Detection of genes of RNA viruses from freshly biopsied gastric mucosa by reverse transcription polymerase chain reaction

Abstract

The reverse transcription polymerase chain reaction (RT-PCR) was performed to detect genes of RNA viruses in the freshly biopsied gastric mucosa of seven patients with low gastric acidity. Although nucleoprotein genes of Sendai virus and hemmaglutinin genes of influenza virus A were not detected, nucleoprotein genes of influenza virus B were detected in samples from three of the seven patients. The first patient had had antrectomy and vagotomy for gastric ulcer, the second patient was receiving a histamine type 2 receptor blocker for gastritis, and the third patient was receiving a proton pump inhibitor for gastric ulcer. Virus isolation from gastric mucosa and from gargles was negative for all seven patients. These findings suggest that genes of influenza viruses may exist in the gastric mucosa of patients with low gastric acidity.

Detection of type B influenza virus genes from biopsied gastric mucosa

“…These results indicate the presence of the influenza virus gene in the gastric mucosa and suggest the persistent infection of the gastric mucosa with type B influenza viruses. There seem to be two possible mechanisms of influenza persistence in gastric mucosa; (1) in the light of our finding that influenza virus was not isolated from gastric mucosa, 6 it is possible that an undetectably small amount of infectious virions may be produced and reinfect the gastric mucosa, (2) influenza viral genes may replicate in propagating epithelial cells without the formation of infectious virions. In the latter case, influenza viral A820T and A829T were missense mutations (Ile-to-Phe and Ile-to-Leu, respectively).…”

“…These patients included the 3 who had been found to be positive for the influenza viral NP gene in our previous study, 37 months earlier. 6 None of Background. We previously reported the existence of type B influenza genes in the gastric mucosa of three patients.…”

“…We have previously reported the existence of type B influenza nucleoprotein (NP) genes in the gastric mucosa of three patients. 6 In the present study, to further characterize gastric mucosal infection with type B influenza virus, in which the natural host is limited to humans, we performed reverse transcription-polymerase chain reaction (RT-PCR) to detect type B influenza virus genes from the gastric mucosa of 80 patients. Endoscopic examinations were performed in the summer to avoid contamination from the respiratory tract.…”

Superoxide Anion Production by Granulocytes Incubated with HeLa 229 Cells Persistently Infected with Influenza Virus B/Lee/40

“…Thus, in acute infection, granulocytes with an enhanced inflammatory potential cause airway injury, obstruction, and responsiveness during a viral respiratory infection by the production of free radicals [5]. Another form of influenza virus infection, persistent influenza infection, can take place in cultured cells [6,7] and in vivo [8,9], although reactivation of persistent influenza viral genes has not been detected in humans. The pathogenic roles of granulocytes in influenza virus persistent infection are yet to be investigated.…”

Characterization of HeLa Cells Persistently Infected with Influenza Virus B/Lee/40 with Respect to Telomerase Activity and Apoptosis

“…The latter two are have a positive-sense RNA genome [1,2]. Infections with type A and type B influenza viruses are usually acute and lytic, while persistent nonlytic infection can occur in cultured cells [3][4][5][6][7][8] and probably in vivo [9][10][11][12]. In the cells acutely infected with influenza viruses, programmed cell death (apoptosis) is induced [13].…”