools for the classification of the tested wheat genotypes, although they displayed high similarities at the morphological stage. However, Cu-chitosan nanoparticles have a critical and effective role in stem and leaf rust disease control.

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Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

Cited by 19 publications

References 52 publications

The effect of salinity stress on the Phaseolus vulgaris L. plant

The effect of salinity stress on the Phaseolus vulgaris L. plant

High-LD SNP markers exhibiting pleiotropic effects on salt tolerance at germination and seedlings stages in spring wheat

Genetic diversity, antifungal evaluation and molecular docking studies of Cu-chitosan nanoparticles as prospective stem rust inhibitor candidates among some Egyptian wheat genotypes

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