Detection of gene copy number fluctuations with microarrays using genomic representations

Transforming science: cancer gene identification

“…For this reason, many technologies have been developed to identify regions of the genome that have undergone copy number changes in tumors, and which specific genes in these regions function as cancer genes. There has been a steady increase in genome coverage and resolution of these technologies, which include comparative genome hybridization (CGH) [43], array CGH [44,45], representative differential analysis (RDA) [46], representative oligonucleotide microarray analysis (ROMA) [47] and digital karyotyping [48,49] (see Glossary for description of these techniques). These approaches have revealed new regions of recurrent chromosomal amplifications and deletions in tumors [50,51].…”