Detection of genetic determinants that define the difference in photoperiod sensitivity of Triticum aestivum L. near-isogenic lines

Kiseleva, A. A.; Eggi, E. E.; Koshkin, V. A.; Sitnikov, M. N.; Röder, Marion S.; Салина, Е. А.; Potokina, Elena

doi:10.1134/s102279541405007x

Cited by 2 publications

(8 citation statements)

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“…Lines “Ppd-m” and “Ppd-w” differ from their sister lines by the introgressions on the 2B chromosome from the Sonora variety. This difference was shown previously using SSR analysis [ 14 ]. An analysis of specific molecular markers also demonstrated that the near-isogenic lines (NILs) have recessive alleles of Ppd-D1 and Ppd-A1 [ 14 ].…”

Section: Methodssupporting

confidence: 55%

“…This difference was shown previously using SSR analysis [ 14 ]. An analysis of specific molecular markers also demonstrated that the near-isogenic lines (NILs) have recessive alleles of Ppd-D1 and Ppd-A1 [ 14 ]. DNA was extracted from the seedlings using the GeneJET Plant Genomic DNA Purification Mini Kit (Thermo Fisher Scientific, Lithuania) according to the manufacturer’s protocol.…”

Section: Methodssupporting

confidence: 55%

“…We have hypothesized that the Ppd-B1 gene in the PI Sonora, “Ppd-m” and “Ppd-w” is characterized by not only an increased copy number but also by a nucleotide polymorphism in one of the copies because NILs “Ppd-m” and “Ppd-w” flowered slightly later than the line with the same background and introgressed Ppd-D1a gene [ 14 ]. Therefore, we analyzed the sequences of the Ppd-B1 distinct copies.…”

Section: Resultsmentioning

confidence: 99%

“…SSR genotyping of the full genome of NILs was performed in previous study [ 14 ]. Unfortunately, there was a mistake in illustration described “Ppd-w” and “Ppd-0 w ” lines.…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we have hypothesized that the Ppd-B1 allele in the investigated lines might be characterized by sequence mutations in addition to copy number variations [ 14 ]. To investigate all possible sequence variations, we developed primers with a coding region that is specific to all Ppd-1 genes.…”

Section: Methodsmentioning

confidence: 99%

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Features of Ppd-B1 expression regulation and their impact on the flowering time of wheat near-isogenic lines

2017

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BackgroundPhotoperiod insensitive Ppd-1a alleles determine early flowering of wheat. Increased expression of homoeologous Ppd-D1a and Ppd-A1a result from deletions in the promoter region, and elevated expression of Ppd-B1a is determined by an increased copy number.ResultsIn this study, using bread wheat cultivars Sonora and PSL2, which contrast in flowering time, and near-isogenic lines resulting from their cross, “Ppd-m” and “Ppd-w” with Ppd-B1a introgressed from Sonora, we investigated the putative factors that influence Ppd-B1a expression. By analyzing the Ppd-B1a three distinct copies, we identified an indel and the two SNPs, which distinguished the investigated allele from other alleles with a copy number variation.We studied the expression of the Ppd-A1, Ppd-B1a, and Ppd-D1 genes along with genes that are involved in light perception (PhyA, PhyB, PhyC) and the flowering initiation (Vrn-1, TaFT1) and discussed their interactions. Expression of Ppd-B1a in the “Ppd-m” line, which flowered four days earlier than “Ppd-w”, was significantly higher. We found PhyC to be up-regulated in lines with Ppd-B1a alleles. Expression of PhyC was higher in “Ppd-m”. Microsatellite genotyping demonstrated that in the line “Ppd-m”, there is an introgression in the pericentromeric region of chromosome 5B from the early flowering parental Sonora, while the “Ppd-w” does not have this introgression. FHY3/FAR1 is known to be located in this region. Expression of the transcription factor FHY3/FAR1 was higher in the “Ppd-m” line than in “Ppd-w”, suggesting that FHY3/FAR1 is important for the wheat flowering time and may cause earlier flowering of “Ppd-m” as compared to “Ppd-w”.ConclusionsWe propose that there is a positive bidirectional regulation of Ppd-B1a and PhyC with an FHY3/FAR1 contribution.The bidirectional regulation can be proposed for Ppd-A1a and Ppd-D1a. Using in silico analysis, we demonstrated that the specificity of the Ppd-B1 regulation compared to that of homoeologous genes involves not only a copy number variation but also distinct regulatory elements.Electronic supplementary materialThe online version of this article (10.1186/s12870-017-1126-z) contains supplementary material, which is available to authorized users.

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Section: Methodssupporting

confidence: 55%

Section: Methodssupporting

confidence: 55%

Section: Resultsmentioning

confidence: 99%

“…SSR genotyping of the full genome of NILs was performed in previous study [ 14 ]. Unfortunately, there was a mistake in illustration described “Ppd-w” and “Ppd-0 w ” lines.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations